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Cytotoxic and photocytotoxic effect of Photofrin® on human laryngeal carcinoma (Hep2c) cell line

  • Laser Methods in Chemistry, Biology, and Medicine
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Laser Physics

Abstract

Significant apoptotic effect in Hep2c cell line has been investigated, when diode laser (λ = 635 nm, red) are used as a source of illumination and initiation of photodynamic action. The optimal uptake time of Photofrin® for Hep2c cell line was investigated by means of spectrophotometric measurement. Quantification of the live cell population was determined by means of neutral red assay (NRA). The spectrometry measurements showed that after 46 h incubation, the maximal cellular uptake of Photofrin® was achieved and photocytotoxic assay showed that light dose of 120 J/cm2 give effective PDT outcome for Hep2c cell line incubated with 85 μg/ml of Photofrin®. No significant phototoxic and cytotoxic effects on Hep2c cells were observed due to light doses or photosensitize, when studied independently of each other and Photofrin® showed good anti tumor effects.

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References

  1. H. Kato, J. Photochem. Photobiol. B: Biol. 42, 96 (1998).

    Article  Google Scholar 

  2. T. Okunaka, H. Kato, H. Tsutsui, T. Ishizumi, S. Ichinose, and Y. Kuroiwa, Lung Cancer 43, 77 (2004).

    Article  Google Scholar 

  3. T. G. Sutedja and P. E. Postmus, J. Photochem. Photobiol. B: Biol. 36, 199 (1996).

    Article  Google Scholar 

  4. H. Nakamura, N. Kawasaki, M. Hagiwara, A. Ogata, M. Saito, and C. Konaka, Lung Cancer 33, 51 (2001).

    Article  Google Scholar 

  5. J. L. Mulshine and F. R. Hirsch, Lung Cancer 41, 163 (2003).

    Article  Google Scholar 

  6. A. Khurshid, M. Atif, S. Firdous, S. Z. Z. Zaidi, R. Salman, and M. Ikram, Laser Phys. 20, 1673 (2010).

    Article  ADS  Google Scholar 

  7. J. L. Mulshine, Nat. Rev. Cancer 3, 65 (2003).

    Article  Google Scholar 

  8. S. H. Kreuzer, W. Schima, E. Schober, P. Pokieser, G. Kofler, and G. Lechner, Clinic. Radiol. 55, 775 (2000).

    Article  Google Scholar 

  9. A. Thakar, S. Bahadur, B. K. Mohanti, and S. Nivsarkar, J. Iaryngol. Otol. 114, 108 (2000).

    Google Scholar 

  10. H. Kato, M. Harada, S. Ichinose, J. Usuda, T. Tsuchida, and T. Okunaka, Photodiagn. Photodyn. Ther. 1, 49 (2004).

    Article  Google Scholar 

  11. Q. Peng, T. Warloe, J. Moan, H. Heyerdahl, H. B. Steen, J. M. Nesland, and K. E. Glercksky, Photochem. Photobiol. 62, 906 (1995).

    Article  Google Scholar 

  12. Z. Huang, Photodiagn. Photodyn. Ther. 3, 71 (2006).

    Article  Google Scholar 

  13. J. Usuda, H. Kato, T. Okunaka, K. Furukawa, H. Tsutsui, K. Yamada, Y. Suga, H. Honda, Y. Nagatsuka, T. Ohira, M. Tsuboi, and T. Hirano, J. Thorac. Oncol. 5, 489 (2006).

