High-Throughput Screen for Inhibitors of the Type IV Pilus Assembly ATPase PilB

ABSTRACT The bacterial type IV pilus (T4P) is a prominent virulence factor in many significant human pathogens, some of which have become increasingly antibiotic resistant. Antivirulence chemotherapeutics are considered a promising alternative to antibiotics because they target the disease process instead of bacterial viability. However, a roadblock to the discovery of anti-T4P compounds is the lack of a high-throughput screen (HTS) that can be implemented relatively easily and economically. Here, we describe the first HTS for the identification of inhibitors specifically against the T4P assembly ATPase PilB in vitro. Chloracidobacterium thermophilum PilB (CtPilB) had been demonstrated to have robust ATPase activity and the ability to bind its expected ligands in vitro. We utilized CtPilB and MANT-ATP, a fluorescent ATP analog, to develop a binding assay and adapted it for an HTS. As a proof of principle, we performed a pilot screen with a small compound library of kinase inhibitors and identified quercetin as a PilB inhibitor in vitro. Using Myxococcus xanthus as a model bacterium, we found quercetin to reduce its T4P-dependent motility and T4P assembly in vivo. These results validated our HTS as effective in identifying PilB inhibitors. This assay may prove valuable in seeking leads for the development of antivirulence chemotherapeutics against PilB, an essential and universal component of all bacterial T4P systems. IMPORTANCE Many bacterial pathogens use their type IV pili (T4P) to facilitate and maintain infection of a human host. Small chemical compounds that inhibit the production or assembly of T4P hold promise in the treatment and prevention of infections, especially in the era of increasing threats from antibiotic-resistant bacteria. However, few chemicals are known to have inhibitory or anti-T4P activity. Their identification has not been easy due to the lack of a method for the screening of compound collections or libraries on a large scale. Here, we report the development of an assay that can be scaled up to screen compound libraries for inhibitors of a critical T4P assembly protein. We further demonstrate that it is feasible to use whole cells to examine potential inhibitors for their activity against T4P assembly in a bacterium.

IMPORTANCE Many bacterial pathogens use their type IV pili (T4P) to facilitate and maintain infection of a human host. Small chemical compounds that inhibit the production or assembly of T4P hold promise in the treatment and prevention of infections, especially in the era of increasing threats from antibiotic-resistant bacteria. However, few chemicals are known to have inhibitory or anti-T4P activity. Their identification has not been easy due to the lack of a method for the screening of compound collections or libraries on a large scale. Here, we report the development of an assay that can be scaled up to screen compound libraries for inhibitors of a critical T4P assembly protein. We further demonstrate that it is feasible to use whole cells to examine potential inhibitors for their activity against T4P assembly in a bacterium.
KEYWORDS PilB ATPase, type IV pili (T4P), high-throughput screen (HTS), antivirulence, quercetin, motility, Myxococcus xanthus T he bacterial type IV pilus (T4P), a protein polymer comprised of thousands of pilins (1), is a virulence factor in many pathogens (2)(3)(4) and a target for potential chemotherapeutics for disease intervention (5). The T4P filament can extend several micrometers from the cell body and is primarily used as an adhesin during the infection cycle by a bacterial pathogen (2)(3)(4)6). As a virulence factor, the T4P facilitates the adherence of a bacterium to the surface of host cells or a medical device to initiate infections (6)(7)(8). In some cases, this leads to the development of biofilms, which can transition an acute infection to a chronic one (9)(10)(11). There is also evidence that the physical contact of T4P with host cells or surfaces can regulate the expression of other virulence factors (9,(11)(12)(13)(14). The loss of T4P has been shown to significantly attenuate or compromise the virulence process of pathogenic bacteria such as Acinetobacter baumannii, Clostridioides difficile, pathogenic Escherichia coli, Francisella tularensis, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Vibrio cholerae (3,4,(15)(16)(17)(18). Because the T4P is integral to the infection process for a diverse range of bacterial pathogens, it provides a potential target for the development of therapies against their infections.
