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Purification, characteristics and identification of chitinases synthesized by the bacterium Serratia plymuthica MP44 antagonistic against phytopathogenic fungi

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Abstract

Once isolated from the rhizosphere, the chitinolytic bacterium Serratia plymuthica MP44 showed strong antagonistic action against such phytopathogenic fungal species as: Rhizoctonia solani, Cladosporium sp., Alternaria alternata and, to a lesser degree, to the species from the genera Fusarium and Chaetomium. In contrast, no antagonism between the bacteria and Aspergillus niger was observed. S. plymuthica MP44 demonstrated the highest chitinolytic activity on the fifth day of the cultivation on a medium with 1% colloidal chitin. The analysis of zymograms indicated that the studied bacterial strain synthesized 2 chitinolytic enzymes. After purification of culture supernatant of S. plymuthica MP44 with ammonium sulphate precipitation and hydrophobic chromatography on Phenyl-Sepharose CL-4B, 2 active fractions were obtained: fraction I (FI) containing exochitinase activity and fraction II (FII) containing endochitinase activity. The optimum pH and temperature for the purified enzyme were 6.3 and 45°C and 5.4 and 50°C for FI and FII, respectively. High thermal stability was revealed for enzyme from FII after 3 h of incubation at 50°C. The purified chitinases displayed low fungistatic activity when compared to the activity of the bacteria per se.

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References

  1. Sharma, N., Sharma, K.P., Gaur, R.K., and Gupta, V.K., Asian J. Biochem., 2011, vol. 6, no. 1, pp. 29–37.

    Article  CAS  Google Scholar 

  2. Swiontek-Brzezinska, M., Jankiewicz, U., Burkowska, A., and Walczak, M., Curr Microbiol., 2014, vol. 68, no. 1, pp. 71–81.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Vaidya, R.J., Macmil, S.L.A., Vyas, P.R., and Chhatpar, H.S., Lett. Appl. Microbiol., 2003, vol. 36, no. 3, pp. 129–134.

    Article  CAS  PubMed  Google Scholar 

  4. Leclòre, V., Béchet, M., Adam, A., Guez J.S., Wathe let, B., Ongena, M., et al., Appl. Environ. Microbiol., 2005, vol. 71, no. 8, pp. 4577–4584.

    Article  Google Scholar 

  5. Chitin and Chitinases, Jolles, P. and Muzzarelli, R.A.A., Eds., Basel: Birkhäuser Verlag, 1999, pp 171–184.

    Book  Google Scholar 

  6. Hamid, R., Khan, M.A., Ahmad, M., Ahmad, M.M., Abdin, M.Z., Musarrat, J., and Javed, S., J. Pharm. Bioallied Sci., 2013, vol. 5, no. 1, pp. 21–29.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Ubhayasekera, W., Polym. Int., 2011, vol. 60, no. 6, pp. 890–896.

    Article  CAS  Google Scholar 

  8. Jankiewicz, U. and Kołtonowicz, M., Appl. Biochem. Microbiol., 2012, vol. 48, no. 3, pp. 244–249.

    Article  CAS  Google Scholar 

  9. Swiontek Brzezinska M., and Jankiewicz, U., Curr. Microbiol., 2012, vol. 65, no. 6, pp. 666–672.

    Article  Google Scholar 

  10. Sivasakthi, S., Usharani, G., and Saranraj, P., Afr. J. Agric. Res., 2014, vol. 9, no. 16, pp. 1265–1277.

    Google Scholar 

  11. Beneduzi, A., Ambrosini, A., and Passaglia L.M.P., Genet. Mol. Biol., 2012, vol. 35, no. 4, pp. 1044–1051.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Hariprasad, P., Divakara, S.T., and Niranjana, S.R., Crop Prot., 2011, vol. 30, no. 12, pp. 1606–1612.

    Article  Google Scholar 

  13. Jankiewicz, U., Swiontek Brzezinska, M., and Saks, E., J. Biosci Bioeng., 2012, vol. 113, no. 1, pp. 30–35.

    Article  CAS  PubMed  Google Scholar 

  14. Babashpour, S., Aminzadeh, S., Farrokhi, N., Karkhane, A., and Haghbeen, K., Biochem. Genet., 2012, vol. 50, no. 9–10, pp. 722–735.

    Article  CAS  PubMed  Google Scholar 

  15. Someya, N., Ikeda, S., Morohoshi T., Noguchi Tsuji moto M, Yoshida T, Sawada, H., and Tsuchiya, K., Microbes Environ., 2011, vol. 26, no. 1, pp. 7–14.

