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Streptomyces avermitilis: Component Composition and Properties

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Abstract

The component composition of Streptomyces avermitilis biomass was studied, including avermectin, lipids, proteins, and a polysaccharide complex (murein). It was established that the composition of lipids of S. avermitilis contains predominantly saturated fatty acids (86%), and the content of monounsaturated (palmitic and vaccine) and polyunsaturated (linoleic and α-linolenic) fatty acids is the same (approximately 7%). The presence of a significant amount of branched-chain fatty acids (isoforms)—more than 70% of the total amount of fatty acids—should be noted. A technique for isolating the polysaccharide complex from S. avermitilis biomass was developed. Using IR spectroscopy, it was found that the S. avermitilis polysaccharide complex, as well as the chitin–glucan complex isolated from the thallus of the epigeneic lichen Cladonia rangiferina, is built from chitin units (β-N-acetylglucosamine). It was shown that the polysaccharide complex (murein) has a polyampholytic nature and exhibits high sorption properties with respect to the basic (methylene blue) and acid (Congo red) dyes. It was also shown that the sorption capacity of the polysaccharide complex from S. avermitilis with respect to the studied dyes is 2.8–7.6 times higher than that of the chitin–glucan complex from Cladonia rangiferina. This indicates a significantly higher content of active functional groups (sorption centers) in the murein structure, which allows us to recommend it for use as an effective sorbent (enterosorbent).

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ACKNOWLEDGMENTS

This study was carried out using the equipment of the “Arktika” Core Facility of the Lomonosov Northern (Arctic) Federal University and the Arktika Core Facility of the Laverov Federal Center for Integrated Arctic Research, Ural Branch of the Russian Academy of Sciences.

Funding

The study was performed under the state assignment of the Laverov Federal Research Center for Integrated Arctic Research, Ural Branch of the Russian Academy of Sciences “Physicochemical, Genetic, and Morphological Bases of Adaptation of Plant Objects in the Changing Climate of High Latitudes” (no. АААА-А18-118012390231-9).

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Authors and Affiliations

  1. Laverov Federal Center for Integrated Arctic Research, Ural Branch of the Russian Academy of Sciences, 163000, Arkhangelsk, Russia

    O. S. Brovko, D. V. Zhil’tsov, A. D. Ivakhnov & M. V. Bogdanov

  2. Lomonosov Northern (Arctic) Federal University, 163002, Arkhangelsk, Russia

    A. D. Ivakhnov

Authors
  1. O. S. Brovko
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  2. D. V. Zhil’tsov
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  3. A. D. Ivakhnov
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  4. M. V. Bogdanov
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Correspondence to D. V. Zhil’tsov.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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The authors declare that they have no conflicts of interest.

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Translated by M. Batrukova

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Brovko, O.S., Zhil’tsov, D.V., Ivakhnov, A.D. et al. Streptomyces avermitilis: Component Composition and Properties. Russ J Bioorg Chem 47, 1424–1431 (2021). https://doi.org/10.1134/S1068162021070049

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