Moleculo Long-Read Sequencing Facilitates Assembly and Genomic Binning from Complex Soil Metagenomes

Soil microorganisms carry out key processes for life on our planet, including cycling of carbon and other nutrients and supporting growth of plants. However, there is poor molecular-level understanding of their functional roles in ecosystem stability and responses to environmental perturbations. This knowledge gap is largely due to the difficulty in culturing the majority of soil microbes. Thus, use of culture-independent approaches, such as metagenomics, promises the direct assessment of the functional potential of soil microbiomes. Soil is, however, a challenge for metagenomic assembly due to its high microbial diversity and variable evenness, resulting in low coverage and uneven sampling of microbial genomes. Despite increasingly large soil metagenome data volumes (>200 Gbp), the majority of the data do not assemble. Here, we used the cutting-edge approach of synthetic long-read sequencing technology (Moleculo) to assemble soil metagenome sequence data into long contigs and used the assemblies for binning of genomes.


IMPORTANCE
Soil microorganisms carry out key processes for life on our planet, including cycling of carbon and other nutrients and supporting growth of plants. However, there is poor molecular-level understanding of their functional roles in ecosystem stability and responses to environmental perturbations. This knowledge gap is largely due to the difficulty in culturing the majority of soil microbes. Thus, use of culture-independent approaches, such as metagenomics, promises the direct assessment of the functional potential of soil microbiomes. Soil is, however, a challenge for metagenomic assembly due to its high microbial diversity and variable evenness, resulting in low coverage and uneven sampling of microbial genomes. Despite increasingly large soil metagenome data volumes (Ͼ200 Gbp), the majority of the data do not assemble. Here, we used the cutting-edge approach of synthetic long-read sequencing technology (Moleculo) to assemble soil metagenome sequence data into long contigs and used the assemblies for binning of genomes.
KEYWORDS: de novo assembly, Moleculo, metagenomic assembly, metagenomic binning, soil metagenomics S oil metagenomics has been termed the "grand challenge," due to the complexity and diversity of the microbial communities in these ecosystems (1)(2)(3)(4). Soils represent one of the most diverse ecosystems on the planet (1), with estimates of billions of microbial cells and~100,000 unique bacterial and archaeal species in 1 g of soil (2). Analysis of existing soil metagenomic data has also resulted in high microbial genetic diversity estimates, equivalent to~10 12 genes per gram of soil (2). This high diversity represents an enormous challenge to metagenomics, due to low coverage obtained for individual populations, uneven sampling of microbes, and the large amount of sequence data acquired, often in short DNA fragments (~100 to 150 bp) (3,4). It has been estimated that tera-base pairs (Tbp) of sequence data would be required to adequately sample a single gram of soil if using current metagenomic sequencing and assembly platforms (3)(4)(5)(6).
To address this challenge of soil metagenome assembly and annotation, the Department of Energy's Joint Genome Institute (JGI) initiated the Great Prairie Soil Metagenome Grand Challenge Initiative (1), which included native prairie and adjacent long-term agricultural soil locations in Wisconsin, Iowa, and Kansas. As a result of the JGI initiative, the two largest soil metagenomes published to date are from Iowa native prairie soil, containing 3.3 billion reads, or 257 Gbp of raw data, and from the adjacent cultivated soil ("continuous corn"), containing 1.8 billion reads or 141 Gbp (4). However, only nine sequences of Ͼ10 kbp could be assembled from the continuous corn, and none was Ͼ10 kbp from the native Iowa prairie soil assembly. Approximately 80% of the sequencing data could not be assembled, and less than 11% of the reads mapped to either the assembly from the continuous corn or from the native prairie soil (4). Thus, novel genomes, genes, and functional guilds have yet to be discovered among the vast microbial diversity present in soil ecosystems.