    Article  PubMed  Google Scholar 

  16. Miller, G.L., Anal. Chem., 1959, vol. 31, no. 3, pp. 426–428.

  17. Lee, H.S., Han, D. S., Choi, S. J., and Choi, S., Appl. Microbiol. Biotechnol., 2000, vol. 54, no. 3, pp. 397–405.

    Article  CAS  PubMed  Google Scholar 

  18. Bradford, M.M., Anal. Biochem., 1976, vol. 72, no. 1–2, pp. 248–254.

    Article  CAS  PubMed  Google Scholar 

  19. Laemmli, U.K., Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  20. Trudel, J. and Asselin, A., Anal. Biochem., 1989, vol. 178, no. 2, pp. 362–366.

    Article  CAS  PubMed  Google Scholar 

  21. Stachowiak, B., Trojanowska, K., and Gulewicz, K., Prog. Plant Prot., 2007, vol. 47, no. 2, pp. 338–342 (in Polish).

    Google Scholar 

  22. Spadaro, D. and Gullino, M.L., Crop Prot., 2005, vol. 24, no. 7, pp. 601–613.

    Article  Google Scholar 

  23. Kordowska-Wiater, M., Adv. Microbiol., 2011, vol. 50, no. 2, pp 107–119 (in Polish).

    Google Scholar 

  24. Mane, U.V. and Deshmukh, A.M., Afr. J. Biotechnol., 2009, vol. 8, no. 23, pp. 6617–6620.

    CAS  Google Scholar 

  25. Hosny, M.S., El-Shayeb, N.A., Abood, A., and Abdel-Fattah, A.M., Australian J. Basic Appl. Sci., 2010, vol. 4, no. 4, pp. 615–623.

    CAS  Google Scholar 

  26. Wang, S.L., Hsiao, W.J., and Chang, W.T., J. Agric Food Chem., 2002, vol. 50, no. 8, pp. 2249–2255.

    Article  CAS  PubMed  Google Scholar 

  27. Swiontek Brzezinska, M., Jankiewicz, U., and Walczak, M., Internat. Biodet. Biodeg., 2013, vol. 84, pp. 104–110.

    Article  Google Scholar 

  28. Frankowski, J., Lorito, M., Scala, F., Schmid, R., Berg, G., and Bahl, H., Arch. Microbiol., 2001, vol. 176, no. 6, pp. 421–426.

    Article  CAS  PubMed  Google Scholar 

  29. Kim, H.S., Timmis, K.N., and Golyshin, P.N., Appl. Microbiol. Biotechnol., 2007, vol. 75, no. 6, pp. 1275–1283.

    Article  CAS  PubMed  Google Scholar 

  30. Rout, S.K., Jayachandran, S., and Prinyawiwatkul, W., Appl. Environ. Microbiol., 2000, vol. 6, no. 4, pp. 112–121.

    Google Scholar 

  31. Alstrom, S. and Gerhardson, B., Plant Soil, 1987, vol. 103, no. 2, pp. 185–189.

    Article  Google Scholar 

  32. Kamensky, M., Ovadis, M., Chet, I., and Chernin, L., Soil Biol. Biochem., 2003, vol. 35, no. 2, pp. 323–331.

    Article  CAS  Google Scholar 

  33. De Vleesschauwer D., and Höfte, M., CAB Reviews, 2007, vol. 2, no. 046.

    Google Scholar 

  34. Someya, N., Nakajima, M., Hirayae, K., Hibi, T., and Akutsu, K., J. Gen. Plant Pathol., 2001, vol. 67, no. 4, pp. 312–317.

    Article  CAS  Google Scholar 

  35. Roberts, W.K. and Selitrennikoff, C. P., J. Gen. Microbiol., 1988, vol. 134, no. 1, pp.169–176.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Warsaw University of Life Sciences, SGGW, 02-776, Warsaw, Poland

    U. Jankiewicz

  2. Department of Environmental Microbiology and Biotechnology, Institute of Ecology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland

    M. Swiontek Brzezinska

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  1. U. Jankiewicz
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Jankiewicz, U., Swiontek Brzezinska, M. Purification, characteristics and identification of chitinases synthesized by the bacterium Serratia plymuthica MP44 antagonistic against phytopathogenic fungi. Appl Biochem Microbiol 51, 560–565 (2015). https://doi.org/10.1134/S0003683815050105

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  • DOI: https://doi.org/10.1134/S0003683815050105

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