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Comparative analysis of biodiversity in the planktonic and biofilm bacterial communities in Lake Baikal

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Abstract

Bacterial communities of the water and the biofilm formed during five years on an artificial substrate in Lake Baikal were studied by the pyrosequencing of 16S rRNA gene fragments; taxonomic diversity of bacterial communities and differences in their structure were revealed. The biofilm community contained mainly representatives of three phyla: Cyanobacteria, Bacteroidetes, and Proteobacteria; the amounts of other groups were within 1%. Bacterial community of the plankton was more heterogeneous; along with the dominant phyla (Bacteroidetes, Actinobacteria, and Proteobacteria) 15% of the members were of the other phyla. The use of pyrosequencing allowed to reveal 35 bacterial phyla in Lake Baikal, some of which were identified for the first time; moreover, minor groups of microorganisms (including only several sequences), which were not earlier determined by other molecular methods were found.

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Authors and Affiliations

  1. Limnological Institute, Siberian Branch, Russian Academy of Sciences, ul. Ulan-Batorskaya 3, Irkutsk, 664033, Russia

    V. V. Parfenova, A. S. Gladkikh & O. I. Belykh

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  1. V. V. Parfenova
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  2. A. S. Gladkikh
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  3. O. I. Belykh
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Correspondence to A. S. Gladkikh.

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Original Russian Text © V.V. Parfenova, A.S. Gladkikh, O.I. Belykh, 2013, published in Mikrobiologiya, 2013, Vol. 82, No. 1, pp. 94–105.

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Parfenova, V.V., Gladkikh, A.S. & Belykh, O.I. Comparative analysis of biodiversity in the planktonic and biofilm bacterial communities in Lake Baikal. Microbiology 82, 91–101 (2013). https://doi.org/10.1134/S0026261713010128

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