Of the JGI soil metagenomes resulting from the Grand Challenge project, the largest amount of sequence data was obtained from the Kansas native prairie (Ͼ1.3 Tbp). Therefore, we focused on Kansas native prairie soil as the target metagenome with the aim of further advancing sequencing and assembly approaches to attempt to tackle some of the challenges inherent to soil metagenomics.
A promising approach for improving metagenome analysis is de novo assembly. A de novo assembly approach provides error correction of short reads, connects fragmented protein-coding open reading frames (ORFs), reduces data volume, and enables higher-quality functional gene annotation and better phylogenetic taxonomic assignments than do gene-centric-based approaches (4). By comparison, unassembled short reads of Ͻ150 bp offer few solutions for annotation and phylogenetic approaches, as they are often too short for reliable analysis (7), and short read length alone can cause artifacts (8). De novo assembly is therefore often the first step in metagenomic analysis after data trimming and cleaning (9). However, this approach to metagenomics currently has three major caveats: (i) it does not capture long-range sequence contiguity, as community DNA is fragmented commonly to Ͻ1-kbp lengths (10); (ii) it masks individual strain-level genotypes due to pooling of community DNA (10); (iii) current assembly algorithms do not scale computationally for complex soil ecosystems due to the high diversity and large data volume (4).
In addition, due to the assembly issues associated with soil, it has been difficult to reconstruct genomes from complex soil metagenomes. However, genomes have been successfully binned from lower-complexity metagenomes, including acid mine drainage (11), deep aquifers (12), mat microcosms (13), wastewater bioreactors (14,15), permafrost soil (16,17), and the human gut (18). Recently, the complexity of soil was reduced after incubating samples under harsh treatment conditions (e.g., in the presence of heavy metals, salts, and ethanol), and this improved metagenome assembly and allowed for direct binning of genomes (19).
A promising alternative approach to address the challenges in soil metagenome assembly is to utilize Moleculo hybrid synthetic long reads generated via the high-throughput sequencing Illumina platform, also known as Illumina TruSeq long-read hybrid subassembly (20). The Moleculo technology can provide Ͼ8-kbp subassembled contig lengths with 99.9% accuracy (20)(21)(22) and is summarized in Fig. S1A in the supplemental material. Recently, metagenomic studies conducted on sediments (23) and the human gut microbiome (24) have demonstrated that Moleculo alone, or hybrid assembly with short reads and Moleculo, can generate long contigs of Ͼ100 kbp.
The objectives of our study were (i) to evaluate whether the Moleculo technology alone would improve soil metagenome assembly and allow for genome binning from a complex soil ecosystem; (ii) to compare and contrast the Moleculo assemblies to metagenome assemblies from three different Illumina read-length formats, including short reads with 100-bp paired ends (here called short reads [SR]), rapid-mode reads of 250-bp paired ends (here called rapid mode reads [RMR]), and reads from Moleculo only, which were sequenced using rapid-mode reads of 250-bp paired ends and then subassembled; (iii) to reconstruct functionally active pathways by mapping metatranscriptome reads to the Moleculo assembly; (iv) to reconstruct functionally active genome bins by mapping metatranscriptome reads to the genome bins.

RESULTS
Moleculo subassembly provides long and accurate reads and improves assembly of complex metagenomes. We analyzed a combination of existing metagenome sequence data from native prairie soil that was previously collected at the Konza Prairie Station in Kansas and sequenced at the JGI. We also performed additional sequencing of fresh soils from three additional locations of the same Kansas native prairie (sites A, B, and C). We obtained approximately 300 million raw reads (69.7 Gbp of raw data) using the Moleculo technology and the majority were long, with 15,400 contigs of Ͼ9 kbp, 5,600 contigs of Ͼ10 kbp, and a total assembly of 775 Mbp on contigs of Ͼ1.5 kbp (Table 1; see also Fig. S1B in the supplemental material). The quality of the data obtained using Moleculo was on average ϾQ 30 (a Q 30 score represents a probability of an incorrect base call of 1 in 1,000, or 99.9% accuracy), suggesting Sanger-like accuracy (see Fig. S1C).
