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Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study

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Abstract

Crystals of high-purity recombinant NAD+-dependent formate dehydrogenase from the higher plant Arabidopsis thaliana (AraFDH) were grown in microgravity in the Modul’-1 protein crystallization apparatus on the International Space Station. The space-grown crystals have larger sizes than those grown on Earth. X-ray diffraction data suitable for determining the three-dimensional structure were collected from the space-grown crystals to a resolution of 1.22 Å using an X-ray synchrotron source. The crystals belong to sp. gr. P43212; the unit-cell parameters are a = b = 107.865 Å, c = 71.180 Å, α = β = γ = 90°.

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References

  1. I. P. Kuranova, Poverkhnost’: Rentgen., Sinkhrotron. Neítr. Issled., No. 6, 6 (2004).

  2. E. A. Smirnova, Yu. A. Kislitsyn, N. I. Sosfenov, et al., Kristallografiya 54, 948 (2009) [Crystallogr. Rep. 54, 901 (2009)].

    Google Scholar 

  3. I. P. Kuranova, Mater. Struct. A 15(3), 43 (2008).

    Google Scholar 

  4. V. I. Timofeev, R. N. Chuprov-Netochin, V. R. Samigina, et al., Acta Ñrystallogr. F. 66, 259 (2009).

    Article  Google Scholar 

  5. V. I. Tishkov and V. O. Popov, Biokhimiya 69, 1537 (2004).

    Google Scholar 

  6. I. G. Shabalin, E. V. Filippova, K. M. Polyakov, et al., Acta Crystallogr. D 65, 1315 (2009).

    Article  Google Scholar 

  7. V. I. Tishkov and V. O. Popov, Biomol. Eng. 23, 89 (2006).

    Article  Google Scholar 

  8. D. C. Davison, Biochem. J. 49, 520 (1951).

    Google Scholar 

  9. C. Colas des Francs-Small, F. Ambard-Bretteville, A. Darpas, et al., Plant Physiol. 98, 273 (1992).

    Article  Google Scholar 

  10. C. Colas des Francs-Small, F. Ambard-Bretteville, I. D. Small, et al., Plant Physiol. 102, 1171 (1993).

    Article  Google Scholar 

  11. E. G. Sadykhov, A. E. Serov, I. E. Yasnyí, et al., Vestn. Mosk. Univ., Ser. 2: Khim. 47, 31 (2006).

    Google Scholar 

  12. D. Peacock and D. Boulter, Biochem. J. 120, 763 (1970).

    Google Scholar 

  13. V. O. Popov and V. S. Lamzin, Biochem. J. 301, 625 (1994).

    Google Scholar 

  14. I. G. Shabalin, T. V. Tikhonova, K. M. Polyakov, et al., EMBL Annual Rep. 2, 109 (2007).

    Google Scholar 

  15. B. W. Matthews, J. Mol. Biol. 33, 491 (1968).

    Article  Google Scholar 

  16. Z. Otwinowski and W. Minor, Methods Enzymol. 276, 307 (1997).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskiĭ pr. 59, Moscow, 119333, Russia

    I. G. Shabalin, V. I. Timofeev, V. R. Samygina & I. P. Kuranova

  2. Faculty of Chemistry, Moscow State University, Moscow, 119992, Russia

    A. E. Serov, O. E. Skirgello & V. I. Tishkov

  3. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskiĭ pr. 33, Moscow, 119071, Russia

    I. G. Shabalin & V. O. Popov

Authors
  1. I. G. Shabalin
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  2. A. E. Serov
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  3. O. E. Skirgello
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  4. V. I. Timofeev
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  5. V. R. Samygina
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  6. V. O. Popov
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  7. V. I. Tishkov
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  8. I. P. Kuranova
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Corresponding author

Correspondence to I. G. Shabalin.

Additional information

Original Russian Text © I.G. Shabalin, A.E. Serov, O.E. Skirgello, V.I. Timofeev, V.R. Samygina, V.O. Popov, V.I. Tishkov, I.P. Kuranova, 2010, published in Kristallografiya, 2010, Vol. 55, No. 5, pp. 855–859.

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Shabalin, I.G., Serov, A.E., Skirgello, O.E. et al. Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study. Crystallogr. Rep. 55, 806–810 (2010). https://doi.org/10.1134/S1063774510050159

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  • DOI: https://doi.org/10.1134/S1063774510050159

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