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Regulation of cytokinin oxidase activity as a factor affecting the content of cytokinins

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Abstract

In the shoots of 7-day-old seedlings of wheat (Triticum durum Desf., cv. Bezenchukskaya 139), ion deficiency in Hoagland-Arnon nutrient solution induced a decrease in the cytokinin content by the end of the first day, whereas the excision of four out of five primary roots brought about an opposite response (accumulation of cytokinins) as early as during the first hour. It was assumed that changes in the content of cytokinins might depend on the activity of cytokinin oxidase (CKO) and the expression of gene encoding this enzyme. It was shown that tenfold dilution of the nutrient solution activated CKO and raised the level of CKO gene expression, whereas excision of some roots brought about a quick decrease in enzyme activity and gene expression. The role of ABA and arrival of cytokinins from the roots to the shoot as factors affecting CKO activity in the shoot is discussed; arguments for the priority of hormonal signal over the influx of nitrates from the roots are offered. It was concluded that the regulation of CKO activity might be one of the important mechanisms determining plant response to treatments via the changes in the cytokinin concentration.

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Abbreviations

CKO:

cytokinin oxidase

H-A:

Hoagland-Arnon nutrient solution

IPA:

isonentenyladenosine

RCW:

relative content of water

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Authors and Affiliations

  1. Institute of Biology, Ufa Research Center, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, 450054, Russia

    L. B. Vysotskaya & G. R. Kudoyarova

  2. Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, Ufa, Russia

    A. M. Aval’baev, R. A. Yuldashev & F. M. Shakirova

  3. Bashkir State University, Ufa, Russia

    S. Yu. Veselov

Authors
  1. L. B. Vysotskaya
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  2. A. M. Aval’baev
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  3. R. A. Yuldashev
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  4. F. M. Shakirova
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  5. S. Yu. Veselov
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  6. G. R. Kudoyarova
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Correspondence to L. B. Vysotskaya.

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Original Russian Text © L.B. Vysotskaya, A.M. Aval’baev, R.A. Yuldashev, F.M. Shakirova, S.Yu. Veselov, G.R. Kudoyarova, 2010, published in Fiziologiya Rastenii, 2010, Vol. 57, No.4, pp. 530–537.

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Vysotskaya, L.B., Aval’baev, A.M., Yuldashev, R.A. et al. Regulation of cytokinin oxidase activity as a factor affecting the content of cytokinins. Russ J Plant Physiol 57, 494–500 (2010). https://doi.org/10.1134/S1021443710040060

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  • DOI: https://doi.org/10.1134/S1021443710040060

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