We also compared the Moleculo data to metagenome assemblies obtained using SR 100-bp paired-end data, previously generated at the JGI, and Illumina HiSeq RMR 250-bp paired-end reads ( Table 1). The MegaHit assembler was used to assemble the data from the SR and RMR sequencing approaches, resulting in Ͼ30% overall read mappability to contigs of Ͼ1 kbp in length (Table 1) (25). The SR assembly resulted in 827 contigs of Ͼ9 kbp, 604 contigs of Ͼ10 kbp, and a total assembly of 520 Mbp on contigs of Ͼ1 kbp. The RMR assembly resulted in 936 contigs of Ͼ9 kbp, 584 contigs of Ͼ10 kbp, and a total assembly of 1.2 Gbp on contigs of Ͼ1 kbp. Moleculo had 98.6% overall read mappability or alignment to contigs of Ͼ1.5 kbp in length (as none was below 1.5 kbp), suggesting most of the read data were represented in contigs (Table 1). Among Moleculo-only contigs, 67% were Ͼ5 kbp; by comparison, Ͻ1.5% of either the RMR or SR assemblies were represented in contigs of Ͼ5 kbp (Table 1). In addition, Moleculo alone provided~10 times more long contigs (Ͼ10 kbp) than either SR or RMR assemblies (Table 1). a "Moleculo only" indicates the subassembled contigs from the TruSeq synthetic long-read DNA library prep (Illumina). Values in the column for data size indicate the total raw data obtained with each format. Read lengths for short reads are in the HiSeq 100-bp paired-end format, whereas rapid mode reads and Moleculo-only reads were sequenced in the HiSeq rapid mode in the 250-bp paired-end format. Size represents the total assembly size, and "Reads mapped" data represent the total unassembled reads mapped to contigs of Ͼ1 kbp in length overall, determined using Bowtie2. The number of contigs of Ͼ5 kb includes all contigs for the given length cutoff.
It has been challenging to reconstruct full-length 16S rRNA gene sequences from complex metagenomes, with few successful examples to date (29). Here, we compared the RMR sequence assembly to the Moleculo subassembly for achievability of longer 16S rRNA gene sequences. The Moleculo assembly yielded double the number of positive 16S rRNA gene identifications (197) (Fig. 1B) than the RMR assembly, with an average sequence length of~1,268 bp; 127 were full-length sequences of~1,400 bp or greater (Fig. 1B). In contrast, RMR assembly captured only 89 assembled 16S rRNA sequences, with an average sequence length of~597 bp, and only five sequences were obtained that were Ͼ1,400 bp (Fig. 1C).
Moleculo subassembly captures of enzyme functional potential in a complex soil community. The Moleculo metagenome annotation was completed by using Metapathways2 to generate a MetaCyc pathway genome database (ePDGB) with pathway tools for Kansas native prairie soil (30). Of the 354,863 genes in the Moleculo metagenome annotation, 112,704 (32%) were assigned an Enzyme Commission (EC) number, and of the 5,684 official EC enzymes, 1,461 (26%) were assigned to at least 1 gene in the Moleculo metagenome annotation. Of the 2,260 MetaCyc pathways identified from 2,600 organisms, 352 (16%) were predicted for the Moleculo metabolic annotation.
We annotated the functional potentials for enzymes in each of the four assemblies (SR, RMR, Moleculo only, and hybrid) by using the Kyoto Encyclopedia of Genes and Genomes (KEGG; http://www.genome.jp/kegg/) and Clusters of Orthologous Groups (COG; http://www.ncbi.nlm.nih.gov/COG/) ontology assignments, and we obtained taxonomic assignments using RefSeq (http://www.ncbi.nlm.nih.gov/refseq/). We found that both the taxonomic and functional potentials among various assemblies were quite similar ( Fig. 2A to C), although there were slightly more Actinobacteria in the RMR and SR assemblies than in the Moleculo-only and hybrid assemblies ( Fig. 2A).
