Abstract
The goal of this work was to study cellular mechanisms of tumor progression and metastasizing. As a result of explantation of cells of rat Zajdela ascitic hepatoma, we obtained two transplantable cell cultures—monolayer (ZH-ad) and suspension (ZH-fl)—that differ in levels of cell differentiation and tumorigenicity. By using tumor-specific immune serum, we revealed tumor-associated antigens, synthesis of which is reduced or inhibited in ZH-ad cells, in outer membranes of the ZH-fl cells. Intraperitoneal injection into rat of 0.5–12 × 106 ZH-fl cells leads to development of an ascitic tumor and death of 100% of animals, whereas, in the case of administration of ZH-ad cells, to achieve a tumorigenic effect, the minimal dose needs to be elevated to 20 × 106 cells. Clonogenic analysis of the ZH-fl cells revealed three types of the formed clones—nonadhesive sphere colonies and two types of monolayer clones differing in proliferative potential, shape of colonies, and cell composition. Upon reaching a critical size, the spheres disintegrated, with separation of single cells and islands of different sizes, some of them being attached with monolayer formation. Three clonal cell lines were obtained: 1C as a result of expansion of a spherical clone and 4G and 10E from monolayer clones. We established that there is tumorigenicity of the 1C cell line, which, at a dose of 107 cells, led to the development of ascites and to the death of 50% of animals. The presented results indicate the existence in the ZH-fl cell population of tumor-initiating cells generating spherical clones—floating multicellular islets that, in culturing in the complete growth medium, are partly differentiated and are attached with monolayer formation.
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Abbreviations
- HC:
-
hepatocellular carcinoma
- 4-DAB:
-
4-demethylaminoazobensene
- TSC:
-
tumor stem cells
- CFS:
-
calf fetal blood serum
- EpCAM:
-
epithelial molecule of cell adhesion
- ZH-ad:
-
monolayer culture of Zajdela hepatoma cells
- ZH-fl:
-
suspension culture of Zajdela hepatoma cells
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Original Russian Text © N.P. Teryukova, G.I. Blinova, V.A. Ivanov, 2013, published in Tsitologiya, Vol. 55, No. 1, 2013, pp. 36–44.
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Teryukova, N.P., Blinova, G.I. & Ivanov, V.A. Zajdela hepatoma cells cultured in vitro. Cell Tiss. Biol. 7, 245–252 (2013). https://doi.org/10.1134/S1990519X13030127
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DOI: https://doi.org/10.1134/S1990519X13030127