Skip to main content
SpringerLink
Log in

Antioxidant enzymes and physiological characteristics in two Jerusalem artichoke cultivars under salt stress

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

An Erratum to this article was published on 08 January 2009

Abstract

The effects of NaCl stress on the activity of antioxidant enzymes, lipid peroxidation, cell membrane stability, net photosynthetic rate, gas-exchange, and chlorophyll content were investigated in two Jerusalem artichoke cultivars, Dafeng (salt-tolerant) and Wuxi (salt-sensitive), grown under control (nutrient solution) or salt stress (nutrient solution containing 75, 150, and 225 mM NaCl) conditions for 7 days. In leaves of salt-tolerant cv. Dafeng, superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), and catalase (EC 1.11.1.6) activities significantly increased as compared to the controls, whereas no significant change was observed in cv. Wuxi. Lipid peroxidation and cell membrane injury were enhanced in both cultivars. Net photosynthesis and stomatal conductance decreased in response to salt stress, but cv. Dafeng showed a smaller reduction in photosynthesis than cv. Wuxi. The results indicated that stomatal aperture limited leaf photosynthetic capacity in the NaCl-treated plants of both cultivars. However, significant reduction in the leaf chlorophyll content due to NaCl stress was observed only in cv. Wuxi. These results suggested that salt-tolerant Jerusalem artichoke varieties may have a better protection against reactive oxygen species, at least in part, by increasing the activity of antioxidant enzymes under salt stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CAT:

catalase

MDA:

malondialdehyde

NBT:

nitroblue tetrazolium

POD:

peroxidase

ROS:

reactive oxygen species

SOD:

superoxide dismutase

References

  1. Zhu, J.K., Hasegawa, P.M., and Bressan, R.A., Molecular Aspects of Osmotic Stress in Plants, Crit. Rev. Plant Sci., 1997, vol. 16, pp. 253–277.

    Article  CAS  Google Scholar 

  2. Serrano, R., Mulet, J.M., Rios, G., Marquez, J.A., de Larriona, I.F., Leube, M.P., Mendizabal, I., Pascual-Ahuir, A., Proft, M.R.R., and Montesinos, C., A Glimpse of the Mechanism of Ion Homeostasis during Salt Stress, J. Exp. Bot., 1999, vol. 50, pp. 1023–1036.

    Article  CAS  Google Scholar 

  3. Rodriguez-Navarro, A., Potassium Transport in Fungi and Plants, Biochim. Biophys. Acta, 2000, vol. 1469, pp. 1–30.

    CAS  PubMed  Google Scholar 

  4. Zhu, J.K., Regulation of Ion Homeostasis under Salt Stress, Curr. Opin. Plant Biol., 2003, vol. 6, pp. 441–445.

    Article  CAS  PubMed  Google Scholar 

  5. Mittler, R., Oxidative Stress, Antioxidants and Stress Tolerance, Trends Plant Sci., 2002, vol. 7, pp. 405–410.

    Article  CAS  PubMed  Google Scholar 

  6. Neill, S., Desikan, R., and Hancock, J., Hydrogen Peroxide Signaling, Curr. Opin. Plant Biol., 2002, vol. 5, pp. 388–395.

    Article  CAS  PubMed  Google Scholar 

  7. Imlay, J.A., Pathways of Oxidative Damage, Annu. Rev. Microbiol., 2003, vol. 57, pp. 395–418.

    Article  CAS  PubMed  Google Scholar 

  8. Rout, N.P. and Shaw, B.P., Salt Tolerance in Aquatic Macrophytes: Possible Involvement of the Antioxidative Enzymes, Plant Sci., 2001, vol. 160, pp. 415–423.

    Article  CAS  PubMed  Google Scholar 

  9. Hernández, J.A., Jiménez, A., Mullineaux, P., and Sevilla, F., Tolerance of Pea (Pisum sativum L.) to Long-Term Salt Stress Is Associated with Induction of Antioxidant Defenses, Plant Cell Environ., 2000, vol. 23, pp. 853–862.