Metagenome data only reveal information about the metabolic potential of a system, but not all genes are expressed at any given time. Therefore, we sequenced RNA (i.e., metatranscriptomes) from Kansas soils A, B, and C by using the RMR format and found that 26 to 27% of the metatranscriptome sequencing (whole-community RNA-Seq) reads from each of the soil replicates could be mapped to the Moleculo assembly, suggesting that a quarter of the genes were expressed at the time of sampling. Of the 1,461 EC numbers that were annotated in the Moleculo-only assembly, 1,391 (95%) were expressed in at least one soil sample and 1,252 (86%) were expressed in all three samples (Fig. 3D). Less than 2% of the expressed proteins with EC assignments were unique to any one of the three samples (Fig. 3D).
Moleculo subassembly provides genomic resolution via binning. We compared binning quality by using the resulting assemblies from the three assembly methods: SR, RMR, and Moleculo only. We obtained 129 genomic bins from the Moleculo-only data, based on matching 107 hidden Markov model (HMM) marker genes that cover~95% of all bacteria, with an average marker count of 1.39 for each marker per bin using the MaxBin binning system (see Fig. S2 in the supplemental material) (32)(33)(34). The average completeness (the percentage of 107 HMM marker genes covered) was~2% higher with Moleculo than with SR assemblies and~16% higher than with RMR assemblies ( Table 2). The RMR assembly resulted in more genome bins (225 bins) than either the Moleculo or SR assemblies, with 129 and 110 bins, respectively, for the 107 HMM genes ( Table 2). Of the three assemblies, Moleculo had generally higher average genome completeness on fewer longer contigs.
The genome bins contained a diversity of metabolic potential for metabolism of complex macromolecules and elemental cycling. Functional annotations of the bins suggested that all of them had diverse glucoside hydrolyases (GHs) and glycosyltransferases (GTs), predicted by CAZy notation (Fig. 4B). All bins also had high abundances  of predicted protein-coding ORFs for cycling of carbon through central carbohydrate metabolism and cellular respiration (Fig. 4B). The Acidobacteria and Bacteroidetes bins had higher abundances of GHs and genes related to monosaccharide utilization (Fig. 4B). The Bacteroidetes were also predicted to have more iron-related pathways than other bins (Fig. 4B), mainly from siderophores and transporters. We obtained the first complete genome from a native complex soil by using metagenome binning. The genome bin is taxonomically related to the genus Pseudomonas (see Fig. S3 in the supplemental material), specifically, "Candidatus Pseudomonas sp. strain JKJ-1." Based on its full-length 16S rRNA gene sequence (1,531 bp), its closest relatives in the NCBI database include Pseudomonas fluorescens strain FW300-N2E3 (accession number CP012830; 89% identity). "Candidatus Pseudomonas sp. strain JKJ-1" is a complete genome of 6,408,606 bp, with 6,128 protein-coding open reading frames (see Fig. S4 in the supplemental material), 59 tRNAs, and 1 rRNA operon, based on RAST annotation (35).

Genome bins provided by Moleculo subassembly captured metabolically active members of the community.
Metatranscriptome mRNA reads were mapped to the genome bins as a means of determining which of the represented microbes were transcriptionally active (Fig. 5). Some of the genome bins had many transcript reads that could be mapped, whereas others contained very few. Genome bins from soil sample B were more transcriptionally active than those from samples A or C (Fig. 5). Bins 46 and 73, both Acidobacteria bins, were highly transcriptionally active across the samples (Fig. 5A). Although Verrucomicrobia were highly abundant based on the 16S rRNA analysis (Fig. 1) and bin abundance (Fig. 4A), few transcripts mapped back to the Verrucomicrobia bins (Fig. 5B), suggesting that the represented microbes were dormant or otherwise in a state of low transcriptional activity at the time of sampling.

