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Hydration-dehydration of human serum albumin studied by isothermal calorimetry and IR spectroscopy

  • Biophysical Chemistry
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Abstract

Based on a comparison of the data on the isothermal calorimetry of the interaction of human serum albumin with water and the adsorption isotherms of water vapor on the protein obtained by IR spectroscopy, an experimental method was used for the first time to study the thermochemical and sorption characteristics of protein hydration-dehydration over the entire range of the thermodynamic activities of water. A mechanism was proposed to explain the relationships between the thermochemical properties, protein water content, and the moistening method.

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Authors and Affiliations

  1. Butlerov Institute of Chemistry, Kazan State University, Kazan, 420008, Tatarstan, Russia

    V. A. Sirotkin, D. V. Korolev & A. E. Silakova

Authors
  1. V. A. Sirotkin
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  2. D. V. Korolev
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  3. A. E. Silakova
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Additional information

Original Russian Text © V.A. Sirotkin, D.V. Korolev, A.E. Silakova, 2007, published in Zhurnal Fizicheskoi Khimii, 2007, Vol. 81, No. 8, pp. 1518–1523.

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Sirotkin, V.A., Korolev, D.V. & Silakova, A.E. Hydration-dehydration of human serum albumin studied by isothermal calorimetry and IR spectroscopy. Russ. J. Phys. Chem. 81, 1341–1345 (2007). https://doi.org/10.1134/S0036024407080298

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  • DOI: https://doi.org/10.1134/S0036024407080298

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