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Virus-induced gene silencing in plant species

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Abstract

As a method of post-transcriptional gene silencing (PTGS), virus-induced gene silencing (VIGS) became a powerful tool for the study of gene function in plants. Current researches are focused on unraveling the mechanisms of PTGS, finding novel viral vectors and new infection methods in order to broaden the host species spectrum, and establishing the new reporter gene system of VIGS to demonstrate gene silencing, as well as developing the high-throughput silencing for genomic scale research. The aim of this review is to bring together recent advances of VIGS, an attractive method, for understanding it in the study of plants.

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Abbreviations

dsRNA:

double-stranded RNA

GFP:

green fluorescent protein gene

PDS:

phytoene desaturase gene

PTGS:

post-transcriptional gene silencing

RDRP:

RNA-dependent RNA polymerase

RNAi:

RNA interference

TRV:

tobacco rattle virus

VIGS:

virus-induced gene silencing

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Authors and Affiliations

  1. Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University, PO Box 204, No. 17 Qinghua East Road, Haidian, Beijing, 100083, China

    Y. Shao, H. L. Zhu, H. Q. Tian, X. G. Wang, X. J. Lin, B. Z. Zhu, Y. H. Xie & Y. B. Luo

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  1. Y. Shao
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  2. H. L. Zhu
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  3. H. Q. Tian
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Correspondence to Y. B. Luo.

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Shao, Y., Zhu, H.L., Tian, H.Q. et al. Virus-induced gene silencing in plant species. Russ J Plant Physiol 55, 168–174 (2008). https://doi.org/10.1134/S1021443708020027

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  • DOI: https://doi.org/10.1134/S1021443708020027

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