The assembly of pilins into the T4P filament requires the T4P machinery (T4PM), which is comprised of a dozen highly conserved T4P or Pil proteins (19). Among them is the cytoplasmic PilB ATPase, commonly known as the T4P assembly or extension motor (20,21). It hydrolyzes ATP to power the incorporation of pilins into a growing pilus at the base of the T4PM. PilT, another ATPase present in many (but not all) T4P systems, is known as the T4P disassembly or retraction motor (20). It uses ATP as the energy source to disassemble T4P by retraction. The recurrent cycles of T4P extension and retraction catalyzed by PilB and PilT can result in motility in bacteria such as Neisseria, P. aeruginosa, and Myxococcus xanthus (22)(23)(24). This form of motility is known as bacterial twitching or M. xanthus social (S) gliding (23,25). A lack of PilB results in the absence of T4P, while elimination of PilT leads to hyperpiliation because of defects in T4P retraction (20,26). As a result, pilB as well as pilT mutants have no T4P-dependent motility, providing a convenient assay for the functionality of the T4PM in bacteria with this form of motility (20,26).
Antivirulence compounds are small molecules that inhibit or interfere with the function or expression of virulence factors such as the T4P (27,28). As far as we are aware, there have been only two reports of anti-T4P compounds in the literature, both of which appeared in 2019 (29,30). In one case (29), the antipsychotic drug trifluoperazine and other related phenothiazines were serendipitously discovered because they induced the dispersion of Neisseria meningitidis microaggregates by decreasing T4P levels. Using primary endothelial cells and brain vessels of human origin as well as a humanized mouse model, these compounds were found to reduce bacterial colonization as well as bacterially induced cell injury and vascular lesions (29). In a mouse infection model, phenothiazines were found to provide adjunctive benefits when administered alongside antibiotics (29). Genetic studies identified the target of these anti-T4P compounds as the Na 1 pumping NADH-ubiquinone oxidoreductase (Na 1 -NQR) complex, which was not known to be involved in T4P dynamics prior to this report (29). In the other case (30), P4MP4 {1-[(piperidin-4-yl)methyl]piperidin-4-ol} (5) was identified from a library of 2,239 compounds by a high-throughput screen (HTS) based on the reduction of N. meningitidis adhesion to cultured cells. Coincidentally, P4MP4 affected N. meningitidis aggregation and T4P in a manner resembling that of phenothiazines despite their different routes of discovery. Biochemical studies in this investigation pointed to PilF, the PilB equivalent in the N. meningitidis T4P system, as the potential target of P4MP4 instead of the Na 1 -NQR complex (30). It should be emphasized that the T4PM was not specifically targeted by the antiaggregation (29) or the antiadhesion (30) assays in either case for the discovery of phenothiazines or P4MP4. The convergence of their effects on the T4P strongly substantiated the T4PM as a valuable target for the development of antivirulence chemotherapeutics (5).
We report here the development of an HTS for the identification of compounds that inhibit the PilB ATPase specifically. Our previous work with Chloracidobacterium thermophilum PilB (CtPilB) showed that it is a robust ATPase (31) that binds the secondary messenger c-di-GMP with a critical role in biofilm regulation (32). Here, we demonstrate that the fluorescent ATP analog MANT-ATP also binds CtPilB and that its fluorescence is significantly increased by this association. We developed an assay for the identification of compounds that reduced MANT-ATP binding to CtPilB, thereby causing a decrease in MANT-ATP fluorescence. After optimization and adaptation of this assay for HTS, we identified quercetin from a small-compound library of kinase inhibitors. Biochemical assays confirmed that quercetin inhibits both the binding of CtPilB with MANT-ATP and its ATPase activity in vitro. Experiments using M. xanthus as a model further indicated that quercetin reduced the expansion of M. xanthus colonies by its T4P-dependent S motility. Additional results suggested that the inhibitory effect of quercetin on M. xanthus S motility is related to diminished T4P assembly, supporting the conclusion that quercetin functions as an inhibitor of the PilB assembly ATPase in vivo. These results collectively illustrate that our newly developed HTS is able to identify compounds in vitro that can be effective in vivo to facilitate the development of antivirulence chemotherapeutics against bacterial pathogens with T4P as a virulence factor.