DISCUSSION
Here, we successfully applied the Moleculo technology to address one of the biggest challenges facing metagenomics: the assembly of highly complex soil microbial communities (36). Using Moleculo sequencing alone, we obtained~70 Gbp of data with more than 5,000 reads longer than 10 kbp, and Ͼ95% of the reads could be mapped back to subassembled data (~1.5 kbp in length). By comparison, using SR sequencing, assembly of the Iowa prairie soil, with Ͼ400 Gbp of data, provided only 9 contigs that were Ͼ10 kbp, with only 10.72% of the reads mapping to contigs (4). Here, we found that via hybrid assembly of all of the existing sequence data from Moleculo, SR, and RMR technologies (~267 Gbp), more than 10,000 contigs were obtained that were Ͼ10 kbp in length. Thus, this represents the highest-quality and most extensive metagenome assembly of a soil microbial community to date.
Moleculo library preparation is currently more expensive (~10ϫ more) than use of standard shotgun libraries. However, a single library sequenced using the Moleculo technology represents a reduction in the amount of sequencing throughput required to obtain longer contigs, with~9ϫ more Ͼ10-kbp contigs than with SR or RMR sequencing technologies. The hybrid assembly nearly doubles the number of long contigs that are Ͼ10 kbp compared to Moleculo alone and increases by~17ϫ the number compared to SR or RMR alone. Moleculo also provides a cost savings by reducing downstream assembly costs for complex metagenomes. Another benefit of Moleculo is lower DNA input requirements (~500 ng) and DNA molecule lengths of~10 kbp, which is the average length obtained using standard protocols by the Earth Microbiome Project (EMP) (http://www.earthmicrobiome.org/). Currently, we recommend that for large studies with many samples, it would be appropriate to use standard metagenomic sequencing pipelines and then to add a smaller number of Moleculo libraries to facilitate assembly only for highly complex ecosystems in order to save costs. However, if cost is not an issue, incorporation of at least some Moleculo data will help improve assembly and facilitate binning for basically any environmental sample metagenome.
Chimeras and misassembled contigs are possible from metagenomic and/or metatranscriptomic assemblies. Although the Moleculo technology results in "excellent metagenomic assemblies" (37), the choice of assembler used for subassembling Moleculo reads matters more than the diversity or complexity of the sample when it comes to quality and potential for misassemblies (37,38). Although there are few publications to date describing use of the Moleculo technology, those that have been reported have found no evidence of chimeras (23) and few misassemblies in Moleculo contigs (23,24). Sharon et al. (23) found no evidence of chimeras when mapping Moleculo subassembled contigs to a short-read-generated genomic bin and only two sequences were misassembled. Kuleshov et al. (24) also found low misassemblies from Moleculo technology, as "accuracy was high, with less than 0.5% of reads misassembled." Kuleshov et al. (24) suggested that combining two complementary sequencing technologies, such as SR and/or RMR with Moleculo data, could enhance the overall quality of the assemblies, and this is supported by our findings. This is the first successful demonstration of binning of hundreds of genomes from a highly complex soil type. We predict that even more complete genomes would have been obtained from lower-complexity samples or by adding additional Moleculo data for high-complexity samples. Assembly of the highly accurate long contigs from Moleculo resulted in 129 genome bins with a low marker gene average for each marker per bin. Many diverse phyla were represented in the genome bins, including a complete genome for a novel pseudomonad ("Candidatus Pseudomonas sp. strain JKJ-1"). The majority of bins were related to common soil microbes, including nitrogenfixing Proteobacteria (i.e., Rhizobium), but many of the genomes represent phyla with few cultured representatives, including Verrucomicrobia and Acidobacteria. Of the Verrucomicrobia bins, the closest reference genome was from the Spartobacteria class, which represents a globally dominant soil group that is poorly understood (39). The Acidobacteria bins were classified to the closest known representative reference genome as a candidate uncultured Acidobacteriaceae bacterium KBS83, reflecting the paucity and likely microdiversity of genomes from this phylum. It has been suggested that Acidobacteria on average constitute 20% of all the bacteria in soil (40); however, with few cultured representatives, metagenome binning or single-cell genomics may be the best route to obtain genomes from within this phyla.
