Abstract
It has been demonstrated that the mycelium of Curvularia lunata at the end of the logarithmic growth phase displays a maximal 11β-hydroxylase activity towards cortexolone (4–6 g/l) used for transformation as a microcrystalline suspension in phosphate buffer. The mycelium at a later stage of fungal growth displays an elevated 14α-hydroxylase activity, necessary for generation of 14α-hydroxyandrostenedione. The effects of different forms of substrate added to the reaction mixture, age and concentration of mycelium, and fungal clones tolerant to salts of heavy metals (0.35–0.5%) were studied to remove the side 14α-hydroxylation, accompanying the main cortexolone transformation. Mycelia of the fungal clones tolerant to Co2+ and Cu2+ displayed a weak hydroxylase activity or its complete absence and an elevated content of melanin, the biosynthesis of which is intensified under adverse conditions. The results obtained suggest that the transformation of steroids by the studied C. lunata strain is a detoxication of foreign compounds.
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Abbreviations
- AD :
-
androstenedione (androst-4-en-3,17-dione)
- 14α-OH-AD:
-
14α-hydroxy-AD
- substance F:
-
hydrocortisone or Kendall’s substance F (pregn-4-en-1117,21-trihydroxy-3,20-dione)
- substance S:
-
cortexolone or Reichstein’s substance S (pregn-4-en-17,21-dihydroxy-3,20-dione)
- 14α-OH-substance S:
-
14α-hydroxy-substance S
References
Charney, W. and Herzog, H.L., Microbial Transformations of Steroids, New York: Academic, 1967.
Akhrem, A.A. and Titov, Yu.A., in Steroidy i mikroorganizmy (Steroids and Microorganisms), Moscow: Nauka, 1972.
Angelova, B.A., Sukhodol’skaya, G.V., and Koshcheenko, K.A., Mikrobiologiya, 1985, vol. 54, no. 5, pp. 704–709.
Sukhodol’skaya, G.V., Angelova, B.A., and Koshcheenko, K.A., Prikl. Biokhim. Mikrobiol., 1986, vol. 22, no. 2, pp. 226–237.
Turuta, A.M., Kamernitskii, A.V., Bogdanov, V.S., Komarova, G.V., and Koshcheenko, K.A., Khim.-Farm. Zh., 1990, vol. 24, no. 6, pp. 52–55.
Umnova, E.F., Ryzhkova, V.M., and Vorob’eva, L.I., Khim.-Farm. Zh., 1990, vol. 24, no. 6, pp. 56–58.
Sukhodol’skaya, G.V., Angelova, B.A., Koshcheenko, K.A., and Skryabin, G.K., Prikl. Biokhim. Mikrobiol., 1991, vol. 27, no. 5, pp. 701–710.
Umnova, E.F. and Vorob’eva, L.I., Biol. Nauki, 1992, no. 3, pp. 122–126.
Sukhodol’skaya, G.V., Gulevskaya, S.A., Chincholkar, S.B., Koshcheenko, K.A., Dzhoshi, A.K., Bikhari, V., and Basu, S.K., Prikl. Biokhim. Mikrobiol., 1996, vol. 32, no. 1, pp. 69–77.
Spassov, G., Pramatarova, V., and Wandrey, C., Appl. Microbiol. Biotechnol., 1996, vol. 46, pp. 481–488.
Shuvalova, S.D., Gabinskaya, K.N., Popova, E.V., Savinova, T.S., and Andryushina, V.A., Khim.-Farm. Zh., 2001, vol. 35, no. 5, pp. 44–46.
US Patent no. 6828120, 2004.
Voishvillo, N.E., Andryushina, V.A., Savinova, T.S., and Stytsenko, T.S., Appl. Biochem. Microbiol, 2004, vol. 40, pp. 463–469.
Ambrus, G., Jekkel, A., Ilkoy, E., Horvath, G., and Bocksei, Z., Steroids, 1995, vol. 60, no. 4, pp. 626–629.
Gadd, G.M., New Phytol., 1993, vol. 124, pp. 25–60.
Samantha, T.R. and Ghosh, D.K., J. Steroid Biochem., 1983, vol. 28, pp. 327–332.
Breskvar, K. and Hudnik-Plevnik, T., J. Steroid Biochem, 1978, vol. 9, no. 2, pp. 131–134.
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Original Russian Text © V.V. Yaderets, V.A. Andryushina, Yu.E. Bartoshevich, A.G. Domracheva, M.I. Novak, T.S. Stytsenko, N.E. Voishvillo, 2007, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 6, pp. 697–702.
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Yaderets, V.V., Andryushina, V.A., Bartoshevich, Y.E. et al. A study of steroid hydroxylation activity of Curvularia lunata mycelium. Appl Biochem Microbiol 43, 620–624 (2007). https://doi.org/10.1134/S0003683807060099
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DOI: https://doi.org/10.1134/S0003683807060099