RESULTS
ATP and its fluorescent analog MANT-ATP compete for binding to PilB. CtPilB was used previously to analyze its binding of ATP, ADP, and ATP-g-S by isothermal calorimetry (ITC) (32). An alternative for such analysis is to use fluorescent nucleotide analogs, such as MANT-ATP, which may emit enhanced fluorescence when bound to proteins (33,34). We examined the binding between CtPilB and MANT-ATP as described in Materials and Methods (M&M). As shown in Fig. 1A, increasing concentrations of CtPilB led to increased fluorescence of MANT-ATP, which was kept at a constant concentration. A binding isotherm fitted to this data set produced a dissociation constant (K D ) of 0.29 6 0.01 mM for the binding of these two partners. These results indicate that the fluorescence of MANT-ATP can be used to analyze its binding with CtPilB.
The above K D value of 0.29 mM for MANT-ATP and CtPilB binding is approximately 10-fold lower than the K D of 3.8 mM for that between ATP and CtPilB determined by ITC (32). This increase in affinity for an ATP analog was similarly observed between protein kinases and TNP-ATP (35,36), possibly due to additional interactions of the fluorophore of a fluorescent ATP analog with the protein (37). If MANT-ATP occupies the orthosteric pocket as ATP as expected, it should behave as a competitive ligand or inhibitor. If so, the K D for ATP could be determined by its inhibition constant (K i ) on MANT-ATP and CtPilB binding. As shown in Fig. 1B, when ATP was included in reaction mixtures containing both MANT-ATP and CtPilB, the fluorescent signal decreased with increasing concentrations of ATP. The half maximal inhibitory concentration (IC 50 ) of ATP on MANT-ATP binding was determined to be 3.15 mM. The resulting K i is 2.20 mM, close to the previously published K D value (32). Likewise, ADP also competed with MANT-ATP for CtPilB binding as indicated by the decline in the fluorescence signal with increasing ADP concentrations (inset in Fig. 1B). The ability of both ATP and ADP to compete with MANT-ATP agrees with previously published observations, which showed that both nucleotides bind to CtPilB with similar affinities (32). AMP up to 100 mM failed to compete with MANT-ATP for CtPilB binding (data not shown). This is consistent with observations that both the gand b-phosphates of ATP are crucial for interactions with PilB (21,38,39). In summary, these results show that MANT-ATP can be used as a fluorescent probe to analyze the binding of CtPilB to its cognate nucleotide ligands.
Development and implementation of an HTS identified quercetin as a PilB inhibitor. We reasoned that if a compound could reduce the binding of ATP to PilB, it would inhibit the ATPase activity of PilB. Such an inhibitor could be identified from a compound library by HTS based on its ability to reduce the binding of MANT-ATP to CtPilB. ATP, which clearly competes with MANT-ATP for CtPilB binding (Fig. 1B), was used as a positive control to develop an HTS in a 384-well microtiter plate format. The HTS included 0.4 mM MANT-ATP and 0.5 mM CtPilB in a total volume of 20 ml per well. Using this assay, we screened a Selleckchem compound library of 273 kinase inhibitors. For the library compounds, a final concentration of 20 mM was used for the HTS. Included in the screen were four sets of controls, each in multiple wells at different positions on the screen plate and all with the same concentration of dimethyl sulfoxide (DMSO) as the compound wells. The first set, the positive control, contained CtPilB and MANT-ATP with ATP as the inhibitory or competitive ligand. The second set, the negative controls, contained CtPilB and MANT-ATP without ATP. The last two, which were used for normalization, contained either CtPilB or MANT-ATP alone. An acceptable Z9 factor (40) of 0.57 was calculated for this HTS. As shown in Fig. 2A, although a few library compounds lowered the fluorescence relative to the negative control, compound 77 stood out in that it decreased the fluorescence to levels similar to those of the positive control with ATP. This compound is quercetin, a flavonoid of plant origin that inhibits the activity of protein kinases (41) as well as ATPases (42,43). These results suggest that our HTS is effective for the identification of PilB inhibitors and that quercetin could inhibit CtPilB as it does other ATPases (42,43).
