Construction of Candida albicans Strains with ATP-Analog-Sensitive Protein Kinase A and Hog1

ABSTRACT Candida albicans is an opportunistic human fungal pathogen and a member of the mucosal microbiota. To survive in the host and cause disease, C. albicans utilizes several virulence traits, including the ability to respond and adapt to diverse stressors, as well as the morphogenetic switch between yeast and filamentous morphologies. While complex cellular circuitry governs these virulence attributes, the following two kinase-mediated signaling pathways play particularly critical roles in controlling these processes: the Hog1 mitogen-activated protein kinase (MAPK) cascade and the protein kinase A (PKA) pathway. Here, we describe the construction of C. albicans strains harboring substitutions in the ATP-binding pockets of Hog1 and the catalytic subunits of PKA, Tpk1, and Tpk2 to render their activities sensitive to the addition of bulky ATP analogs. Specifically, inhibition by the ATP analog 1NM-PP1 resulted in phenotypes characteristic of the corresponding homozygous deletion mutants for each kinase gene. These strains represent a toolset for the rapid and specific inhibition of PKA and Hog1 kinase activity to further understand their roles in regulating C. albicans morphogenesis and stress responses. IMPORTANCE As an opportunistic pathogen in humans, the fungus Candida albicans relies on virulence traits to cause disease. They include the ability to transition from yeast to filamentous morphologies and the ability to grow in diverse environmental stress conditions, including nutrient limitation, as well as osmotic and heat shock. Previous work identified the following two kinases that play a critical role in regulating these responses: Hog1 and PKA. Here, we generated versions of each kinase that are sensitive to inhibition by a bulky ATP analog, 1NM-PP1. In the presence of the analog, kinase activity is inhibited rapidly and specifically, facilitating the analysis of both kinases in regulating C. albicans morphogenesis and stress responses. Together, these strains represent an important toolset to further our understanding of C. albicans biology and virulence.

IMPORTANCE As an opportunistic pathogen in humans, the fungus Candida albicans relies on virulence traits to cause disease. They include the ability to transition from yeast to filamentous morphologies and the ability to grow in diverse environmental stress conditions, including nutrient limitation, as well as osmotic and heat shock. Previous work identified the following two kinases that play a critical role in regulating these responses: Hog1 and PKA. Here, we generated versions of each kinase that are sensitive to inhibition by a bulky ATP analog, 1NM-PP1. In the presence of the analog, kinase activity is inhibited rapidly and specifically, facilitating the analysis of both kinases in regulating C. albicans morphogenesis and stress responses. Together, these strains represent an important toolset to further our understanding of C. albicans biology and virulence.
KEYWORDS 1NM-PP1, ATP, Candida albicans, Hog1, protein kinase A, genetic resource I n humans, fungal pathogens are responsible for over 1 billion infections and 1.5 million deaths annually (1). The majority of infections in humans are caused by Candida albicans, an opportunistic pathogen that is a commensal organism in approximately half of the global population (2). C. albicans pathogenesis relies on several key virulence strategies that contribute to disease and immunopathology in human hosts, including the ability to transition from yeast to filamentous morphologies, such as hyphae (3). A core signaling pathway responsible for regulating the yeast-to-filament transition in C. albicans is the cyclic-AMP (cAMP)-protein kinase A (PKA) pathway (3). Specifically, activation of the adenylyl cyclase Cyr1 to produce cAMP relieves the PKA catalytic subunits Tpk1 and Tpk2 from inhibition by the regulatory subunit Bcy1. PKA signals are transduced to multiple transcription factors to drive filamentation. Another kinase important for C. albicans virulence is the mitogen-activated protein kinase (MAPK) Hog1, which mediates responses to diverse stressors (4)(5)(6)(7). Hog1 also regulates hyphal morphogenesis, as well as the interaction of C. albicans with host phagocytes (8,9). Homozygous deletion and conditional expression mutants have been used to study loss of PKA or Hog1 function in C. albicans. However, genetic methods now exist to allow for rapid and specific inhibition of protein kinases by ATP analogs (10,11). This strategy involves mutating a gatekeeper residue in a kinase to render the ATPbinding pocket accessible to bulky ATP-analogs, such as C3-1'-naphthyl-methyl PP1 (1NM-PP1). Binding of this analog competes with the binding of ATP and renders the kinase inactive. While this approach has been used to study the function of particular kinases in C. albicans, including protein kinase C (Pkc1) (12), Cdc28 (13,14), Ssn3 (15), and Yak1 (16), it has not been used to study the function of PKA or Hog1.
