Skip to main content
SpringerLink
Log in

Methodological approaches for studying apoplastic redox activity: 1. Mechanisms of peroxidase release

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

It was demonstrated the efficiency of the application of extracellular solution, i.e., the leachate derived from roots after their incubation, for the analysis of apoplastic redox enzymes (exemplified by peroxidase) in the roots of 5-day-old seedlings of spring wheat (Triticum aestivum L., cv. Kazanskaya yubileinaya) after wounding stress. Such non-invasive approach allows the studying of enzymes without a disruption of plant tissue. The inhibitory analysis showed that newly synthesized and pre-existing in the cells peroxidases were not released from the cytoplasm at stress conditions. The proposed methodological approach for obtaining the enzymatic extracts of apoplastic redox enzymes opens up broad prospects for researches of plant immunity and stress responses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AD:

actinomycin D

BFA:

brefeldin A

CH:

cycloheximide

ECS:

extracellular solution

G6PDH:

glucose-6-phosphate dehydrogenase

PIB:

post-infiltration buffer

References

  1. Dumas, B., Bottin, A., Gaulin, E., and Esquerré-Tugayé, M.T., Cellulose-binding domains: cellulose associated-defensive sensing partners? Trends Plant Sci., 2008, vol. 13, pp. 160–164.

    Article  CAS  PubMed  Google Scholar 

  2. Eller, A.S. and Sparks, J.P., Predicting leaf-level fluxes of O3 and NO2: the relative roles of diffusion and biochemical processes, Plant Cell Environ., 2006, vol. 29, pp. 1742–1750.

    Article  CAS  PubMed  Google Scholar 

  3. Sharova, E.I., Bilova, T.E., and Medvedev, S.S., Axial changes in apoplast properties in the elongation zone of maize mesocotyl, Russ. J. Plant Physiol., 2012, vol. 59, pp. 565–572.

    Article  CAS  Google Scholar 

  4. Gordon, L.Kh., Functional characteristic of adaptive senescence of excised wheat roots, Fiziol. Biokh. Kul’t. Rast., 1992, vol. 24, pp. 128–133.

    Google Scholar 

  5. Ivanov, V.B., Using the roots as test objects for the assessment of biological action of chemical substances, Russ. J. Plant Physiol., 2011, vol. 58, pp. 1082–1090.

    Article  CAS  Google Scholar 

  6. Sobell, H.M., Actinomycin and DNA transcription, Proc. Natl. Acad. Sci. USA, 1985, vol. 82, pp. 5328–5331.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Obrig, T.G., Culp, W.J., McKeehan, W.L., and Hardesty, B., The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes, J. Biol. Chem., 1971, vol. 246, pp. 174–181.

    CAS  PubMed  Google Scholar 

  8. Satiat-Jeunemaitre, B. and Hawes, C., Redistribution of a Golgi glycoprotein in plant cells treated with brefeldin A, J. Cell Sci., 1992, vol. 103, pp. 1153–1166.

    CAS  Google Scholar 

  9. Pimpl, P., Movafeghi, A., Coughlan, S., Denecke, J., Hillmer, S., and Robinson, D.G., In situ localization and in vitro induction of plant COPI-coated vesicles, Plant Cell, 2000, vol. 12, pp. 2219–2235.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Nebenfuhr, A., Ritzenthaler, C., and Robinson, D.G., Brefeldin A: deciphering an enigmatic inhibitor of secretion, Plant Physiol., 2002, vol. 130, pp. 1102–1108.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Minibayeva, F., Kolesnikov, O., Chasov, A., Beckett, R.P., Lüthje, S., Vylegzhanina, N., Buck, F., and Böttger, M., Wound-induced apoplastic peroxidase activities: their roles in the production and detoxification of reactive oxygen species, Plant Cell Environ., 2009, vol. 32, pp. 497–508.

    Article  CAS  PubMed  Google Scholar 

  12. Chasov, A.V., Kolesnikov, O.P., Minibayeva, F.V., and Gordon, L.Kh., Monensin and cycloheximide do not inhibit peroxidase release and superoxide ion production in wheat roots under wounding stress, Vestn. Kharkovsk. Nats. Agrarn. Univ., Ser. B, 2005, no. 2 (7), pp. 29–34.

    Google Scholar 

  13. Chasov, A.V. and Minibayeva, F.V., Effect of exogenous phenols on superoxide production by extracellular peroxidase from wheat seedling roots, Biochemistry (Moscow), 2009, vol. 74, pp. 766–774.

