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A simple and rapid detection of viral protein using RNA oligonucleotide in a biosensor

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Abstract

The monitoring of non-structural viral protein 3 (NS3) has been of considerable interest in developing simple and reliable methods for detection of hepatitis C virus (HCV) for applications in diagnostic medicine. Although enzyme-linked immunosorbent assay (ELISA) is the most general method in HCV detection, using antibody brings problems. This method is temperature-sensitive and requires specific reactions condition. In addition, secondary antibody conjugated with enzyme and fluorescent dye is required. To overcome these bottlenecks, we designed a streptavidin-biotin conjugation method, namely, the RNA oligonucleotide sensor system that could monitor viral protein with detection limit of 500 pg/mL by using biotin-tagged RNA oligonucleotide in forteBio’s Octet optical biosensor system. In this study, we proposed an efficient method for simple and convenient detection of HCV viral protein, with the advantage of target specific monitoring.

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Authors and Affiliations

  1. Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 1266 Sinjeong-dong, Jeongeup, Jeonbuk, 580185, Republic of Korea

    Changhyun Roh & Sung-Kee Jo

  2. Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam, 463707, Republic of Korea

    Sang-Eun Kim

Authors
  1. Changhyun Roh
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  2. Sang-Eun Kim
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  3. Sung-Kee Jo
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Roh, C., Kim, SE. & Jo, SK. A simple and rapid detection of viral protein using RNA oligonucleotide in a biosensor. J Anal Chem 67, 925–929 (2012). https://doi.org/10.1134/S1061934812110044

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  • DOI: https://doi.org/10.1134/S1061934812110044

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