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Age-dependent changes of mitochondrial functions in Ca2+-induced opening of permeability transition pore

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Mitochondria are intracellular organelles, which provide cells with energy and participate in multiple processes of cell vital functions. Within one of the numerous theories of aging, dysfunction of mitochondria is considered to lead to tissue degeneration and induce the initial stage in developing of degenerative diseases. Since mitochondria play a clue role in apoptosis/necrosis processes, it was suggested that dysfunction of mitochondria observed under aging is related with disturbance of programmed cell death regulation. In the present study, a comparative examination of parameters of the functional states of mitochondria isolated from young (2–3-months old) and old (20–22-months old) rats under conditions of opening of unselective pore (PTP, permeability transition pore) has been performed. Ca2+ accumulation rate in mitochondria isolated from old rats was found to be decreased by 25–30%, threshold calcium concentration was lowered to 50%, and the swelling of mitochondria loaded by calcium was stimulated 3–4-fold. Production of reactive oxygen species (ROS) has been also determined in these mitochondria. In old mitochondria superoxide anion level was increased. In addition, H2O2 content was found to be 2 times higher in mitochondria with PTP opened. Using electron microscopy method, a decreased amount of cristae in mitochondria was revealed under aging.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia

    O. V. Krestinina, A. G. Kruglov, D. E. Grachev, Yu. L. Baburina, Yu. V. Evtodienko, D. A. Moshkov, I. M. Santalova & T. S. Azarashvili

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  1. O. V. Krestinina
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  2. A. G. Kruglov
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  3. D. E. Grachev
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  4. Yu. L. Baburina
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  5. Yu. V. Evtodienko
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  6. D. A. Moshkov
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  7. I. M. Santalova
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  8. T. S. Azarashvili
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Correspondence to T. S. Azarashvili.

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Original Russian Text © O.V. Krestinina, A.G. Kruglov, D.E. Grachev, Yu.L. Baburina, Yu.V. Evtodienko, D.A. Moshkov, I.M. Santalova, T.S. Azarashvili, 2010, published in Biologicheskie Membrany, 2010, Vol. 27, No. 2, pp. 177–183.

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Krestinina, O.V., Kruglov, A.G., Grachev, D.E. et al. Age-dependent changes of mitochondrial functions in Ca2+-induced opening of permeability transition pore. Biochem. Moscow Suppl. Ser. A 4, 180–186 (2010). https://doi.org/10.1134/S199074781002008X

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  • DOI: https://doi.org/10.1134/S199074781002008X

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