Abstract
Gene egl2 of secreted endo-(1–4)-β-glucanase of glycosyl hydrolase family 5 of the mycelial fungus Penicillium canescens was cloned. The gene was expressed in P. canescens under control of a strong promoter of the bgaS gene encoding β-galactosidase of P. canescens, and endoglucanase producing strains were obtained. Chromatographically purified recombinant 48 kDa protein had pH and temperature optima 3.4 and 60°C, respectively, exhibited specific activity of 33 IU, and had K m and V max in CM-cellulose hydrolysis of 10.28 g/liter and 0.26 μmol/sec per mg, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- CMC:
-
CM-cellulose
- DSC:
-
differential scanning calorimetry
References
Sinitsyn, A. P., Gusakov, A. V., and Chernoglazov, V. M. (1995) Bioconversion of Lignocellulose Materials [in Russian], MGU Publishing House, Moscow.
Clarke, A. J. (1997) Biodegradation of Cellulose. Enzymology and Biotechnology, Technomic Publisher Co., Lancaster.
Aleksenko, A. Y., Makarova, N. A., Nikolaev, I. V., and Clutterbuck, A. J. (1995) Curr. Genet., 28, 474–478.
Nikolaev, I. V., Bekker, O. B., Serebryanyi, V. A., Chulkin, A. M., and Vinetskii, Yu. P. (1999) Biotekhnologiya, 3, 3–13.
Nikolaev, I. V., and Vinetskii, Yu. P. (1998) Biochemistry (Moscow), 63, 1294–1298.
Laemmli, U. K. (1970) Nature, 227, 680–685.
Somogyi, M. (1952) J. Biol. Chem., 195, 19–28.
Nelson, N. (1944) J. Biol. Chem., 153, 375–380.
Varfolomeev, S. D., and Gurevich, K. G. (1999) Biokinetics: Practical Course [in Russian], FIAR-PRESS Publishing House, Moscow.
Siebert, P. D., Chenchik, A., Kellogg, D. E., Luk’yanov, K. A., and Luk’yanov, S. A. (1995) Nucleic Acids Res., 23, 1087–1088.
Rambosek, J., and Leach, J. (1987) Crit. Rev. Biotechnol., 6, 357–393.
Unkles, S. E., Campbell, E. I., Punt, P. J., Hawker, K. L., Contreras, R., Hawkins, A. R., van den Hondel, C. A., and Kinghorn, J. R. (1992) Gene, 111, 149–155.
Schulein, M. (1997) J. Biotechnol., 57, 71–81.
Takashima, S., Takashima, S., Iikura, H., Nakamura, A., Hidaka, M., Masaki, H., and Uozumi, T. (1998) J. Biotechnol., 65, 163–171.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A. M. Chulkin, D. S. Loginov, E. A. Vavilova, A. R. Abyanova, I. N. Zorov, S. A. Kurzeev, O. V. Koroleva, S. V. Benevolenskii, 2009, published in Biokhimiya, 2009, Vol. 74, No. 6, pp. 805–813.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-372, May 10, 2009.
Rights and permissions
About this article
Cite this article
Chulkin, A.M., Loginov, D.S., Vavilova, E.A. et al. Enzymological properties of endo-(1–4)-β-glucanase Eg12p of Penicillium canescens and characteristics of structural gene egl2 . Biochemistry Moscow 74, 655–662 (2009). https://doi.org/10.1134/S0006297909060108
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297909060108