The majority of the metabolic predictions in the genome bins suggest that the carbon utilization pathways are very similar across the genomes. This is likely due to the diverse carbon profiles found in prairie soils that have originated from plant root exudates, decaying plant matter, or other organic material (41). These plant root exudates or other rhizosphere inputs likely shape the microbial composition and functional pathways carried out by the native prairie soil community (41).
We mapped metatranscriptome data to predict which of the genome bins represented active members of the soil ecosystem. Genome bins from Firmicutes, Proteobacteria, and Actinobacteria were the most transcriptionally active based on the high number of RNA-Seq reads that mapped to them. Interestingly, although Acidobacteria and Verrucomicrobia both had high relative abundances (on the DNA level) in the soils, the Verrucomicrobia bins showed little transcriptional activity (negligible mapping of RNA-Seq reads) whereas the Acidobacteria bins showed high transcriptional activity. Recent data, using propidium monoazide (PMA)-treated soil, suggest that on average 40% of the prokaryotic and fungal DNA in soil is relic DNA representing microbes that had previously lived in the soil but are not metabolically active (42). Upon PMA treatment and once relic DNA was removed, the number of Verrucomicrobia operational taxonomic units (OTUs) decreased (42). These data support our findings and suggest that the Verrucomicrobia represented by the genomic bins were dormant or relic DNA that had low activity at the time of sampling, in contrast to the Acidobacteria, which were highly transcriptionally active at the time of sampling.
Recovery of full-length 16S rRNA gene sequences from metagenomes has been regarded as an "outstanding challenge" (43), due to short error-prone reads and repetitive regions of rRNA that are troublesome for de novo assemblers. Here, by use of long-read Moleculo sequencing technology, we were able to reconstruct more than 100 full-length (~1.4-kbp) 16S rRNA gene sequences from complex soil metagenomes for the first time. The previous best example was from an oil reservoir metagenome, an environment with lower microbial diversity than soil, where 57 unique 16S rRNA gene sequences of approximately 960 bp in length were recovered (44). We noticed a higher abundance of Planctomyces in our 16S PCR amplicon data than in our metagenome assemblies. Our hypothesis is that because Planctomycetes and Verrucomicrobia are in the same PVC superphylum (Planctomycetes-Verrucomicrobia-Chlamydiae), the primers could amplify members that are hard to resolve in short paired-end reads (~250 bp) but that are possible to resolve in the longer sequences obtained in the metagenome assemblies. In addition, the candidate SPAM phylum evaded detection in our 16S amplicon analysis due to poor forward primer matches in those sequences, but it was detectable in the Moleculo assemblies. Moleculo is thus able to recover many 16S rRNA genes that are nearly or fully complete, directly from complex microbial samples such as soil, with less bias than amplicon-based sequencing.
Finally, Moleculo subassembly and/or hybrid assembly should provide a better scaffold for other database-dependent multi-omics data sets (e.g., metaproteomics and metatranscriptomics). The high-resolution database curated from Moleculo reads allows for near-genome-level annotation from a highly complex microbial community located in the Kansas native prairie and binning of hundreds of microbial genomes from a native prairie soil.
Given these results from a highly complex soil community, we anticipate and encourage the application of Moleculo technology as a broadly applicable approach for resolving highly complex microbial ecosystems across a range of sample types, to the genome scale.

MATERIALS AND METHODS
Site description and sample collection. Soil samples were collected from the upper 20 cm of soil at three watershed locations at the Konza Prairie Biological Station (KPBS), a long-term ecological research (LTER) site located in eastern Kansas, USA. Each soil collected represented a field replicate, here referred to as samples A, B, and C, and was shipped on ice to PNNL, where samples were immediately sieved (2 mm 2 ) to remove root fragments, aliquoted into 50-g portions in Falcon tubes, immediately frozen under liquid nitrogen, and stored at Ϫ80°C until use.