Quercetin inhibits the ATPase activity of CtPilB in vitro. We examined by biochemical experiments if quercetin could indeed inhibit the binding of CtPilB to MANTP-ATP as described earlier (see Fig. 1B). As shown in Fig. 2B, the fluorescence of MANT-ATP in the presence of CtPilB was clearly diminished by quercetin in a dosagedependent manner. It reduced the fluorescence to the baseline when quercetin was at or above 10 mM. The IC 50 of quercetin was determined to be 2.12 mM, showing that quercetin inhibits the binding of PilB to MANT-ATP with a potency similar to that of ATP, which had an IC 50 of 3.15 mM in this assay (Fig. 1B).
To investigate if quercetin could inhibit the ATPase activity of CtPilB, we analyzed the ATPase activity of CtPilB (31) in the presence of quercetin. As shown in Fig. 2C, the activity of CtPilB declined in the presence of quercetin in a dosage-dependent manner. ATP hydrolysis by CtPilB was completely inhibited by quercetin at or above 32 mM. The IC 50 for this inhibition is estimated to be about 2.50 mM, which is similar to 2.12 mM, the IC 50 for the inhibition of MANT-ATP binding to CtPilB (Fig. 2B). We conclude that quercetin is a potent inhibitor of CtPilB ATPase in vitro, demonstrating that our newly developed HTS is effective in identifying PilB inhibitors from a compound library ( Fig. 2A).
Quercetin impedes T4P-mediated bacterial motility in vivo. We next examined if quercetin has the ability to impact functions associated with T4P in vivo using M. xanthus as a model organism. This bacterium has a form of surface motility that is powered by T4P extension and retraction (23,44). Its T4P-depedent motility, commonly known as S motility, can be conveniently analyzed on plates with low-percentage agar (soft agar) (45). Experiments were conducted with two strains, YZ1674 and YZ2232, both of which produce T4P (T4P 1 ) and exhibit the T4P-dependent S motility (31). YZ1674 expresses the endogenous or wild-type (WT) M. xanthus PilB (MxPilB), while YZ2232 expresses MC 3 PilB, a PilB chimera with the N terminus of MxPilB and the C-terminal ATPase catalytic domains of CtPilB. The pilB deletion (DpilB) strain DK10416 was used as the negative control, as it is devoid of T4P (T4P 2 ) and without S motility. These M. xanthus strains were tested for their T4P-mediated motility on soft agar plates with different concentrations of quercetin. As shown in Fig. 3A and Fig. S1 in the supplemental material, quercetin at or above 32 mM significantly reduced the spreading of the MxPilB-expressing (YZ1674) and the MC 3 PilB-expressing (YZ2232) strains to comparable extents. The results are consistent with earlier observations that quercetin at the same concentrations inhibited both MANT-ATP binding and the ATPase activity of CtPilB ( Fig. 2B and 2C). The results from the motility assays suggest that quercetin is capable of inhibiting the activity of PilB as the T4P assembly ATPase in M. xanthus in vivo.
However, quercetin is also known to inhibit the growth of certain bacteria, including Gram-negative bacteria (46). The observed reduction in M. xanthus S motility described above could be due to the inhibition of growth. We examined the effect of quercetin on M. xanthus growth in liquid culture as shown in Fig. 3B. The results indicated that quercetin could inhibit M. xanthus with an estimated MIC of about 128 mM. At the sub-MICs of 32 mM and 64mM, quercetin also appreciably reduced the growth of M. xanthus in liquid (Fig. 3B). Coincidentally, these are the concentrations at which quercetin affected M. xanthus T4P-dependent or S motility in plate assays as well (Fig. 3A). It is pertinent here to highlight that M. xanthus has long been known to have T4P-dependent and T4P-independent motility systems (23,47,48). While the former can be analyzed on soft agar plates, the latter, known as adventurous (A) motility, can be assayed on plates with 1.5% agar (hard agar) (45). We therefore examined the impact of quercetin on the A motility system of M. xanthus on hard agar plates. As shown in Fig. S2 and S3, none of the three M. xanthus strains had their colony expansion impacted by quercetin up to 64 mM. These results argue that the effect of quercetin on M. xanthus T4P-dependent motility is not likely attributable to an effect on growth. Instead, the inhibition of PilB as the T4P assembly ATPase is a more reasonable explanation for the effect of quercetin on M. xanthus S motility.
