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Clonal growth and differentiation of mesenchymal stromal cells from rat liver at different stages of embryogenesis

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Abstract

Fetal liver, during its hematopoietic activity, contains mesenchymal stromal cells (MSCs) generating its hematopoietic microenvironment. These cells are clonogenic and capable of multilineage differentiation; however, little is known about how their properties alter during embryogenesis. We compared the cloning efficiency of MSCs from rat fetal liver at 14, 16, and 20 days of development, as well as their capacity for osteo- and adipogenesis in vitro and chondrogenesis in vivo by ectopic transplantation of intact liver. The relative content of clonogenic MSCs in liver cell suspension was highest in 16-day fetuses and lowest in 20-day fetuses. Cells from 14-day fetuses exhibited high osteogenic and less apparent adipogenic and chondrogenic potential; cells from 20-day fetuses displayed weak adipogenic capacity and no osteo- or chondrogenic ability. These results show the correlation of MSC content and the cell differentiation potential with hematopoietic dynamics in developing rat liver. It may be thought that the changes we observed are related to the loss of hematopoietic activity and liver getting of definitive functions.

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Abbreviations

CFU-F:

colony forming units of fibroblasts

MSC:

mesenchymal stromal cells

AP:

alkaline phophatase

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Authors and Affiliations

  1. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    O. V. Payushina, N. N. Butorina, T. M. Nikonova, M. N. Kozhevnikova, O. N. Sheveleva & V. I. Starostin

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  1. O. V. Payushina
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  2. N. N. Butorina
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  3. T. M. Nikonova
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  4. M. N. Kozhevnikova
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  5. O. N. Sheveleva
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  6. V. I. Starostin
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Correspondence to O. V. Payushina.

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Original Russian Text © O.V. Payushina, N.N. Butorina, T.M. Nikonova, M.N. Kozhevnikova, O.N. Sheveleva, V.I. Starostin, 2011, published in Tsitologiya, 2011, Vol. 53, No. 11, pp. 859–867.

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Payushina, O.V., Butorina, N.N., Nikonova, T.M. et al. Clonal growth and differentiation of mesenchymal stromal cells from rat liver at different stages of embryogenesis. Cell Tiss. Biol. 6, 12–19 (2012). https://doi.org/10.1134/S1990519X12010075

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  • DOI: https://doi.org/10.1134/S1990519X12010075

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