Abstract
The ability to produce extracellular laccase by strains of Azospirillum (A. brasilense Sp7, Sp107, Sp245, SR80, A. lipoferum SR42, Sp59b, A. picis TAR-3, A. tiophilum Bv-S, and A. amazonense Am) and Niveispirillum (N. irakense KBC1 and N. irakense KA3) has been established. Induction of enzyme activity by a number of aromatic compounds and copper ions has been shown. A homogeneous preparation of the extracellular laccase of A. brasilense Sp245 strain has been isolated and partially characterized for the first time.
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REFERENCES
Alexandre, G., Jacoud, C., and Faure, D., Population dynamics of a motile and a non-motile Azospirillum lipoferum strain during rice colonization and motility variation in the rhizosphere, FEMS Microbiol. Ecol., 1996, vol. 19, pp. 271–278.
Arora, D.S. and Gill, P.K., Production of ligninolytic enzymes by Phlebia floridensis, J. Microbiol. Biotechnol., 2005, no. 21, pp. 1021–1028.
Bradford, M.M., A rapid and sensitive method for the quantitation of microgram qualities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.
Castro-Sowinski, S., Martinez-Drets, G., and Okon, Y., Laccase activity in melanin-producing strains of Sinorhizobium meliloti, FEMS Microbiol. Lett., 2002, vol. 209, pp. 119–125.
Claus, H., Laccases and their occurrence in prokaryotes, Arch. Microbiol., 2003, vol. 179, pp. 145–150.
Diamantidis, G., Effosse, A., Potier, P., and Bally, R., Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum, Soil Biol. Biochem., 2000, vol. 32, pp. 919–927.
Edens, W.A., Going, T.Q., Dooley, D., and Henson, A.M., Characterization of a secreted laccase of Gaeumannomyces graminis var. tritici, Appl. Environ. Microbiol., 1999, vol. 65, no. 7, pp. 3071–3074.
Faraco, V., Giardina, P., Palmieri, G., and Sannia, G., Metal-activated laccase promoters, Prog. Biotechnol., 2002, vol. 21, pp. 105–111.
Givaudan, A., Effosse, A., Faure, D., Potier, P., Bouillant, M.-L., and Bally, R., Polyphenol oxidase in Azospirillum lipoferum isolated from rice rhizosphere: evidence for laccase activity in non-motile strains of Azospirillum lipoferum, FEMS Microbiol. Lett., 1993, vol. 108, pp. 205–210.
Koroleva, O.V., Stepanova, E.V., Gavrilova, V.P., Ikovleva, N.S., Landesman, E.O., Iavmetdinov, I.S., and Iaropolov, A.I., Laccase and Mn-peroxidase production by Coriolus lirgutus strain 075 in a jan fermentor, J. Biosci. Bioeng., 2002, vol. 93, no. 5, pp. 449–455.
Kuddus, M., Joseph, B., and Ramteke, P.W., Production of laccase from newly isolated Pseudomonas putida and its application in bioremediation of synthetic dyes and industrial effluents, Biocatal. Agricul. Biotechnol., 2013, vol. 2, pp. 333–338.
Laemmly, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.
Lin, S.Y., Hameed, A., Shen, F.T., Liu, Y.C., Hsu, Y.H., Shanina, M., Lai, W.A., and Young, C.C., Description of Niveispirillum fermenti gen. nov., sp. nov., isolated from a fermentor in Taiwan, transfer of Azospirillum irakense (1989) as Niveispirillum irakense comb. nov., and reclassification of Azospirillum amazonense (1983) as Nitrospirillum amazonense gen. nov., Antonie van Leeuwenhoek, 2014, vol. 105, pp. 1149–1162.
Malhotra, K., Sharma, P., and Capalash, N., Copper and dyes enhance laccase production in γ-proteobacterim JB, Biotechnol. Lett., 2004, vol. 26, pp. 1047–1050.
McMahon, M., Doyle, E.M., Brooks, S., and O’Connor, K.E., Biochemical characterisation of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6, Enzyme Microbial. Technol., 2007, vol. 40, pp. 1435–1441.
Mongkolthanaruk, W., Tongbopit, S., and Bhoonobtong, A., Independent behavior of bacterial laccases to inducers and metal ions during production and activity, Afr. J. Biotechnol., 2012, vol. 11, no. 39, pp. 9391–9398.
Morozova, O.V., Shumakovich, G.P., Gorbacheva, M.A., Shleev, S.V., and Yaropolov, A.I., “Blue” laccases, Biochemistry (Moscow), 2007, vol. 72, no. 10, pp. 1136–1150.
Muthukumarasamy, N.P., Jackson, B., Raj, J.A., and Sevanan, M., Production of extracellular laccase from Bacillus subtilis MTCC 2414 using agroresidues as a potential substrate, Biochem. Res. Int., 2015. Art. ID 765190.
Narayanan, P.M. and Murugan, S., Production, purification and application of bacterial laccase: a review, Biotechnology, 2014, vol. 13, no. 5, pp. 196–205.
Nikitina, V.E., Vetchinkina, E.P., Ponomareva, E.G., and Gogoleva, Yu.V., Phenol oxidase activity in bacteria of the genus Azospirillum, Microbiology (Moscow), 2010, vol. 79, no. 3, pp. 327–333.
Pozdnyakova, N.N., Turkovskaya, O.V., Yudina, E.N., and Rodakevich-Novak, Ya., Yellow laccase from the fungus Pleurotus ostreatus D1: purification and characterization, Appl. Biochem. Microbiol., 2006, vol. 42, no. 1, pp. 56–61.
Rajeswari, M. and Bhuvaneswari, V., Production of extracellular laccase from the newly isolated Bacillus sp. PK4, Afr. J. Biotechnol., 2016, vol. 15, pp. 1813–1826.
Sharma, P., Goel, R., and Capalash, N., Bacterial laccases, J. Microbiol. Biotechnol., 2007, vol. 23, pp. 823–832.
Shraddha Shekher, R., Sehgal, S., Kamthania, M., and Kumar, A., Laccase: microbial sources, production, purification, and potential biotechnological applications, Enzyme Res., 2011. Art. ID 217861.
Shutova, V.V., Revin, V.V., and Myakushina, Yu.A., The effect of copper ions on the production of laccase by the fungus Lentinus (Panus) tigrinus, Appl. Biochem. Microbiol., 2008, vol. 44, no. 6, pp. 619–623.
Vasconcelos, A.F.D., Barbosa, A.M., Dekker, R.F.H., Scarminio, I.S., and Rezende, M.I., Optimization of laccase production by Botryosphaeria sp. in the presence of veratryl alcohol by the response-surface method, Proc. Biochem., 2000, vol. 35, no. 10, pp. 1131–1138.
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Ponomareva, E.G., Kupryashina, M.A., Vetchinkina, E.P. et al. Extracellular Laccase Activity of Bacteria of the Genera Azospirillum and Niveispirillum. Biol Bull Russ Acad Sci 46, 161–167 (2019). https://doi.org/10.1134/S1062359019020134
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DOI: https://doi.org/10.1134/S1062359019020134