    CAS  PubMed  Google Scholar 

  14. Kochetov, G.A., Prakticheskoe rukovodstvo po enzimologii (Practical Manual for Enzymology), Moscow: Vyssh. shk., 1980.

    Google Scholar 

  15. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  16. Bradford, M.M., A rapid and sensitive method for the quantitations of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  CAS  PubMed  Google Scholar 

  17. Chasov, A.V. and Minibayeva, F.V., Methodological Approaches for Studying Apoplastic Redox Activity: 2. Regulation of Peroxidase Activity, Russ. J. Plant Physiol., 2014, vol. 61 (in press).

  18. Chasov, A.V., Gordon, L.Kh., Kolesnikov, O.P., and Minibayeva, F.V., Cell surface peroxidase is a generator of superoxide anion in wounded wheat root cells, Tsitologiya, 2002, vol. 44, pp. 691–696.

    CAS  Google Scholar 

  19. Maksimov, I.V., Sorokan’, A.V., Cherepanova, E.A., Surina, O.B., Troshina, N.B., and Yarullina, L.G., Effects of salicylic and jasmonic acids on the components of pro-/antioxidant system in potato plants infected with late blight, Russ. J. Plant Physiol., 2011, vol. 58, pp. 299–306.

    Article  CAS  Google Scholar 

  20. Hiraga, S., Sasaki, K., Ito, H., Ohashi, Y., and Matsui, H., A large family of class III plant peroxidases, Plant Cell Physiol., 2001, vol. 42, pp. 462–468.

    Article  CAS  PubMed  Google Scholar 

  21. Levitskii, D.O. and Lebedev, A.V., Antibiotics in the lipid bilayer: carriers and channel-forming proteins (polyether antibiotics), Itogi Nauki i Tekhniki, Ser. Biophysics, 1988, vol. 23.

  22. Robineau, S., Chabre, M., and Antonny, B., Binding site of brefeldin A at the interface between the small G protein ADP-ribosylation factor 1 (ARF1) and the nucleotide-exchange Factor Sec7 domain, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 9913–9918.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Almagro, L., Gómez, Ros, L.V., Belchi-Navarro, S., Bru, R., Ros, Barceló, A., and Pedreño, M.A., Class III peroxidases in plant defence reactions, J. Exp. Bot., 2009, vol. 60, pp. 377–390.

    Article  CAS  PubMed  Google Scholar 

  24. Nair, A.R. and Showalter, A.M., Purification and characterization of a wound-inducible cell wall cationic peroxidase from carrot roots, Biochem. Biophys. Res. Commun., 1996, vol. 226, pp. 254–260.

    Article  CAS  PubMed  Google Scholar 

  25. Alekseeva, V.Ya., Gordon, L.Kh., Kolesnikov, O.P., and Tsentsevitskii, A.N., The influence of plasma membrane ion permeability modulators on superoxide formation and bioelectrical characteristics of wheat root cells, Tsitologiya, 2005, vol. 47, pp. 578–583.

    CAS  Google Scholar 

  26. Schützendübel, A. and Polle, A., Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization, J. Exp. Bot., 2002, vol. 53, pp. 1351–1365.

    Article  PubMed  Google Scholar 

  27. Fang, W.C. and Kao, C.H., Enhanced peroxidase activity in rice leaves in response to excess iron, copper and zinc, Plant Sci., 2000, vol. 158, pp. 71–76.

    Article  CAS  PubMed  Google Scholar 

  28. Lukoyanova, M.A., The role of noncovalent interactions in membrane stabilization, Mekhanizmy pogloshcheniya veshchestv rastitel’noi kletkoi (Mechanisms of Substance Uptake by Plant Cell), Salyaev, R.K., Ed., Irkutsk, 1971, pp. 24–30.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, ul. Lobachevskogo 2/31, Kazan, 420111, Russia

    A. V. Chasov & F. V. Minibayeva

Authors
  1. A. V. Chasov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. V. Minibayeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Chasov.

Additional information

Original Russian Text © A.V. Chasov, F.V. Minibayeva, 2014, published in Fiziologiya Rastenii, 2014, Vol. 61, No. 4, pp. 594–602.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chasov, A.V., Minibayeva, F.V. Methodological approaches for studying apoplastic redox activity: 1. Mechanisms of peroxidase release. Russ J Plant Physiol 61, 556–563 (2014). https://doi.org/10.1134/S1021443714040049

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443714040049

Keywords

Search

Navigation