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A new enzyme preparation with high penicillopepsin activity based on the producer strain Penicillium canescens

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Abstract

The producer of fungal penicillopepsin, an aspartate protease, has been created by genetic engineering. The biochemical and physicochemical properties of the penicillopepsin enzyme preparation obtained from the culture liquid of the producer were studied. Properties of the new enzyme preparation and the commercially available aspergillopepsin were compared. Their proteolytic activities were found to be 670–680 U/g of the preparation. The soluble protein yield upon the wheat flour hydrolysis with penicillopepsin was 2.7 times higher than with aspergillopepsin. It is probably caused by the presence of the xylanase activity in the penicillopepsin preparation.

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Authors and Affiliations

  1. All-Russia Research Institute of Food Biotechnology, Moscow, 111033, Russia

    I. A. Smirnova, A. S. Sereda, E. V. Kostyleva & N. V. Tsurikova

  2. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia

    E. V. Bushina, A. M. Rozhkova & A. P. Sinitsyn

  3. Lomonosov Moscow State University, Department of Chemistry, Moscow, 119991, Russia

    A. P. Sinitsyn

Authors
  1. I. A. Smirnova
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  2. A. S. Sereda
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  3. E. V. Kostyleva
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  4. N. V. Tsurikova
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  5. E. V. Bushina
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  6. A. M. Rozhkova
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  7. A. P. Sinitsyn
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Correspondence to E. V. Kostyleva.

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Original Russian Text © I.A. Smirnova, A.S. Sereda, E.V. Kostyleva, N.V. Tsurikova, E.V. Bushina, A.M. Rozhkova, A.P. Sinitsyn, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 6, pp. 584–591.

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Smirnova, I.A., Sereda, A.S., Kostyleva, E.V. et al. A new enzyme preparation with high penicillopepsin activity based on the producer strain Penicillium canescens . Appl Biochem Microbiol 51, 660–666 (2015). https://doi.org/10.1134/S0003683815060162

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  • DOI: https://doi.org/10.1134/S0003683815060162

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