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The new alkaliphilic bacteriochlorophyll a-containing bacterium Roseinatronobacter monicus sp. nov. from the hypersaline Soda Mono Lake (California, United States)

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Abstract

Two strains of pink-colored aerobic bacteriochlorophyll a-containing bacteria were isolated from aerobic (strain ROS 10) and anaerobic (strain ROS 35) zones of the water column of Mono Lake (California, United States). Cells of the bacteria were nonmotile oval gram-negative rods multiplying by binary fission by means of a constriction. No intracellular membranes were detected. Polyphosphates and poly-β-hydroxybutyric acid were the storage compounds. Pigments were represented by bacteriochlorophyll a and carotenoids of the spheroidene series. The strains were obligately aerobic, mesophilic (temperature optimum of 25–30°C), alkaliphilic (pH optimum of 8.5–9.5), and moderately halophilic (optimal NaCl concentration of 40 g/l). They were obligately heterotrophic and grew aerobically in the dark and in the light. Respiration was inhibited by light at wavelengths corresponding to the absorption of the cellular pigments. The substrate utilization spectra were strain-specific. In the course of organotrophic growth, the bacteria could oxidize thiosulfate to sulfate; sulfide and polysulfide could also be oxidized. The DNA G+C content was 59.4 mol % in strain ROS 10 and 59 mol % in strain ROS 35. In their phenotypic properties, the new strains were close but not identical to the alkaliphilic bacterium Roseinatronobacter thiooxidans. The distinctions in the nucleotide sequences of the 16S rRNA genes (2%) and low DNA-DNA hybridization level with Rna. thiooxidans (22–25%) allow the new strains to be assigned to a new species of the genus Roseinatronobacter, Roseinatronobacter monicus sp. nov. with the type strain ROS 35T (=UNIQEM U-251T = VKM B-2404T).

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    E. N. Boldareva, I. A. Bryantseva, D. Yu. Sorokin, T. P. Tourova & V. M. Gorlenko

  2. University of Southern California, 233 Samberg Hall, Los Angeles, CA, 90089-0740, USA

    A. Tsapin & K. Nelson

  3. Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    V. A. Boichenko

  4. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia

    I. N. Stadnichuk

Authors
  1. E. N. Boldareva
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  2. I. A. Bryantseva
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  3. A. Tsapin
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  4. K. Nelson
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  5. D. Yu. Sorokin
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  6. T. P. Tourova
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  7. V. A. Boichenko
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  8. I. N. Stadnichuk
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  9. V. M. Gorlenko
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Correspondence to V. M. Gorlenko.

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Original Russian Text © E.N. Boldareva, I.A. Bryantseva, A. Tsapin, K. Nelson, D.Yu. Sorokin, T.P. Tourova, V.A. Boichenko, I.N. Stadnichuk, V.M. Gorlenko, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 1, pp. 95–106.

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Boldareva, E.N., Bryantseva, I.A., Tsapin, A. et al. The new alkaliphilic bacteriochlorophyll a-containing bacterium Roseinatronobacter monicus sp. nov. from the hypersaline Soda Mono Lake (California, United States). Microbiology 76, 82–92 (2007). https://doi.org/10.1134/S0026261707010122

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  • DOI: https://doi.org/10.1134/S0026261707010122

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