Nucleic acid extraction (DNA and RNA). DNA and RNA were extracted using the PowerSoil DNA and RNA isolation kits, respectively, from MoBio (Carlsbad, CA, USA) in accordance with the manufacturer's instructions. RNA was DNase treated using Turbo DNase (Life Technologies, Grand Island, NY, USA), and then samples were purified by phenol-chloroform extraction followed by ethanol precipitation. Samples were quantified using the Qubit Fuorometer 2.0 (Invitrogen, Carlsbad, CA, USA), quality checked using a NanoDrop apparatus (Thermo Fisher, Waltham, MA, USA) and Bioanalyzer Pico RNA and HighSens DNA chips (Agilent, Santa Clara, CA, USA).
16S rRNA I-Tag sequencing. Amplification of the V4 region of the 16S rRNA gene was performed in accordance with the Earth Microbiome Project recommendations (46)(47)(48)(49), using primers 515f/926r, and sequencing was performed on an MiSeq sequencer (Illumina, San Diego, CA, USA). The QIIME pipeline (version 1.9.1) was used to demultiplex and quality filter data after sequencing. The VSEARCH (version 1.9.10) implementation of UCHIME de novo was used for chimera removal, followed by de novo OTU clustering (45)(46)(47)(48). Of the total unique amplicons,~22% were flagged as chimeric and were removed from any further analysis. Taxonomy annotation and tree building were completed with QIIME defaults, which make use of the Greengenes database (http://greengenes.lbl.gov), UCLUST (49), and FastTree2 (50).
Metagenome and metatranscriptome library preparation. Replicate metagenomes and metatranscriptomes were constructed in triplicate for Kansas soils A, B, and C. DNase-treated and purified RNA was reverse transcribed using the SuperScript VILO cDNA synthesis kit (Life Technologies, Grand Island, NY, USA), according to the manufacturer's protocol, using a 1:1 mixture of 1 M random hexamers/ decamers. Second-strand synthesis was performed using the NEBNext mRNA second-strand synthesis module (NEB, Ipswich, MA, USA) with addition of 10 g T4 gene 32 (NEB, Ipswich, MA, USA), according to the manufacturer's protocol. Double-stranded cDNA was purified using AmpureXP beads (Beckman Coulter, Danvers, MA, USA) and then quantified and quality checked with Bioanalyzer high-sensitivity DNA chips (Agilent, Santa Clara, CA, USA). rRNA depletion was not performed, and rRNA sequences were instead removed computationally using SortMeRNA (51).
Illumina library construction was completed as previously described (53,54). Libraries were quantified by quantitative PCR on an StepOne Plus system (Applied Biosystems, Foster City, CA) using the KAPA library quantification kit (KAPA Biosystems, Wilmington, MA, USA) according to the manufacturer's instructions. RMR technology was used to sequence metatranscriptomes and metagenomes from three separate locations in the Kansas native prairie (soils A, B, and C) using MiSeq (300-bp paired ends) and HiSeq (250-bp paired ends) technologies (Illumina, San Diego, CA, USA) for RNA and DNA, respectively.
Metagenome and metatranscriptome analysis. The paired-end shotgun reads for both metagenomics and metatranscriptomics analyses were overlapped and quality filtered as outlined in references 52 and 53. The resulting decontaminated (i.e., X174) reads were then trimmed for quality at ϽQ 25 by using the trimmomatic program (54). Short reads generated previously at JGI were 100-bp, paired-end reads and were trimmed to~80 bp. Rapid mode reads from replicate metagenomes and metatranscriptomes from Kansas soils A, B, and C were trimmed to~225 bp. Both SR and RMR, were assembled using MEGAHIT (25) with mixed k-mers (21 to 121 k).
