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Chromate-resistant mutants of the yeast Pichia guilliermondii: Selection and properties

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Abstract

Chromate-resistant mutants of the non-conventional yeast Pichia guilliermondii L2 were selected by different methods. The isolated mutants were capable of better growth and higher biomass yield at toxic (1.8–2.4 mM) chromate concentrations than the parent strain. The capacity of the mutants for extracellular chromate reduction and chelation of Cr(III) in the culture liquid was demonstrated. The effectiveness of these processes was shown to correlate with the resistance of P. guilliermondii strains to chromate. Extracellular metabolites of the yeast cells cultivated without chromate were shown to be capable of reducing chromate and forming stable soluble Cr(III)-biocomplexes.

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Authors and Affiliations

  1. Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine

    H. P. Ksheminska, G. Z. Gayda, M. F. Ivash & M. V. Gonchar

Authors
  1. H. P. Ksheminska
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  2. G. Z. Gayda
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  3. M. F. Ivash
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  4. M. V. Gonchar
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Correspondence to G. Z. Gayda.

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Original Russian Text © H.P. Ksheminska, G.Z. Gayda, M.F. Ivash, M.V. Gonchar, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 3, pp. 308–319.

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Ksheminska, H.P., Gayda, G.Z., Ivash, M.F. et al. Chromate-resistant mutants of the yeast Pichia guilliermondii: Selection and properties. Microbiology 80, 314–325 (2011). https://doi.org/10.1134/S0026261711030131

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