Skip to main content
SpringerLink
Log in

Using of silica particles as porogen for preparation of macroporous chitosan macrospheres suitable for enzyme immobilization

  • Published:
Kinetics and Catalysis Aims and scope Submit manuscript

Abstract

Porous chitosan macrospheres were prepared at the first time by using silica particles as porogen, and the optimal weight ratio of silica to chitosan during preparation was determined. Scanning electron microscopy micrographs showed that the support with silica as porogen (support I) had a much more porous surface structure than the support without porogen (support II). Both supports were applied to immobilize β-galactosidase from Aspergillus oryzae. Much higher specific activity and yield of galactose hydrolysis products were observed for the support I. Properties of both immobilized enzyme were determined and compared with the free enzyme, satisfactory results were obtained in thermostability, pH arid operational stability, Michaelis constants K m and in maximum velocity of hydrolysis (V m). Suggested method allow to prepare chitosan macrospheres as immobilized enzyme carrier with moreporous surface structure and more active reaction groups.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nedim, A. and Shangtian, Y., Enzyme Microb. Technol., 2002, vol. 31, p. 371.

    Article  Google Scholar 

  2. De Lathouder, K.M., van Benthem, D.T.J., Wallin, S.A., Mateo, C., Fernandez Lafuente, R., Guisan, J.M., and Kapteljnf Moulijnj, A., J. Mol. Catal., 2008, vol. 50, p. 20.

    Article  Google Scholar 

  3. Abdullah, J., Ahmad, M., Karuppiah, N., Heng, L.Y., and Sidek, H., Sens. Actuators., B, 2006, vol. 114, p. 604.

    Article  Google Scholar 

  4. Gamze, D.A. and Senay, A.C., Food Chem., 2007, vol. 100, p. 964.

    Article  Google Scholar 

  5. Milosavic, N., Prodanovic, R., Jovanovic, S., and Vujcic, Z., Enzyme Microb. Technol., 2007, vol. 40, p. 1422.

    Article  CAS  Google Scholar 

  6. Tarik, D., Sema, T., and Yasemin, K., Food Chem., 2004, vol. 85, p. 461.

    Article  Google Scholar 

  7. Mozaffar, Z., Nakanishi, K., Matsuno, R., and Kamikubo, T., Agric. Biol. Chem., 1984, vol. 48, p. 3053.

    CAS  Google Scholar 

  8. Bisset, F. and Sternberg, D., Appl. Environ. Microbiol., 1978, vol. 35, p. 750.

    Google Scholar 

  9. Muzzarelli, R., Barontini, G., and Rocchetti, R., Biotechnol. Bioeng., 1976, vol. 18, p. 1445.

    Article  CAS  Google Scholar 

  10. Arica, M.Y. and Hasirci, V., J. Chem. Technol. Biotechnol., 1993, vol. 58, p. 287.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Chemistry and Environmental Science, Hebei University, Baoding, China

    Sufang Sun, Yan Zhang, Lingyun Dong & Shigang Shen

Authors
  1. Sufang Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lingyun Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shigang Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sufang Sun.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, S., Zhang, Y., Dong, L. et al. Using of silica particles as porogen for preparation of macroporous chitosan macrospheres suitable for enzyme immobilization. Kinet Catal 51, 771–775 (2010). https://doi.org/10.1134/S0023158410050204

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0023158410050204

Keywords

Search

Navigation