SARS-CoV-2 B.1.1.7 Infection of Syrian Hamster Does Not Cause More Severe Disease, and Naturally Acquired Immunity Confers Protection

ABSTRACT Epidemiological studies have revealed the emergence of multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOC), including the lineage B.1.1.7 that is rapidly replacing old variants. The B.1.1.7 variant has been linked to increased morbidity rates, transmissibility, and potentially mortality. To assess viral fitness in vivo and to address whether the B.1.1.7 variant is capable of immune escape, we conducted infection and reinfection studies in naive and convalescent Syrian hamsters (>10 months old). Nasal wash samples from hamsters infected by a B.1.1.7 variant exhibited slightly higher viral RNA levels but lower infectious titers than those from B.1 (G614) variant-infected hamsters, and the two variants induced comparable lung pathologies in hamsters. Despite a sporadic and transient low-level infection in the nasal cavity, convalescent hamsters that had recovered from a previous USA-WA1 isolate (D614) infection displayed no observable clinical signs or lung pathology following B.1.1.7 rechallenge. Altogether, our study did not find that the B.1.1.7 variant significantly differs from the B.1 variant in pathogenicity in Syrian hamsters and that a heterologous natural infection-induced immunity confers protection against a secondary challenge by the B1.1.7 variant. IMPORTANCE The rapid emergence of several variants of concern of SARS-CoV-2 calls for evaluations of viral fitness and pathogenicity in animal models in order to understand the mechanism of enhanced transmission and the possible increases in morbidity and mortality rates. Here, we demonstrated that immunity naturally acquired through a prior infection with the first-wave variant does confer nearly complete protection against the B.1.1.7 variant in Syrian hamsters upon reexposure. Strikingly, although the B.1.1.7 variant appears to replicate to a higher level in the nose than the ancestral B.1 variant, it does not induce more severe lung pathology in hamsters.

been found to increase the binding affinity of spike protein to the human ACE2 receptor, resulting in an elevated transmissibility and monoclonal antibody resistance (8)(9)(10).
Here, we set out to address whether a prior exposure to an ancestral SARS-CoV-2 (D614) isolate would offer protection against reinfection by a B.1.1.7 variant. In addition, we investigated whether the B.1.1.7 variant displays fitness advantage over the G614 variant in the Syrian hamster model.
One group of convalescent (previously infected with isolate USA-WA1/2020) and one group of naive hamsters (n = 12 per group, age paired) were intranasally inoculated with 10 4 PFU of a U.S. B.1.1.7 variant (isolate CA_CDC_5574/2020, termed CA B.1.1.7). A third group of 12 naive hamsters (n = 12) were similarly infected with a B.1 variant (isolate New York-PV08410/2020, termed NY B.1). The circulating neutralizing antibody titers in the 12 convalescent hamsters ranged from 80 to 320 at the time of challenge (Table 1), determined by a pseudovirus neutralization assay as previously described (11,12). Nasal wash (NW) samples taken from infected hamsters on days 1, 2, and 3 postinfection (p.i.) showed that all hamsters contained detectable subgenomic RNA (sgRNA) levels in the nasal cavities on the first 2 days p.i. (Fig. 1A). Three days following infection, viral sgRNA levels from nasal washes of convalescent hamsters declined to below the detection limit, indicating that convalescent hamsters quickly controlled B.1.1.7 replication just as they controlled the reinfection with homologous viruses (11,13,14). In contrast, naive hamsters that were challenged with either the B.1.1.7 or B.1 variant showed 2-to 3-log 10 -higher levels of sgRNA than the convalescent group. Furthermore, naive hamsters that were challenged with the B.1.1.7 variant displayed overall higher levels of sgRNA in NW samples (statistically significant at day 2 p. i.) than those of the NY B.1-infected group. The same NW samples were also quantified for infectivity by a 50% tissue culture infectious dose (TCID 50 ) endpoint dilution assay (Fig. 1B). Only 3 out of 12 NW samples from convalescent hamsters exhibited detectable levels of infectious virus at day 1 p.i., dropping to below detection at day 2 p.i. (Fig. 1B). Surprisingly, NW samples from NY B.1-infected hamsters contained significantly higher TCID 50 values than those from CA B.1.1.7-infected hamsters on days 2 and 3 p.i. (Fig. 1B). To track the viral loads in the lung, we performed RNAscope on fixed lung tissues collected at 2 days postinfection (dpi), 4 dpi, and 7 dpi using a probe recognizing both NY B.1 and CDC B.1.1.7 variants (see Fig. S1 in the supplemental material). After quantification using Image J software, it was noted that NY B.1 viral RNA levels were higher than the CDC B.1.1.7 variant in the lung, at least at 2 and 7 dpi (Fig. S2). This observation suggests that the B.1.1.7 variant does not display fitness advantage over the B.1 variant in hamster lungs. Convalescent hamsters experienced no weight loss compared to the naive infected groups over the course of 7 dpi (Fig. 1C). Both the CA B.1.1.7-and NY B.1-infected animals lost 10 to 15% of body weight by day 7 p.i. At day 7 p.i., infected hamster lungs displayed pathology including alveolar wall thickening, airway infiltrates, perivascular edema, and hyperplasia ( Fig. 1D and E). However, convalescent hamsters that were subsequently reinfected did not show these signature pathologies in the lungs (Fig. 1E). The cumulative pathology score and percentage of lung consolidation in the CA B.1.1.7 and NY B.1 naive groups had no significant difference (Fig. 1D), but both had significantly higher scores than those of convalescent hamsters (Fig. 1D). Therefore, although B.1.1.7 SARS-CoV-2 variant replication was higher in the nasal cavity on days 1 and 2 p.i. compared to the NY B.1 variant measured by sgRNA, the associated pathology was not more severe in the lung.