    Article  Google Scholar 

  10. Mittova, V., Tal, M., Volokita, M., and Guy, M., Salt Stress Induces Up-Regulation of an Efficient Chloroplast Antioxidant System in the Salt Tolerant Wild Tomato Species Lycopersicon pennellii But Not in the Cultivated Species, Physiol. Plant., 2002, vol. 115, pp. 393–400.

    Article  CAS  PubMed  Google Scholar 

  11. De Azevedi, Neto, A.D., Prisco, J.T., Eněas-Filho, J., de Abreu, C.E.B., and Gomes-Filho, E., Effect of Salt Stress on Antioxidative Enzymes and Lipid Peroxidation in Leaves and Roots of Salt-Tolerant and Salt-Sensitive Maize Genotypes, Environ. Exp. Bot., 2006, vol. 56, pp. 87–94.

    Article  Google Scholar 

  12. Noctor, G. and Foyer, C.H., Ascorbate and Glutathione: Keeping Active Oxygen under Control, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1998, vol. 49, pp. 249–279.

    Article  CAS  PubMed  Google Scholar 

  13. Hernández, J.A. and Almansa, M.S., Short-Term Effects of Salt Stress on Antioxidant Systems and Leaf Water Relations of Pea Leaves, Physiol. Plant., 2002, vol. 115, pp. 251–257.

    Article  PubMed  Google Scholar 

  14. Meloni, D.A., Oliva, M.A., Martinez, C.A., and Cambraia, J., Photosynthesis and Activity of Superoxide Dismutase, Peroxidase and Glutathione Reductase in Cotton under Salt Stress, Environ. Exp. Bot., 2003, vol. 49, pp. 69–76.

    Article  CAS  Google Scholar 

  15. Monti, A., Amaducci, M.T., and Venturi, G., Growth Response, Leaf Gas Exchange and Fructans Accumulation of Jerusalem Artichoke (Helianthus tuberosus L.) as Affected by Different Water Regimes, Eur. J. Agron., 2005, vol. 23, pp. 136–145.

    Article  CAS  Google Scholar 

  16. Saengthongpinit, W. and Sajjaanantakul, T., Influence of Harvest Time and Storage Temperature on Characteristics of Inulin from Jerusalem Artichoke (Helianthus tuberosus L.) Tubers, Postharvest Biol. Technol., 2005, vol. 37, pp. 93–100.

    Article  CAS  Google Scholar 

  17. Liu, Z.P., Deng, L.Q., Liu, L., Qi, C.H., Chen, M.D., and Xia, T.X., Physiological Characteristics of Helianthus tuberosus L. Irrigated by Seawater, Laizhou, Shandong Province, J. Plant Ecol., 2005, vol. 29, pp. 474–478.

    CAS  Google Scholar 

  18. Wellburn, A.R., The Spectral Determination of Chlorophylls a and b, as Well as Total Carotenoids Using Various Solvents with Spectrophotometers of Different Resolution, J. Plant Physiol., 1994, vol. 144, pp. 307–313.

    CAS  Google Scholar 

  19. Zhao, H.J. and Tan, J.F., Role of Calcium Ion in Protection against Heat and High Irradiance Stress-Induced Oxidative Damage to Photosynthesis of Wheat Leaves, Photosynthetica, 2005, vol. 43, pp. 473–476.

    Article  CAS  Google Scholar 

  20. Lutts, S., Kiner, J.M., and Bouharmont, J., NaCl-Induced Senescence in Leaves of Rice (Oryza sativa L.) Cultivars Differing in Salinity Resistance, Ann. Bot., 1996, vol. 78, pp. 389–398.

    Article  CAS  Google Scholar 

  21. Chen, L.Z., Wang, W.Q., and Lin, P., Photosynthetic and Physiological Responses of Kandelia candel L. Druce Seedlings to Duration of Tidal Immersion in Artificial Seawater, Environ. Exp. Bot., 2005, vol. 54, pp. 256–266.

