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Structural Peculiarities and Biological Properties of the Lipopolysaccharide from Herbaspirillum seropedicae Z78

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Abstract—

Lipopolysaccharide was isolated by phenol extraction from the surface membrane of the nitrogen-fixing endophytic rhizobacterium Herbaspirillum seropedicae, strain Z78. The lipopolysaccharide’s lipid A contained 3-hydroxydecanoic, 3-hydroxydodecanoic, dodecanoic, tetradecanoic, and hexadecanoic acids. The 3-hydroxydodecanoic acid was amide-linked to the sugar backbone of the lipid A. The structure of the O-polysaccharide from H. seropedicae Z78 was established for the first time. It is characterized by heterogeneity and by the presence of glycerol, a component rarely found in gram-negative bacteria. The O polysaccharide of H. seropedicae Z78 was found to consist of two types of repeating units: one represented by glycerol-1-phosphate and the other by the glycerol-1-phosphate of the backbone, which is substituted at the 2-position by N-acetyl-D-glucosamine. The lipopolysaccharide of the H. seropedicae Z78 was weakly toxic to warm-blooded animals and moderately and dose-dependently induced interleukin synthesis by human whole blood cells and NO synthesis by mouse splenocytes. This may indicate that the H. seropedicae lipopolysaccharide is a promising antagonist of classical endotoxins.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia

    N. S. Velichko, A. K. Surkina, Y. P. Fedonenko & S. A. Konnova

  2. Chernyshevsky Saratov State University, Saratov, Russia

    Y. P. Fedonenko & S. A. Konnova

  3. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

    E. L. Zdorovenko

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  1. N. S. Velichko
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  2. A. K. Surkina
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  3. Y. P. Fedonenko
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  4. E. L. Zdorovenko
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  5. S. A. Konnova
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Correspondence to N. S. Velichko.

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Translated by E. Dedyukhina

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Velichko, N.S., Surkina, A.K., Fedonenko, Y.P. et al. Structural Peculiarities and Biological Properties of the Lipopolysaccharide from Herbaspirillum seropedicae Z78. Microbiology 87, 635–641 (2018). https://doi.org/10.1134/S002626171805017X

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  • DOI: https://doi.org/10.1134/S002626171805017X

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