Draft Genome Sequence of a Bovine Enterovirus Isolate Recovered from Sewage in Nigeria

We describe the draft genome of a bovine enterovirus (EV) isolate recovered from sewage in Nigeria. This isolate replicates on both RD and L20B cell lines but is negative for all EV screens in use by the Global Poliovirus Eradication Initiative (GPEI).

E nteroviruses are members of the genus Enterovirus (EV), family Picornaviridae, order Picornavirales. Poliovirus (PV) is the type member of the genus and, courtesy of the Global Poliovirus Eradication Initiative (GPEI), is isolated in about 150 specialized laboratories globally. The laboratories use RD (of human origin) and L20B (engineered mouse cells expressing the poliovirus receptor) cell lines for PV isolation (1,2). All isolates that grow on both cell lines are assumed to be polioviruses and subsequently subjected to molecular identification (3). Here, we describe the genome of an isolate, EV_NGR_2017, that grew on both cell lines but is not poliovirus.
The isolate was recovered from a sewage-contaminated water sample collected in Borno State, Nigeria, in 2017. It was inoculated into (and replicated on) both RD and L20B cell lines but was not poliovirus. The genome of the isolate was extracted using a total RNA extraction kit (Jena Bioscience, Germany) and used for cDNA synthesis as previously described (4). The single-stranded cDNA was shipped to a commercial facility (MR DNA, TX, USA), where library preparation and genome sequencing and assembly were done. Library preparation was done using the TruSeq RNA LT sample preparation kit (Illumina) following the manufacturer's recommendations. Sequencing was done paired end for 300 cycles using the MiSeq system (Illumina), assembly of the 7.8 million reads was done using Newbler (Roche), and annotation of the Enterovirus E (EV-E) genome was done by aligning it (using MEGA5 software [5]) with previously characterized and annotated EV-E genomes in GenBank.
Classically, bovine enteroviruses are cultured in Mardin-Darby bovine kidney (MDBK) cells (6,7). Therefore, investigations of the biological basis of EV_NGR_2017 replication in both human (RD) and mouse (L20B) cell lines and the implications for zoonosis are needed. Kaundal et al. (8) recently described unidentifiable isolates from sewage in India that replicated in both RD and L20B cell lines but were not polioviruses. These isolates might also be bovine enteroviruses.
Data availability. The draft genome assembly and raw reads have been deposited in GenBank and SRA under the accession numbers MH719217 and PRJNA493004, respectively.

ACKNOWLEDGMENT
This research received no specific grant from any funding agency.