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Protection of growth and photosynthesis of Brassica juncea genotype with dual type sulfur transport system against sulfur deprivation by coordinate changes in the activities of sulfur metabolism enzymes and cysteine and glutathione production

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Abstract

Mustard (Brassica juncea L. Czern and Coss.) cvs. Pusa Jai Kisan (with low-affinity S transporter (LAT) system) and Pusa Bold (with dual, low- and high-affinity transporters (LAT + HAT) system) were supplied with 0 or 1 mM S in hydroponics culture, and the coordinate changes in growth traits (plant dry weight and leaf area), photosynthetic traits (photosynthetic rate, intercellular CO2, F v/F m, and chlorophyll content), activities of key enzymes of sulfur metabolism, such as ATP-sulfurylase (ATP-S), serine acetyltransferase (SAT), and glutathione reductase (GR), and the contents of cysteine (Cys) and glutathione (GSH) were studied in 30 days after sowing. The results showed that cv. Pusa Jai Kisan was more sensitive to S deprivation than cv. Pusa Bold. In cv. Pusa Jai Kisan, S deprivation resulted in a stronger decrease of plant growth and photosynthetic traits, Cys and GSH contents, and a notable decline in activity of ATP-S. S deprivation up-regulated GR activity to a greater extent in cv. Pusa Bold. In contrast, despite the activity of SAT, an enzyme involved in the final step of Cys biosynthesis, was increased in cv. Pusa Jai Kisan stronger than in cv. Pusa Bold under S-deprivation, it could not be translated into the increase in Cys and, thus, GSH contents and a consequent improvement in growth and photosynthesis. The study demonstrated that cv. Pusa Bold (with LAT + HAT) can be a promising cultivar for activation of Cys and/or GSH biosyntheses and increased plant tolerance to S-deprivation conditions.

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Abbreviations

ATP-S:

ATP-sulfurylase

Chl:

chlorophyll

DTNB:

dithiobis-2-nitrobenzoic acid

GSH:

glutathione (reduced)

HAT:

high-affinity transporter

LAT:

low-affinity transporter

OAS:

o-acetylserine

SAT:

serine-acetyltransferase

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Authors and Affiliations

  1. Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India

    N. A. Anjum & N. A. Khan

  2. Department of Botany, Faculty of Science, Hamdard University, New Delhi, 110062, India

    S. Umar & M. Iqbal

  3. CESAM and Department of Chemistry, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal

    N. A. Anjum, I. Ahmad & M. E. Pereira

  4. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Post Box, 2460, Riyadh, 11451, Saudi Arabia

    M. Iqbal

Authors
  1. N. A. Anjum
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  2. S. Umar
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  3. M. Iqbal
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  4. I. Ahmad
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  5. M. E. Pereira
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  6. N. A. Khan
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Correspondence to N. A. Anjum.

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Anjum, N.A., Umar, S., Iqbal, M. et al. Protection of growth and photosynthesis of Brassica juncea genotype with dual type sulfur transport system against sulfur deprivation by coordinate changes in the activities of sulfur metabolism enzymes and cysteine and glutathione production. Russ J Plant Physiol 58, 892–898 (2011). https://doi.org/10.1134/S1021443711050025

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