To account for the discrepancy between sgRNA titers and TCID 50 titers in NW samples, a second study using 10 male Syrian hamsters was subsequently performed. This study included two convalescent hamsters that were subsequently infected by CA B.1.1.7 and four naive NY B.1-and four naive CA B.1.1.7-infected Syrian hamsters aged .10 months. Again, NW samples of the CA B.1.1.7-infected hamsters show overall higher levels of sgRNA but slightly lower levels of infectious virus than those of NY B.1-infected animals ( Fig. 2A and B). Tissue samples taken from convalescent hamsters at 4 dpi revealed 3-to 4-log 10 -lower levels of sgRNA in all five lobes of the lung, trachea, and nasal turbinate (NT) in comparison to those from naive hamsters challenged with either the CA B.1.1.7 variant or the NY B.1 variant (Fig. 2C). Again, the degree of reduction in viral loads is comparable to what was observed in homologously rechallenged convalescent hamsters (11). Lung and NT homogenates were also titrated by plaque-forming assay in Vero E6 cells (Fig. 2D). Homogenates taken from convalescent hamsters contained no detectable infectious virus We and others have previously demonstrated that prior SARS-CoV-2 infections induced protective immunity in Syrian hamsters against a homologous rechallenge (11,13,14). This study finds that convalescent hamsters are protected against interstitial pneumonia following B.1.1.7 variant rechallenge and that the SARS-CoV-2 B.1.1.7 variant does not appear to be more pathogenic in adult male Syrian hamsters than the B.1 variant (D614G). Notably, breakthrough infections have now been observed in the nose on day 1 or 2 postchallenge with either a homologous (11) or a heterologous SARS-CoV-2 isolate (this study) in a small percentage of the convalescent hamsters despite the lung being completely protected from active viral replication. Difference in viral fitness may exist between the B.1.1.7 and B.1 variants in human beings; however, it was not conclusively observed in Syrian hamsters under the current experimental setting. On one hand, nasal wash samples from hamsters infected by a B.1.1.7 variant exhibited slightly higher viral RNA titers, which suggests higher levels of virus replication in the nasal cavity. On the other hand, infectious titers from the same nasal wash samples or lung homogenates of B.1.1.7 variant-infected animals were noticeably lower than those from B.1 (G614) variant-infected hamsters, arguing that Syrian hamsters may not fully recapitulate the B.1.1.7 infection in humans. Such a discrepancy of viral replication and infectious particles may be attributed to a disadvantaged B.1.1.7 plaquing effect in Vero E6 cells which has been previously mentioned (15), which would lead to undercounting of the B.1.1.7 variant in Vero E6 cells. To determine whether the B.1.1.7 variant undergoes deleterious changes in hamsters that would alter the overall infectivity of the virus, we also performed next-generation sequencing SARS-CoV-2 pseudovirus production and neutralization assay. Human codonoptimized cDNA encoding SARS-CoV-2 S glycoprotein (NC_045512) was synthesized by GenScript and cloned into eukaryotic cell expression vector pcDNA 3.1 between the BamHI and XhoI sites. Pseudovirions were produced by cotransfection of Lenti-X 293T cells with psPAX2, pTRIP-luc, and SARS-CoV-2 S-expressing plasmid using Lipofectamine 3000. The supernatants were harvested at 48 and 72 h posttransfection and filtered through 0.45-mm membranes. For the serum neutralization assay, 50 ml of SARS-CoV-2 S pseudovirions was preincubated with an equal volume of medium containing serum at various dilutions at room temperature for 1 h, and then virus-antibody mixtures were added to 293T-hACE2 cells in a 96-well plate. After 3 h of incubation, the inoculum was a The numbers in parentheses (highlighted in bold) indicate the number of samples that contain the mutation. For example, (6/8) indicates that 6 out of 8 samples contained this mutation.