Quercetin does not impact the EPS level of M. xanthus. It should be noted that the T4P-mediated motility of M. xanthus requires exopolysaccharide (EPS), in addition to the presence of retractable T4P (49). An agglutination assay was performed as a first step to examine the effect of quercetin on EPS because it is required for M. xanthus to agglutinate (49). As shown in Fig. 4A, quercetin up to 16 mM did not affect M. xanthus agglutination. Although M. xanthus agglutinated in the presence of 32 mM quercetin, this occurred at a noticeably lower rate. When quercetin was increased to 64 mM, M. xanthus failed to agglutinate entirely. Because quercetin inhibited agglutination and T4P-mediated motility at similar concentrations (Fig. 3A), its effect on T4P-dependent motility could be through the modulation of EPS, T4P, or both. We next examined M. xanthus EPS levels more directly by a plate assay based on the binding of the fluorescent dye calcofluor white (CW) by M. xanthus EPS (50). Cells were spotted onto CWcontaining plates with different concentrations of quercetin, and the fluorescence was examined after incubation for 7 days (51). As show in Fig. 4B, no difference in fluorescence was observed regardless of the concentration of quercetin. These observations suggested that quercetin does not impact EPS levels in M. xanthus on plates and its effect on T4P-depedent motility is consistent with an effect on T4P assembly through the inhibition of PilB. These results are reminiscent of the delayed-agglutination phenotype of an M. xanthus strain with elevated c-di-GMP levels through the expression of an exogenous diguanylate cyclase (52); this strain was found to have reduced levels of piliation with wild-type level of EPS.
Quercetin inhibits T4P assembly in M. xanthus. We analyzed the effect of quercetin on T4P assembly more directly using a dot blotting assay under nongrowing conditions (see M&M). In this assay, the pili on M. xanthus were first sheared off mechanically by vortexing cells in a buffer without nutrients (53,54). These "bald" cells were then allowed to repiliate in the absence or presence of quercetin in the buffer for 20 min. For each treatment, three fractions were prepared and analyzed using anti-pilin antibodies. The T4P fraction contained the sheared pilus as a measure of piliation levels, whereas the "pilin" fraction was the lysate of cells with their pili sheared off. Also included was the whole-cell (WC) lysate from cells with their pili intact as a control. During the protocol development, bald M. xanthus cells were found to restore their piliation near preshearing levels in about 30 min under our experimental conditions (Fig. S4). For later experiments, incubation with quercetin was allowed for 20 min before stoppage of the treatment.
As shown in Fig. 5A, the signal strength for pilins in the WC and pilin fractions were not affected by the treatment with quercetin, which was confirmed by quantification of the dot blot (Fig. 5B). In contrast, the levels of piliation as indicated by the T4P fraction were clearly reduced by quercetin in a concentration-dependent manner. The signal for T4P in the presence of 16 mM quercetin was not statistically different from that without quercetin. However, the 20-min treatment with 32 mM and 64 mM quercetin significantly reduced piliation levels. At these two higher quercetin concentrations, T4P levels were about 50% and 30% of that without quercetin treatment, respectively (Fig. 5B).
The above-described treatments were conducted in a buffer with no nutrients for bacterial growth in a short time frame. However, because quercetin at 32 mM and 64 mM appreciably decreased the growth of M. xanthus in liquid media over a longer time span (Fig. 3B), it was possible that the quercetin treatment described above, however brief, could impact cell viability or membrane integrity. We used LIVE/DEAD staining to examine the effect of quercetin treatment on cells under the same conditions as for the above-described piliation assay (Fig. 5). As shown in Fig. S5, in the control samples, in which no quercetin was included, 95.7% 6 1.4% cells were found to be viable. The proportions of viable cells treated with 16 mM, 32 mM, and 64 mM quercetin were virtually identical to that of the control at 96.4% 6 0.7%, 96.7% 6 0.8%, and 96.1% 6 0.6%, respectively. These observations show that the effect of quercetin on T4P assembly under our experimental conditions (Fig. 5) is not due to an influence on cell viability or membrane integrity.