Here, we first describe the construction and characterization of analog-sensitive (AS) versions of the catalytic subunits of PKA in C. albicans. To do so, we constructed vectors containing wild-type (WT) TPK1 or TPK2 alleles. M170G or M180G substitutions were then introduced into the TPK1 or TPK2 constructs, respectively, based on the fact that equivalent mutations in Saccharomyces cerevisiae Tpk proteins confer 1NM-PP1 sensitivity (17). The WT and AS alleles were then used to complement the corresponding homozygous deletion mutant at the native loci. Native TPK2 (or TPK1) was then deleted to ensure that the reintroduced WT or AS TPK1 (or TPK2) alleles were the sole source of PKA activity in each strain (Fig. 1A). The C. albicans tpk1 tpk2 homozygous deletion mutant exhibits a significant growth defect, despite being viable (18). To functionally characterize our newly constructed strains, we monitored growth in the absence and presence of 1NM-PP1. In the absence of 1NM-PP1, WT or AS alleles of TPK1 or TPK2 supported normal growth in rich medium (yeast extract-peptone-dextrose [YPD]) (Fig. 1B). The addition of 1NM-PP1 inhibited the growth of strains expressing either TPK1 or TPK2 AS alleles as the only catalytic subunit of PKA ("tpk1D/D1tpk1-AS tpk2D/D" or "tpk1D/D tpk2D/D1tpk2-AS"). The growth of the strains expressing only WT alleles of TPK1 or TPK2 ("tpk1D/D1TPK1 tpk2D/D" or "tpk1D/D tpk2D/D1TPK2") was not affected by 1NM-PP1 (Fig. 1B).
In C. albicans, the yeast-to-hyphal transition is largely dependent on PKA signaling. We assessed the ability of the PKA AS strains to filament following 3 to 4 hours of exposure to 1NM-PP1 under the filamentation-inducing conditions of Spider medium, GlcNAc, or serum at 37°C. Under all these conditions, a tpk1 tpk2 homozygous deletion mutant was blocked in hyphal morphogenesis (Fig. 1C). Similarly, treatment with 1NM-PP1 blocked filamentation in the tpk1D/D tpk2D/D1tpk2-AS strain; as expected, the tpk1D/D tpk2D/D1TPK2 strain remained filamentous in the presence of analog (Fig. 1C). Unlike TPK2, TPK1 alone is unable to support wild-type levels of filamentation, as strains expressing WT TPK1 as the sole source of the PKA catalytic subunits formed shorter filaments than the WT control (Fig. 1C). Interestingly, cells remained in yeast form in the tpk1D/ D1tpk1-AS tpk2D/D strain even in the absence of 1NM-PP1, suggesting the allele was not fully functional. When we examined a more host-relevant condition that strongly induces filamentation (RPMI with 10% serum in 5% CO 2 at 37°C), the tpk1-AS mutant could support C. albicans hyphal morphogenesis in the absence of 1NM-PP1 to a similar level as the WT TPK1 allele (Fig. 1D). However, filamentation of the tpk1-AS strain was blocked in these culture conditions with 1NM-PP1. Of note, all 1NM-PP1-dependent blocks in filamentation could be observed after 1 h of exposure (data not shown), highlighting the utility of these AS mutants for studying the dynamic process of morphogenesis.
Next, to generate a strain of C. albicans where both TPK1 and TPK2 are replaced with AS alleles, we reintroduced two copies of the AS TPK1 allele into the tpk1D/D tpk2D/D1tpk2-AS strain at the native loci (Fig. 1A, "1 AS &2 AS "). We also generated a strain reconstituted with all four WT TPK alleles (Fig. 1A, "1 WT &2 WT ") as a control. Both strains expressed TPK1 and TPK2 at levels comparable to the wild-type parental strain (Fig. 1E). The strain carrying both AS TPK1 and TPK2 alleles exhibited dose-dependent inhibition of growth and filamentation in the presence of 1NM-PP1 (Fig. 1F and G). Lastly, to provide biochemical evidence for the inhibition of the AS Tpk2 by 1NM-PP1, we purified the Tpk2/Bcy1 holoenzyme from the tpk1D/D tpk2D/D1TPK2 and tpk1D/D tpk2D/D1tpk2-AS strains that were modified to express the C-terminal His 6 -FLAG 3 -tagged Bcy1. The products after one-step Flag-affinity purification were resolved on an SDS-PAGE gel, and silver staining was used to confirm the presence of both components of the holoenzyme (Fig. 1H). Using an in vitro PepTag PKA activity assay (Promega), we confirmed that the Tpk2/Bcy1 holoenzymes phosphorylate a fluorescent peptide substrate in the presence of cAMP. This activity was sensitive to inhibition by 1NM-PP1 in the holoenzyme purified with the AS Tpk2 but not the wild-type version of the kinase (Fig. 1I).