replaced with fresh medium. Cells were lysed 48 h later, and luciferase activity was measured using luciferin-containing substrate. Controls included cell-only control, a control consisting of virus without any antibody, and positive-control sera. The endpoint titers were calculated as the last serum dilution resulting in at least 50% SARS-CoV-2 neutralization. The amount of pseudovirions used in this assay has been determined to give rise to a target input of 5 Â 10 5 to 5 Â 10 7 relative light units (RLU)/ml, under which condition the neutralization law is observed.
Hamster challenge experiments. Procedures for hamster challenge experiments were as described previously (11,12). For challenge studies, aged (10 to 12 months old) Syrian hamsters were anesthetized with 3 to 5% isoflurane. Intranasal inoculation was done with 10 4 PFU/hamster of SARS-CoV-2 in a 50-ml volume dropwise into the nostrils. Nasal washes were collected by pipetting ;200 ml sterile phosphate-buffered saline into one nostril. Male hamsters (n = 12) were divided into three groups. The first group contained hamsters who were previously infected with the USA-WA1/2020 variant (lineage A, GISAID clade S), which was circulating in Washington State in early 2020. The second group of hamsters were immunologically naive and were intranasally infected with the B.1.1.7 variant hCoV-19/USA/CA_CDC_5574/2020 (GISAID clade GR). The third group of hamsters were challenged with New York-PV08410/2020 (G614, B.1 lineage, GISAID clade GH). Following infection, hamsters were monitored for clinical signs and weight loss. Nasal wash samples taken on days 1, 2, and 3 postinfection (p.i.) to test for sgRNA and TCID 50 . Seven days following infection, a subset of hamsters was humanely euthanized and lungs were taken for histopathology.
Next-generation sequencing. Detailed procedures have been published previously (12). To prepare sequencing libraries, 20 ml RNA was extracted from 140 ml virus stock or hamster lung homogenates. RNA quality was assessed by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA integration numbers (RIN) were all greater than 9. A total of 8.5ml of the samples was used to prepare the sequencing library (2 ml was used to check the RNA quality and size, and 6.5 ml was used for the library construction). Library preparation followed "Illumina section 2 c) Illumina shotgun metagenomics" (https://github.com/CDCgov/SARS-CoV-2_Sequencing). If the RNA was derived from virus stocks, approximately ,19% of total reads were mapped to African green monkey cells (the host cell) even without depletion of rRNA. Therefore, omission of the rRNA removal still yielded enough reads mapped to SARS-CoV-2 (;50 to 75% of total reads). In the Illumina protocol, rRNA removal was usually included if the RNA was derived from tissues but was omitted in this study without affecting the quality of the sequence because we have found that the use of the enrichment-based Illumina sample prep kit yielded similar results as when the rRNA removal step (metagenomic approach) was included. The Illumina sample prep kit (enrichment-based) captures several human respiratory viruses (including SARS-CoV-2). Prepared libraries were loaded unto the NextSeq sequencer for deep sequencing. The reference sequence is coronavirus 2 isolate Wuhan-Hu-1 (GenBank accession no. MN908947.3). Variant calling is done by the CLC Genomics Workbench V20 low-frequency variant detection (Qiagen) with the requirement of significance of $5% and minimum frequency of $20%. We got roughly 500,000 to 900,000 reads per virus sample, each read containing 75 bp. If using the SARS-CoV-2 genome (;30,000 bp) to estimate, the coverage was about 1,250Â to 2,250Â, assuming the reads were distributed equally. In practice, we noticed the C-terminal region has more reads than other regions.
Histopathology and RNAscope analyses. Procedures for histology and RNAscope analyses were as described previously (11,12). See Table S1 in the supplemental material for the pathology scoring sheet.
TCID 50 . Procedures for the TCID 50 assay were as described previously (11,12). Plaque assay. Nasal wash samples were 10-fold serially diluted and added to a 6well plate with Vero E6 cells. After 1 h, the mixture was removed and replenished with tragacanth gum overlay (final concentration 0.3%). Cells were incubated at 37°C and 5% CO 2 for 2 days and then fixed with 4% paraformaldehyde, followed by staining of cells with 0.1% crystal violet in 20% methanol for 5 to 10 min.

SUPPLEMENTAL MATERIAL
Supplemental material is available online only. FIG S1, TIF