Complete Genome Sequence of Bisphenol A-Degrading Bacterium Sphingobium sp. Strain A3, Isolated from Contaminated Soil

This study reports the complete genome sequence of bisphenol A-degrading bacterium Sphingobium sp. strain A3, which was isolated from a contaminated soil sample from the site of a factory fire in South Korea. The genome consists of a 6.53-Mbp chromosome and eight plasmid contigs (532,947 bp), with 6,406 protein-coding sequences and a GC content of 63.82%.

B isphenol A (BPA) is used in plastic bottles and food packaging (1, 2). BPA mimics the structure and function of the hormone estrogen, and it can interfere with normal bodily processes (3). Many studies have suggested that bacteria could provide a promising strategy for xenobiotic cleanup and bioremediation (4,5). BPA-degrading bacteria, such as Achromobacter (6), Pseudomonas (7), Bacillus (8), and Sphingomonas (9), have been isolated. Although the isolation of novel bacteria led to proposed metabolic pathways of BPA degradation using intermediates detected during the degradation process, the genetic mechanisms of BPA degradation are not yet understood (10,11).
The BPA-degrading bacterium Sphingobium sp. strain A3 was isolated from contaminated soil, and the whole genome was sequenced to understand its metabolic capacity and functional potential. Contaminated soil samples were collected at the site of a factory fire (35°18955.10N, 128°45941.00E) in the Gyeongsangnam-do province (South Korea). Enrichment cultures were conducted aerobically at 30°C for 2 weeks, with shaking, using contaminated soil in mineral salt medium (MSM) with 500 ppm BPA as the sole carbon source. The enrichment-cultured samples were diluted in phosphate-buffered saline (PBS) (pH 7.4). The dilutions were then spread on R2A agar plates (BD Difco, USA) and incubated at 30°C for 2 days to obtain a single colony. To determine the BPA-degrading activity using gas chromatography, following the protocols used in previous studies (12), each bacterial colony was seeded in 5 ml R2A medium, and the cells were inoculated into MSM with 300 ppm BPA at 30°C. After 24, 48, 72, and 92 h, the supernatants were obtained and analyzed to determine how much BPA had been removed. We screened the A3 bacterium, which had higher BPA-degrading activity among the single colonies obtained. This strain showed degradation rates of 51.6% 6 11.9%, 57.3% 6 8.1%, 86.9% 6 2.8%, and 96.9% 6 5.4% at 24, 48, 72, and 92 h, respectively.
Genomic DNA was isolated from the A3 strain using Maxwell 16 DNA purification kits (Promega, Madison, WI, USA). The genomic DNA was sequenced at Macrogen, Inc. (South Korea), using a combination of the PacBio RS II single-molecule real-time (SMRT) (13) sequencing platform, with a 20-kb SMRTbell template library, and the Illumina HiSeq X Ten sequencing platform (2 Â 151 bp). For PacBio sequencing, genomic DNA (8 mg) was sheared to approximately 20 kb with a g-TUBE (Covaris) and purified using AMPurePB beads (Beckman Coulter), and the sequencing library was prepared using the SMRTbell template preparation kit v1.0 (PacBio). For Illumina sequencing, genomic DNA (100 ng) was sheared using an LE220 focused ultrasonicator (Covaris) and the sequencing library (350-bp insert size) was generated using a TruSeq Nano DNA library preparation kit. A total of 94,869 PacBio subreads (mean subread length, 6,496 bp; N 50 , 10,298 bp) were generated and used for preassembly and de novo assembly using FALCON-integrate software v2.1.4. A total of 12,165,610 raw Illumina paired-end reads (1.83 Gbp) were generated, and 5,664,022 clean reads (0.85 Gbp), in which $90% of the bases in each read had a Phred score of $30, were used for error correction with Pilon v1.21 for the final genome assembly. UGENE v1.32.0 was used to construct a self-dotplot to check the circularity of contigs. Overlapping ends were trimmed out. The genome was then annotated using NCBI Prokaryotic Genome Annotation Pipeline (PGAP) v4.12 (14). Default parameters were used for all software unless otherwise specified.
Although there is little information available on the enzymes and genes that are involved in the BPA degradation pathway, a few studies suggest that cytochrome P450 monooxygenase (17), laccase (18), lignin peroxidase (19), and manganese peroxidase (20) can degrade BPA. In the A3 genome, 10 P450 cytochromes were found in the chromosome and plasmid, and they showed high levels of similarity to previously reported P450 cytochromes of Sphingobium sp. strain YL23 (10,21). Further indepth biochemical and genomic analyses are needed to better understand BPA degradation pathways.

ACKNOWLEDGMENT
This work was carried out with support from a Nakdonggang National Institute of Biological Resources grant (project NNIBR202002104) funded by the Ministry of Environment, South Korea.