Next, we generated and characterized a Hog1 AS mutant in C. albicans. To do so, we constructed a SAT1-flipper vector (19) containing a wild-type HOG1 allele. Then, indicates prototrophy for the amino acid or resistance to the drug indicated. (B) Treatment with 1NM-PP1 inhibits the growth of AS TPK1 or TPK2 strains. C. albicans strains were grown overnight to saturation in YPD and then diluted to an optical density at 600 nm (OD 600 ) of 0.002 into fresh YPD containing either 2.5 mM 1NM-PP1 or dimethyl sulfoxide (DMSO) vehicle control. Strains were grown at 30°C for 24 h and growth was assessed by OD 600 (see color bar). (C) Treatment with 2.5 mM 1NM-PP1 inhibits filamentation of the AS TPK2 strain. Strains were grown at 37°C in either YPD with 10% serum; Spider medium; or yeast nitrogen base supplemented with 11 mM glucose, 2% casamino acid, and 5 mM GlcNAc (YNBNAG) for 4 h. (D) Treatment with 5 mM 1NM-PP1 inhibits filamentation of the AS TPK1 and TPK2 strains in RPMI with 10% serum and 5% CO 2 at 37°C. Strains were grown for 4 h. (E) TPK1 expression and TPK2 expression are not significantly different in wild-type or AS C. albicans strains. Transcript levels were normalized to ACT1. Data are presented as mean 6 SD of technical triplicates. (F) A strain of C. albicans with both AS TPK1 and TPK2 alleles exhibited analog-specific inhibition of growth. C. albicans strains were grown as described in B. (G) Treatment with 1NM-PP1 inhibits filamentation in a strain of C. albicans with both AS TPK1 and TPK2 alleles. Strains were grown in YNBNAG at 37°C for 4 h. (H) Silver staining of the purified Tpk2/Bcy1 holoenzyme. Strains were grown to log phase in YPD, and then the PKA holoenzyme was purified using a flag-affinity purification as described previously (24). HF represents His6-Flag3. The purified product was run on a 10% SDS-PAGE gel and silver stained. *, impurities. (I) PKA kinase assays were performed using the Peptag kit (Promega). The nonphosphorylated peptide contains one positive charge (11) and migrates toward the cathode. The phosphorylated peptide contains one negative charge (21) and migrates toward the anode. Products were resolved on a 0.8% agarose gel in 50 mM Tris-HCl (pH 8.0). The gel was imaged with an Alexa 568 channel. In all panels, the acronym AS stands for analog sensitive. All experiments were performed in biological duplicate. point mutations conferring N100A or D144A substitutions were introduced into the HOG1 sequence to generate either an AS or kinase dead (KD) mutant, respectively. The vectors were introduced into a hog1D/D (20) mutant at the native locus to generate WT, AS, or KD Hog1 strains ( Fig. 2A).
Complementation of the hog1 homozygous deletion mutant with either the WT or AS allele (in the absence of the analog), but not the KD allele, restored C. albicans growth under osmotic stress induced by 1 M NaCl (Fig. 2B). However, the AS allele of HOG1 in the presence of 1NM-PP1 was unable to support C. albicans growth under this condition (Fig. 2B). Complementation with either the WT or the AS allele also restored the Hog1-mediated transcriptional response to osmotic stress, as measured by the expression of the glycerol 3-phosphatase gene RHR2 (Fig. 2C). Treatment with 1NM-PP1 inhibited the induction of RHR2 in the strain of C. albicans with AS Hog1 (Fig. 2C). Finally, previous work identified Hog1 as a repressor of filamentation (9). 1NM-PP1 treatment induced filamentation of the hog1D/D1hog1-AS strain at 34°C, which is a temperature nonpermissive for the hog1D/D1HOG1 strain to filament (Fig. 2D).
The work presented here describes strains that can be employed to facilitate a deeper understanding of how PKA and Hog1 regulate morphogenesis and stress responses in C. albicans. Our characterization suggests that the AS Tpk2 and AS Hog1 kinases have wildtype-like function and are sensitive to specific inhibition by 1NM-PP1. The AS TPK1 allele, however, is likely hypomorphic, as it supports hyphal morphogenesis only under specific conditions. The partial loss of function is not uncommon for AS kinases (17). Importantly, all strains generated allow for at least one selectable marker to be used for further genetic modification. Future work will continue to develop AS versions of other C. albicans kinases of interest, especially for those proteins explored as potential targets for antifungal development, such as the casein kinases Yck2 and Hrr25 (21,22).