Taking into consideration that quercetin was initially identified as an inhibitor of the PilB ATPase in vitro (Fig. 2), the observations in this experiment (Fig. S5) and the differential motility assays (Fig. 3) support the conclusion that the inhibition of T4P assembly by quercetin (Fig. 5) likely results from its inhibition of T4P assembly in vivo through a direct effect on PilB as the T4P assembly ATPase.

DISCUSSION
Here, we describe the development of an HTS that allowed the identification of quercetin as an anti-T4P compound targeting the assembly ATPase PilB. This work took advantage of CtPilB, a member of the PilB ATPase family that is amenable to in vitro analysis. We utilized its binding to a fluorescent ATP analog to develop an assay adapted for HTS. This led to the discovery and confirmation of quercetin as an inhibitor of CtPilB ATPase activity in vitro. Using M. xanthus as a model organism, we demonstrated that quercetin inhibits T4P-dependent motility and T4P assembly in vivo. We conclude that our HTS can be effective in the identification of PilB inhibitors from compound libraries for the development of chemotherapies against bacteria with T4P as a virulence factor. It should be noted that in this HTS, about 25% of the library compounds fluoresced at a wavelength similar to that of MANT-ATP ( Fig. 2A). Therefore, their ability to inhibit MANT-ATP binding to CtPilB could not be assessed.
With the increasing prevalence of antibiotic resistance in bacterial pathogens, there is a pressing need to explore treatments of bacterial infections other than the use of antibiotics. Antivirulence therapies, which target bacterial virulence factors, are considered a promising alternative. Passive immunotherapies against virulence factors such as the T4P have been explored in animal models (55,56). Interference with or inhibition of the expression or function of virulence factors by small antivirulence molecules is another approach in the antivirulence strategy (57). Yet chemicals with anti-T4P activities have been few and far between, especially in comparison with those targeting type III secretion systems and bacterial quorum sensing systems (58)(59)(60)(61). There had been no knowledge of anti-T4P small molecules before the reports of phenothiazines and P4MP4 in 2019 (29,30). One of the main challenges was the lack of an HTS for the identification of inhibitors of the T4PM. The discovery of phenothiazines stemmed from the fortuitous observation that the antipsychotic drug trifluoperazine dispersed N. meningitidis cells in microaggregates (29). While P4MP4 was identified in an HTS, the assay focused on the inhibition of N. meningitidis adhesion to cultured cells with extensive imaging requirements (30). These topdown approaches did not target the T4PM specifically and could well have led to targets other than the conserved T4P proteins.
Our assay takes more of a bottom-up approach to specifically target the ubiquitous T4P protein PilB. We used CtPilB as a model enzyme to screen for inhibitors because it is the most active among the canonical PilB ATPases in vitro (31). The use of a model protein for inhibitor identification was validated in this study in two M. xanthus strains expressing different PilB variants. One strain (YZ1674) has the wild-type endogenous MxPilB, while the other (YZ2232) has MC 3 PilB, a hybrid PilB with the ATPase catalytic core of CtPilB (Fig. 3). It was perhaps not surprising that quercetin inhibited the T4P-dependent motility of YZ2232 (MC 3 PilB). More importantly, the T4P-dependent motility of YZ1674 (MxPilB) was similarly inhibited, suggesting that quercetin may interact with and inhibit MxPilB and CtPilB in similar fashions mechanistically. Additional experiments with M. xanthus further substantiated the conclusion that quercetin negatively impacted T4P assembly in this bacterium because quercetin reduced T4P levels on cells with preexisting T4P sheared off. In this context, it is noted that quercetin was reported to inhibit twitching in P. aeruginosa (62). In combination with the observations that phenothiazines and P4MP4 were active in both N. meningitidis and N. gonorrhoeae (5,30), these results lend credence to the use of CtPilB as a model protein to identify leads for the development of anti-T4P chemotherapeutics against pathogenic bacteria with orthologous PilB proteins.
