Abstract
In the present work, it has been shown that isolated mitochondria can undergo transformation to lipofuscin granules without any additional factors (oxygen saturation or prooxidants). The process occurs spontaneously and slowly at low temperature and rapidly upon heating (thermolipofuscin) or UV irradiation (photolipofuscin). The main contribution to the formation of mitochondrial lipofuscin is made by denatured proteins. The formation of thermolipofuscin depends on lipid peroxidation, while the presence of lipids is not required for photolipofuscin formation. It has been shown that the use of a detergent that is able to degrade mitochondria is necessary to measure the lipofuscin content properly.
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Original Russian Text © M.S. Frolova, A.M. Surin, A.V. Braslavski, N.L. Vekshin, 2015, published in Biofizika, 2015, Vol. 60, No. 6, pp. 1125–1131.
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Frolova, M.S., Surin, A.M., Braslavski, A.V. et al. Degradation of mitochondria to lipofuscin upon heating and illumination. BIOPHYSICS 60, 934–939 (2015). https://doi.org/10.1134/S0006350915060081
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DOI: https://doi.org/10.1134/S0006350915060081