The contigs were annotated using MetaPathways2 as previously described (52,53). Metatranscriptomes and metagenomes were mapped to assemblies and genome bins using Bowtie2 (31). The gene counts per enzyme (EC number) and transcript reads per EC number were mapped onto the Moleculo annotation metabolic pathways in the BioCyc framework (55). Full-length 16S rRNA gene extraction from Moleculo and RMR contig data was completed within Metapathways2 by using LAST (E value, Ͻ1E Ϫ7 ) annotations with the Greengenes database. 16S rRNA genes from Moleculo-only contigs and RMR contigs were chimera checked with the VSEARCH implementation of UCHIME de novo, followed by UCHIME ref against the Greengenes database. Four sequences were initially flagged as potential chimeras, but upon manual investigation we found that low-quality bases in the reference database were the source of mismatches that spuriously inflated UCHIME scores and flagged these reads as chimeric. The data analysis workflow is outlined in Fig. S5 in the supplemental material.
Moleculo long-hybrid-read subassembly library preparation and analysis. Our Moleculo library preparation and long-hybrid-read subassembly using Illumina standard protocols are outlined in Fig. S1 and S5 in the supplemental material and also in the original description of Moleculo (20). DNA from the nine replicate extractions from the A, B, and C soil samples were pooled into a single long-hybrid library prep, following the manufacturer's protocol (Illumina). The resulting data were assembled on BaseSpace by using the Illumina TruSeq long-read assembly app v1.0.
Combined assembly merge, genome binning, and genome bin annotation. After SR (~80-bp) and RMR (~225-bp) reads were assembled with MegaHit into contigs, they were pooled with the Moleculo subassembled reads and further assembled using CAP3 (26). Pooled contigs from the various formats were selected for Ͼ4-kbp contigs and then assembled with CAP3 and merged using minimus2 (21-24, 27, 28). Contigs of Ͼ1 kbp were binned using MaxBin (32)(33)(34) with 107 HMM marker genes and then parsed using the R program. Genome bins were then quality checked with mapping reads using Bowtie2. Annotation and metabolic reconstruction of contigs and genomic bins were completed using Metapathways2 (30), the FOAM database (56), and hmmer3.1 (57) to obtain a list of KEGG ontology numbers. The taxonomic and phylogenetic identifications of the genome bins were obtained using the JspeciesWS Tetra Correlation Search (TCS) (58), and then cross-referenced annotations were obtained by using Metapathways2 and FOAM.
MaxBin binning of short reads from JGI resulted in a novel~99% complete genome based on 107 HMM markers; this genome was named "Candidatus Pseudomonas sp. strain JKJ-1." Short and Moleculo subassembled contigs were mapped using Bowtie2 and then used for error correction. Genome circulation and closure of "Candidatus Pseudomonas sp. strain JKJ-1" was completed by using minimus2 and then finished with the EMBOSS union script followed by manual checking (59). The genome was plotted using the CGviewer server with default parameters (60). The whole-genome-based alignment tree was constructed in the Department of Energy Systems Biology Knowledge Base (KBase; http://kbase.us), using COG domains and FastTree2 (50). Annotation was conducted on the RAST Web server with RAST gene calling based on FIGfam, release 70, and full metabolic reconstruction was performed using ModelSEED (61).
Nucleotide sequence accession numbers. The RMR and Moleculo-only 16S rRNA sequences have been deposited in GenBank under accession numbers KX239231 to KX239311 and KX239037 to KX239230. All other data have been deposited in the SRA under our manuscript title, with accession numbers listed in Table S2 of the supplemental material.
collecting the soil samples used in our study. We also thank Susannah Tringe, Alex Copeland, and their teams at the DOE JGI for providing the short-read sequence data through the Great Prairie Grand Challenge Sequencing Initiative at JGI and for their discussions regarding metagenome assembly. We thank William Nelson for comments on the draft manuscript. We further thank Yiyong (Ben) Liu at Washington State University Health Sciences, Spokane, WA, for performing the HiSeq rapid-mode sequencing and the Moleculo library preparation.

FUNDING INFORMATION
The research in this study, including the efforts of Janet K. Jansson