Abstract
To elucidate whether a decrease in pHo and pHi has the same effect on presynaptic plasma membrane depolarization, we compared the effects of 1 mM of amiloride which decreased pHi down to 6.65 and incubation medium acidification down to pHo 7.0 and 6.0 on DiSC3(5) dye fluorescence. Measurements were performed with and without the mitochondrial toxins: rotenone (10 μM) + oligomycin (5 μg/mL). In the presence of toxins the change in DiSC3(5) fluorescence was specific for plasma membrane potential. In the second case, DiSC3(5) signal represented the total potential of mitochondria and plasma membrane. It was shown that decreasing of pHo works both in the presence of mitochondrial toxins and also in their absence. Amiloride is able to increase DiSC3(5) fluorescence only in the presence of the mixture of rotenone + oligomycin. Therefore, unlike the mitochondrial potential, the plasma membrane potential is sensitive to a decrease in pHi and pHo.
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Original Russian Text © T.G. Pekun, T.V. Waseem, S.V. Fedorovich, 2014, published in Biofizika, 2014, Vol. 59, No. 1, pp. 100–104.
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Pekun, T.G., Waseem, T.V. & Fedorovich, S.V. Depolarization of plasma membrane of rat brain synaptosomes at extra- and intracellular acidification. BIOPHYSICS 59, 77–80 (2014). https://doi.org/10.1134/S000635091401014X
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DOI: https://doi.org/10.1134/S000635091401014X