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Accelerated root growth induced by nitrate deficit is related to apoplast acidification

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Abstract

We measured the rate of growth, osmotic pressure, hydraulic conductance, longitudinal and transverse extensibility of barley (Hordeum vulgare L.) roots in Knop solution with nitrate and at substitution of NO −3 with Cl−. During the first three days after NO −3 removal, root growth acceleration was related to the increase in their longitudinal extensibility. It was shown that root exposure to buffer with pH 4.5 and also activation of H+ pump with naphthyl acetate imitated changes in extensibility induced by NO −3 deficit. Earlier, we have demonstrated medium acidification near root surface and calculated its expected level (pH 4.5). This permits a supposition that the cause for changes in extensibility and root growth acceleration at NO −3 deficit was apoplast acidification, evidently related to the ceasing of NO −3 symport with H+ and activation of the plasmalemmal H+ pump. ABA did not affect root extensibility at pH 4.5; however, at pH 6.0, it was similar to the action of diethylstilbestrol, an inhibitor of H+ pump, and opposite to the action of NO −3 deficit. Thus, the absence of ABA effects on root growth, in spite of its accumulation at NO −3 deficit, could be explained by apoplast acidification as well.

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Abbreviations

cMF:

cellulose microfibrils

cMT:

cortical microtubules

D :

root diameter

δD/D :

transverse root extensibility

DES:

diethylstilbestrol

δl :

longitudinal root extensibility

L p :

hydraulic conductance of rhizodermis membranes

Π:

intracellular osmotic pressure

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Authors and Affiliations

  1. Agrophysical Research Institute, Russian Academy of Agricultural Sciences, Grazhdanskii pr. 14, St. Petersburg, 195220, Russia

    O. V. Skobeleva, I. N. Ktitorova & K. G. Agal’tsov

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  1. O. V. Skobeleva
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  2. I. N. Ktitorova
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  3. K. G. Agal’tsov
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Correspondence to O. V. Skobeleva.

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Original Russian Text © O.V. Skobeleva, I N. Ktitorova, K.G. Agal’tsov, 2010, published in Fiziologiya Rastenii, 2010, Vol. 57, No.4, pp. 520–529.

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Skobeleva, O.V., Ktitorova, I.N. & Agal’tsov, K.G. Accelerated root growth induced by nitrate deficit is related to apoplast acidification. Russ J Plant Physiol 57, 485–493 (2010). https://doi.org/10.1134/S1021443710040059

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