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Formation of bioactive highly porous polymer matrixes for tissue engineering

  • Materials for Insuring Human Life Activity and Environment Protection
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Inorganic Materials: Applied Research Aims and scope

Abstract

The processes of fabrication of highly porous (60–90 vol %) bioactive heparin-containing polylactic scaffolds in supercritical carbon dioxide followed by their hydrophilization by dielectric barrier discharge plasma treatment in the atmosphere were studied. A homogeneous distribution of heparin (HP) over the polymer volume was demonstrated by spatially resolved Raman scattering (RS) spectroscopy. The kinetics of heparin release from the scaffolds in distilled water was studied by spectrophotometry. A virtually linear increase in heparin concentration in a solution was shown from the second until the 15th day of experiments. Comparative in vitro study of cytotoxicity and matrix properties of pure polymer and heparin-containing scaffolds using NIH 3T3 mice fibroblast cultures demonstrated a positive effect of heparin distribution over the polylactic scaffolds on both cell adhesion and proliferation.

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Authors and Affiliations

  1. Institute of Laser and Information Technologies, Russian Academy of Sciences, ul. Svyatoozerskaya 1, Shatura, Moscow oblast, 140700, Russia

    S. E. Bogorodskii, L. I. Krotova, S. A. Minaeva, A. V. Mironov & V. K. Popov

  2. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, ul. Shchukinskaya 1, Moscow, 123182, Russia

    V. N. Vasilets, E. A. Nemets, V. A. Surguchenko & V. I. Sevast’yanov

  3. Institute of Biomedical Research and Technology, per. Bolshoi Tishinskii 43/20b, Moscow, 123557, Russia

    E. A. Nemets & V. A. Surguchenko

Authors
  1. S. E. Bogorodskii
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  2. V. N. Vasilets
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  3. L. I. Krotova
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  4. S. A. Minaeva
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  5. A. V. Mironov
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  6. E. A. Nemets
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  7. V. A. Surguchenko
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  8. V. K. Popov
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  9. V. I. Sevast’yanov
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Correspondence to S. E. Bogorodskii.

Additional information

Original Russian Text © S.E. Bogorodskii, V.N. Vasilets, L.I. Krotova, S.A. Minaeva, A.V. Mironov, E.A. Nemets, V.A. Surguchenko, V.K. Popov, V.I. Sevast’yanov, 2013, published in Perspektivnye Materialy, 2013, No. 5, pp. 44–54.

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Bogorodskii, S.E., Vasilets, V.N., Krotova, L.I. et al. Formation of bioactive highly porous polymer matrixes for tissue engineering. Inorg. Mater. Appl. Res. 4, 448–456 (2013). https://doi.org/10.1134/S2075113313050043

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  • DOI: https://doi.org/10.1134/S2075113313050043

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