    Article  Google Scholar 

  14. K. Furuse, M. Fukuoka, and H. Kato, J. Clin. Oncol. 11, 1852 (1993).

    Google Scholar 

  15. A. Juarranz, P. Jaen, F. S. Rodriguez, J. Cuevas, and S. Gonzalez, Clinic. Transl. Oncol. 10, 148 (2008).

    Article  Google Scholar 

  16. O. Raab, Z. Biol. 1900, 524 (1900).

    Google Scholar 

  17. M. Atif, S. Firdous, A. Khurshid, L. Noreen, S. S. Z. Zaidi, and M. Ikram, Laser Phys. Lett. 6, 886 (2009).

    Article  Google Scholar 

  18. M. Atif, M. R. Stringer, J. E. C. Sawyer, P. E. Dyer, and S. B. Brown, Photodyn. Ther. 2, 235 (2005).

    Article  Google Scholar 

  19. A. Holder and D. L. Lagostera, 4OR 6, 167 (2008).

    Article  MATH  MathSciNet  Google Scholar 

  20. D. Nowis, M. Makowski, T. Stoklosa, M. Legat, T. Issat, and J. Golab, Acta Biochem. Polon. 52, 339 (2005).

    Google Scholar 

  21. D. I. Fielding, G. A. Buonaccorsi, and A. J. M. Robert, Chest 115, 502 (1999).

    Article  Google Scholar 

  22. A. F. Mironov, A. N. Nizhnik, and A. Y. Nockel, J. Photochem. Photobiol. 4, 297 (1990).

    Article  Google Scholar 

  23. T. J. Dougherty, Photochem. Photobiol. 46, 569 (1987).

    Article  Google Scholar 

  24. T. J. Dougherty, C. J. Gomer, and B. W. Henderson, J. Nat. Cancer Inst. 90, 889 (1998).

    Article  Google Scholar 

  25. M. Chen, A. Pennathur, and J. D. Luketich, Lasers Surg. Med. 38, 396 (2006).

    Article  Google Scholar 

  26. T. Okunaka, H. Kato, C. Konaka, K. Furukawa, M. Harada, and Y. Yamamoto, Diagnost. Therap. Endosc. 2, 203 (1996).

    Article  Google Scholar 

  27. J. Usuda, Y. Tsunoda, S. Ichinose, T. Ishizumi, K. Ohtani, S. Maehara, S. Ono, H. Tsutsui, T. Ohira, T. Okunaka, K. Furukawa, K. Y. Sugimoto, H. Kato, and N. Ikeda, Lung Cancer 67, 198 (2010).

    Article  Google Scholar 

  28. H. T. Whelan, M. H. Schmidt, A. D. Segura, T. L. McAuliffe, D. M. Bajic, K. J. Murray, J. E. Moulder, D. R. Strother, J. P. Thomas, and G. A. Meyer, J. Neurosurg. 79, 562 (1993).

    Article  Google Scholar 

  29. S. E. Jamel, Photodiagn. Photodyn. Ther. 7, 76 (2010).

    Article  Google Scholar 

  30. L. Y. Xue, S. M Chiu, and N. L. Oleinick, Exp. Cell Res. 263, 145 (2001).

    Article  Google Scholar 

  31. M. A. Biel, Laryngoscope 9, 1259–1268 (1998).

    Article  Google Scholar 

  32. K. Plaetzer, B. Krammer, J. Berlanda, F. Berr, and T. Kiesslich, Laser Med. Sci. 24, 259 (2009).

    Article  Google Scholar 

  33. L. Li, J. Xie, X. Zhang, J. Chen, Y. Luo, L. Zhang, and R. Luo, Photodiagn. Photodyn. Ther. 7, 139 (2010).

    Article  Google Scholar 

  34. R. R. Allison, G. H. Downie, X. H. Hu, C. J. H. Childs, R. Cuenca, and C. H. Sibata, Photodiagn. Photodyn. Ther. 1, 27 (2004).

    Article  Google Scholar 

  35. K. Chiba, K. Kawakami, and K. Tohyama, Toxicol. in Vitro. 12, 251 (1998).

    Article  Google Scholar 

  36. H. Babich and E. Borenfreund, Appl. Env. Microbiol. 57, 2101 (1991).

    Google Scholar 

  37. K. Moghissi and K. Dixon, Photodiagn. Photodyn. Ther. 5, 10 (2008).

    Article  Google Scholar 

  38. K. Moghissi, K. Dixon, J. A. C. Thorpe, C. Oxtoby, and M. R. Stringer, Photodiagn. Photodyn. Ther. 1, 253 (2004).

    Article  Google Scholar 

  39. Z. Huang, Cancer Res. Treatment 4, 283 (2005).

    Google Scholar 

  40. G. Jori, J. Photochem. Photobiol. B: Biol. 36, 87 (1996).

    Article  Google Scholar 

  41. M. Triesscheijn, P. Baas, J. H. Schellens, and F. A. Stewart, Oncologist. 11, 1034 (2006).

    Article  Google Scholar 

  42. S. Shrivastav, R. A. Bonar, K. R. Stone, and D. F. Panlson, Cancer Res. 40, 4438 (1980).

    Google Scholar 

  43. N. Tanigawa, D. H. Kern, Y. Hikasa, and D. L. Morton, Cancer Res. 42, 2159 (1982).

    Google Scholar 

  44. M. Atif, M., M. F. Alam, S. Firdous, S. S. Z. Zaidi, R. Suleman, and M. Ikram, Laser Phys. Lett. 7, 757 (2010).

    Article  ADS  Google Scholar 

  45. L. D. Prospero, G. Singh, B. C. Wilson, and A. J. Rainbow, Photochem. Photobiol. 38, 143 (1997).

    Article  Google Scholar 

  46. J. C. Lai, Cancer Biol. Therapy 5, 413 (2006).

    Article  Google Scholar 

  47. C. A. Robertson, D. H. Evans, and H. Abrahamse, J. Photochem. Photobiol. B: Biol. 96, 1 (2009).

    Article  Google Scholar 

  48. D. J. H. Roberts, F. Caimduff, I. Driver, B. Dixon, and S. B. Brown, J. Oncol. 5, 763 (1994).

    Google Scholar 

  49. S. A. Blan, J. F. Theumann, M. Forrer, G. Wagineres, H. V. D. Bergh, and P. H. Monnier, Laser Med. Sci. 12, 269 (1997).