In summary, we have developed the first known HTS that specifically targets a T4P protein to allow the identification of anti-T4P compounds by a bottom-up approach. This is possible thanks to the availability of CtPilB as a representative of the conserved family of PilB ATPases. The rationale here was that PilB enzymes from most bacteria examined up to date have been recalcitrant to productive biochemical and biophysical analysis in vitro, with CtPilB as an exception. It has been demonstrated to be a hexameric ATPase with expected ligand binding capacity and robust ATP hydrolyzing activities (31,32). The results here and elsewhere support the use of a model protein for the discovery and development of anti-T4P compounds for antivirulence purposes. There is no question that this principle has been applied to the development of antibiotics such as b-lactams against diverse bacteria with phenomenal success.
All stock solutions of quercetin used in this study were prepared by dissolving quercetin hydrate (ACROS Organics) in dimethyl sulfoxide (DMSO; Fisher Biotech). Unless otherwise stated, 10Â stocks were prepared for each concentration of quercetin in DMSO before their use in experiments. Controls without quercetin had the same concentration of DMSO as those with quercetin. The Selleckchem kinase library L1200 used for the HTS had been reformulated to be 1 mM stocks in DMSO.
GraphPad Prism v 7.04 was used for curve fitting and data analysis. Student's t test was used for statistical analysis.
Biochemical methods. To analyze the binding between CtPilB and MANT-ATP, MANT-ATP at 0.20 mM was mixed with CtPilB at various concentrations in a 96-well plate in CtPilB activity buffer (31). Samples were incubated at room temperature for 5 min before they were analyzed for fluorescence using an Infinite F200 PRO plate reader. The excitation wavelength (l ex ) was set to 355 nm and the emission wavelength (l em ) to 448 nm. For data analysis, the fluorescence of MANT-ATP and CtPilB by themselves were subtracted for normalization. Their dissociation constant (K D ) was calculated by fitting data to the Hill equation with the concentration of MANT-ATP and the fluorescence values as the variables. To analyze the competition with MANT-ATP for CtPilB binding, ATP and ADP at different concentrations were mixed with CtPilB at 0.50 mM and MANT-ATP at 0.20 mM in triplicates. The mixtures were incubated for 5 min at room temperature, and the fluorescence signals were measured and normalized by subtracting the fluorescence measured for CtPilB and MANT-ATP. The IC 50 of ATP and ADP on MANT-ATP binding were calculated using the 4-parameter logistic model (64) with the fluorescence and ATP or ADP concentrations as the variable. The inhibition constant (K i ) was calculated using the Cheng-Prusoff equation (65).
To examine the effect of quercetin on the binding between MANT-ATP and CtPilB, reactions with CtPilB at 0.25 mM and MANT-ATP at 4 mM in triplicates were incubated at room temperature in the presence of quercetin at the indicated concentrations. Fluorescence was measured and normalized as described above. To analyze the effect of quercetin on the ATPase activity of CtPilB, an endpoint ATPase assay was performed as previously described (31). The IC 50 of quercetin on both MANT-ATP binding and ATPase activity were calculated as described above (64) with the concentrations of quercetin and either the fluorescence or the ATPase activity as variables.
HTS based on fluorescence competition assay. The HTS was conducted at the Virginia Tech Center for Drug Discovery (VTCDD) Screening Laboratory using the Agilent Bravo automated liquid-handling platform with 384-well assay plates (Greiner; 781101). Each assay well contained CtPilB at 0.5 mM, MANT-ATP at 0.4 mM, and a library compound at 20 mM in 20-ml reaction mixtures in activity buffer. Fluorescence was measured using a SpectraMax M5 multimode microplate reader at room temperature. For the development and optimization of the HTS, ATP at 20 mM was used as the positive control because it was viewed as an inhibitor of the binding between MANT-ATP and CtPilB, whereas samples without ATP were the negative controls for the absence of an inhibitor. The Z9 factor for this assay was calculated using the following equation (40): Z9 = 1 2 [3 Â (SD 1 1 SD 2 )/(m 1 2 m 2 )], where SD is the standard deviation and m is the fluorescence intensity. The plus and minus subscripts refer to MANT-ATP and MANT-ATP with CtPilB, respectively.