    Article  CAS  Google Scholar 

  22. Ghazi, H.B., Yasuo, Y., Emi, S., Ryozo, S., Naoyoshi, K., Kunisuke, T., and Kiyoshi, T., Enhanced Tolerance to Salt Stress and Water Deficit by Overexpressing Superoxide Dismutase in Tobacco (Nicotiana tabacum) Chloroplasts, Plant Sci., 2004, vol. 166, pp. 919–928.

    Article  Google Scholar 

  23. Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Using the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  CAS  PubMed  Google Scholar 

  24. Polle, A., Dissecting the Superoxide Dismutase-Ascorbate Peroxidase-Glutathione Pathway in Chloroplasts by Metabolic Modeling. Computer Simulation as a Step towards Flux Analysis, Plant Physiol., 2001, vol. 126, pp. 445–462.

    Article  CAS  PubMed  Google Scholar 

  25. Meneguzzo, S., Navarri-Izzo, F., and Izzo, R., Antioxidative Responses of Shoots and Roots of Wheat to Increasing NaCl Concentrations, J. Plant Physiol., 1999, vol. 155, pp. 274–280.

    CAS  Google Scholar 

  26. Yu, Q. and Rengel, Z., Drought and Salinity Differentially Influence Activities of Superoxide Dismutases in Narrow-Leafed Lupins, Plant Sci., 1999, vol. 142, pp. 1–11.

    Article  CAS  Google Scholar 

  27. Jahnke, L.S. and White, A.L., Long-Term Hyposaline and Hypersaline Stresses Produce Distinct Antioxidant Responses in the Marine Alga Dunaliella tertiolecta, J. Plant Physiol., 2003, vol. 160, pp. 1193–1202.

    Article  CAS  PubMed  Google Scholar 

  28. Sudhakar, C., Lakshmi, A., and Giridarakumar, S., Changes in the Antioxidant Enzyme Efficacy in Two High Yielding Genotypes of Mulberry (Morus alba L.) under NaCl Salinity, Plant Sci., 2001, vol. 161, pp. 613–619.

    Article  CAS  Google Scholar 

  29. Liang, Y., Chen, Q., Liu, Q., Zhang, W., and Ding, R., Exogenous Silicon (Si) Increases Antioxidant Enzyme Activity and Reduces Lipid Peroxidation in Roots of Salt-Stressed Barley (Hordeum vulgare L.), J. Plant Physiol., 2003, vol. 160, pp. 1157–1164.

    Article  CAS  PubMed  Google Scholar 

  30. Steduto, P., Albrizio, R., Giorio, P., and Sorrentino, G., Gas Exchange Response and Stomatal and Non-Stomatal Limitations to Carbon Assimilation of Sunflower under Salinity, Environ. Exp. Bot., 2000, vol. 44, pp. 243–255.

    Article  PubMed  Google Scholar 

  31. Sultana, N., Ikeda, T., and Itoh, R., Effect of NaCl Salinity on Photosynthesis and Dry Matter Accumulation in Developing Rice Grains, Environ. Exp. Bot., 1999, vol. 42, pp. 211–220.

    Article  CAS  Google Scholar 

  32. Delfine, S., Alvino, A., Villani, M.C., and Loreto, F., Restrictions to Carbon Dioxide Conductance and Photosynthesis in Spinach Leaves Recovering from Salt Stress, Plant Physiol., 1999, vol. 119, pp. 1101–1106.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Y. F. Xue & Zh. P. Liu

  2. State Key Laboratory Breeding Base, Key Lab of Food quality and Safety of Jangsu Province, Nanjing, 210014, China

    Y. F. Xue & Zh. P. Liu

Authors
  1. Y. F. Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zh. P. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. F. Xue.

Additional information

Original Russian Text © Y.F. Xue, Zh.P. Liu, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 6, pp. 863–858.

This text was submitted by the authors in English.

An erratum to this article can be found online at http://dx.doi.org/10.1134/S1021443709010221.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xue, Y.F., Liu, Z.P. Antioxidant enzymes and physiological characteristics in two Jerusalem artichoke cultivars under salt stress. Russ J Plant Physiol 55, 776–781 (2008). https://doi.org/10.1134/S102144370806006X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S102144370806006X

Key words

Search

Navigation