    Article  Google Scholar 

  50. I. J. Macdonald and T. J. Dougherty, J. Porphyrins Phthalocyan. 5, 105 (2001).

    Article  Google Scholar 

  51. R. M. Szeimies, P. G. Cpinton, S. Karrer, B. Ortel, and M. Landthaler, J. Photochem. Photobiol. 36, 213 (1996).

    Article  Google Scholar 

  52. J. M. Runnels, N. Chen, B. Ortel, D. Kato, and T. Hasan, Brit. J. Cancer 80, 946 (1999).

    Article  Google Scholar 

  53. S. Kawauchi, Y. Morimoto, S. Sato, T. Arai, K. Seguchi, H. Asanuma, and M. Kikuchi, Lasers Med. Sci. 18, 179 (2003).

    Article  Google Scholar 

  54. H. Liang, D. S. Shin, and T. E. Lee, Laser Med. Sci. 15, 109 (2000).

    Article  Google Scholar 

  55. K. Kondo, T. Miyoshi, H. Fujino, H. Takizawa, S. Imai, N. Kobayashi, K. Kenzaki, S. Sakiyama, and A. Tangoku, Photodiagn. Photodyn. Ther. 4, 269 (2007).

    Article  Google Scholar 

  56. H. T. Whelan, M. H. Schmidt, and A. D. Segura, J. Neurol. 79, 562 (1993).

    Google Scholar 

  57. H. Ullah, M. Atif, S. Firdous, M. S. Mehmood, M. Ikram, C. Kurachi, C. Grecco, G. Nicolodelli, and V. S. Bagnato, Laser Phys. Lett. 7, 889 (2010).

    Article  Google Scholar 

  58. Y. Liu, P. Chen, F. Zhang, L. Lin, G.-Q. Tang, and G.-G. Mu, Laser Phys. Lett. 6, 465 (2009).

    Article  ADS  Google Scholar 

  59. R. S. Cavalcante, H. Imasato, V. S. Bagnato, and J. R. Perussi, Laser Phys. Lett. 6, 64 (2009).

    Article  ADS  Google Scholar 

  60. J. Ferreira, P. F. C. Menezes, C. Kurachi, C. H. Sibata, R. R. Allison, and V. S. Bagnato, Laser Phys. Lett. 4, 743 (2007).

    Article  ADS  Google Scholar 

  61. P. F. C. Menezes, V. S. Bagnato, R. M. Johnke, C. Bonnerup, C. H. Sibata, R. R. Allison, and J. R. Perussi, Laser Phys. Lett. 4, 546 (2007).

    Article  ADS  Google Scholar 

  62. D. Q. Bai, C. M. N. Yow, Y. Tan, E. S. M. Chu, and C. S. Xu, Laser Phys. 20, 544 (2010).

    Article  ADS  Google Scholar 

  63. J. Ferreira, P. F. C. Menezes, C. H. Sibata, R. R. Allison, S. Zucoloto, O. Castro e Silva, Jr., and V. S. Bagnato, Laser Phys. 19, 1932 (2009).

    Article  ADS  Google Scholar 

  64. P. F. C. Menezes, H. Imasato, V. S. Bagnato, C. H. Sibata, and J. R. Perussi, Laser Phys. 19, 1457 (2009).

    Article  ADS  Google Scholar 

  65. S. Pratavieira, P. L. A. Santos, P. F. C. Menezes, C. Kurachi, C. H. Sibata, M. T. Jarvi, B. C. Wilson, and V. S. Bagnato, Laser Phys. 19, 1263 (2009).

    Article  ADS  Google Scholar 

  66. Y. Y. Tian, L. L. Wang, and W. Wang, Laser Phys. 18, 1119 (2008).

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. National Institute of Laser and Optronics, Nilore, Islamabad, 45650, Pakistan

    M. Atif, S. Firdous & M. Nawaz

  2. Physics and Astronomy Department, College of Science, King Saud University, Riyadh, Saudi Arabia

    M. Atif

  3. Pakistan Institute of Engineering and Sciences, Nilore, Islamabad, 45650, Pakistan

    R. Mahmood, M. Fakhar-e-Alam & M. Ikram

  4. National Institute of Health, Islamabad, Pakistan

    S. S. Z. Zaidi & R. Suleman

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  1. M. Atif
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Original Text © Astro, Ltd., 2011.

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Atif, M., Firdous, S., Mahmood, R. et al. Cytotoxic and photocytotoxic effect of Photofrin® on human laryngeal carcinoma (Hep2c) cell line. Laser Phys. 21, 1235–1242 (2011). https://doi.org/10.1134/S1054660X11130020

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  • DOI: https://doi.org/10.1134/S1054660X11130020

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