Examination of the effects of quercetin on the bacterium M. xanthus. Plate-based assays were used to examine motility and EPS levels. The starting materials for all experiments with M. xanthus were cells grown to log phase in CYE liquid media. M. xanthus cells were harvested and resuspended in MOPS buffer (10 mM morpholinepropanesulfonic acid [pH 7.6] and 2 mM MgSO 4 ) to an optical density at 600 nm (OD 600 ) of 5 (;5 Â 10 9 cells/ml). For motility assays, 5 ml of the cell suspension was spotted onto either soft (0.4% agar) or hard (1.5% agar) CYE agar plates with the desired concentrations of quercetin. After incubation at 32°C for 4 days, the diameters of the colonies on these two sets of plates were examined to analyze T4P-depdendent and T4P-independent motility (45), respectively. In total, 12 colonies were measured per strain per set of conditions. To examine EPS levels, 5-ml aliquots of the cell suspension were placed onto CYE plates (1.5% agar) with calcofluor white (50 mg/ml) and EPS levels were assessed by fluorescence as previously described (51).
Liquid cultures were used to determine the MIC of quercetin and to examine the agglutination of M. xanthus cells. For the former, cells of YZ1674 were used to inoculate 5-ml CYE cultures with and without quercetin to a final OD 600 of 0.05. These cultures were incubated in an orbital shaker-incubator at 32°C and 300 rpm for 24 h before their OD 600 s were measured. Cell agglutination was performed as described previously (26), with slight modifications. Briefly, cells were harvested, washed three times, and resuspended in agglutination buffer (MOPS buffer described above with 1 mM CaCl 2 ). Cell suspensions were vortexed at the highest setting for 2 min to shear off their pili (53,54). These bald cells were pelleted by centrifugation and resuspended in agglutination buffer to an OD 600 of 0.9 with or without quercetin. Cell suspensions were transferred to cuvettes for the monitoring of OD 600 over a time span of 3 h.
To assess the effect of quercetin on piliation, the pili on cells of M. xanthus strain YZ603 were sheared off as described above (53,54). Bald cells were resuspended to an OD 600 of 1 in ice-cold agglutination buffer with or without quercetin. After a 10-min pre-equilibrium period on ice, samples were incubated at 32°C for 20 min in the dark. Samples were then chilled on ice and centrifuged at 7,500 Â g at 4°C for 10 min. These samples were vortexed for 2 min at the highest setting, followed by centrifugation at 16,000 Â g for 4 min at 4°C. The pellets, which contained cells with their T4P sheared off, were resuspended to an OD 600 equivalent of 5 in lysis buffer (50 mM Tris-HCl [pH 6.8], 2% [mass/vol] sodium dodecyl sulfate, and 1% [vol/vol] b-mercaptoethanol) as the pilin fraction. The supernatants, which contained the sheared-off T4P, were treated with MgCl 2 at a final concentration of 100 mM for 1 h on ice. The T4P in the supernatant were precipitated by centrifugation at 21,000 Â g for 15 min at 4°C. The resulting pellet was suspended to an OD 600 equivalent of 10 with the lysis buffer as the T4P fraction. A lysate from whole cells (WC) with their T4P intact was also prepared in the lysis buffer with an OD 600 equivalent of 5 for comparison. All above-described samples in the lysis buffer were boiled for 10 min and stored for future analysis.
For dot blotting using anti-pilin antibodies, a protocol was developed based on a previously described procedure (66). Briefly, the above-described WC and the pilin fractions were diluted 10fold and the T4P fractions were diluted by a factor of 2. Four 1-ml aliquots of a sample were spotted onto a nitrocellulose membrane in a row and air dried. After blocking with blocking buffer (50 mM Tris base [pH 7.5], 150 mM NaCl, 5% [mass/vol] nonfat dry milk, and 0.1% [vol/vol] Tween 20), the membrane was incubated with rabbit anti-M. xanthus PilA serum (67) at a 1:10,000 dilution in TBST buffer (same as blocking buffer except with 0.1% [mass/vol] nonfat dry milk). This was followed by incubation with goat anti-rabbit antibodies conjugated to horseradish peroxidase (Thermo Fisher) at a 1:15,000 dilution in the same buffer. Blots were developed using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher). The chemiluminescence signal was captured with a 1.5-s exposure using a ChemiDoc MP imaging system (Bio-Rad). For quantification, the pixel densities of the samples were analyzed using ImageJ (68).

SUPPLEMENTAL MATERIAL
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