Abstract
It was shown that a 1-h-long exposure of mouse peritoneal neutrophils to a combination of a weak constant magnetic field (42 μT) and low-frequency alternating magnetic fields collinear to the weak constant magnetic field (the sum of the frequencies 1.0, 4.4, and 16.5 Hz; amplitude, 0.86 μT) at physiological temperatures caused an increase in the intracellular production of reactive oxygen species, as measured by the changes in fluorescence of the products of 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123 oxidation. The effect of weak magnetic fields was significantly more pronounced in the presence of low concentrations of respiratory burst activators (N-formyl-Met–Leu–Phe or phorbol 12-meristate-13-acetate).
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Abbreviations
- ROS:
-
reactive oxygen species
- CMF:
-
combined magnetic fields
- fMLP:
-
N-formyl–Met–Leu–Phe
- PMA:
-
phorbol-12-meristate-13-acetate
- H2DCF-DA:
-
2,7-dichlorodihydrofluorescein diacetate
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Original Russian Text © V.V. Novikov, E.V. Yablokova, E.E. Fesenko, 2016, published in Biofizika, 2016, Vol. 61, No. 6, pp. 1159–1163.
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Novikov, V.V., Yablokova, E.V. & Fesenko, E.E. The effect of weak magnetic fields on the production of reactive oxygen species in neutrophils. BIOPHYSICS 61, 959–962 (2016). https://doi.org/10.1134/S0006350916060208
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DOI: https://doi.org/10.1134/S0006350916060208