Complete Genome Sequence of Avian Pathogenic Escherichia coli Strain APEC O2-211

Avian pathogenic Escherichia coli (APEC) is the causative agent of colibacillosis, a disease that affects poultry production worldwide and leads to multimillion-dollar losses annually. Here, we report the genome sequence of APEC O2-211, a sequence type 117 (ST117) strain isolated from a diseased chicken.

APEC O2-211 was stored at Ϫ80°C in glycerol prior to sequencing, and the isolate was cultured on MacConkey agar. Next, a single colony was picked and grown in Luria-Bertani broth at 37°C. DNA was extracted using a DNeasy blood and tissue Genomic-tip kit (Qiagen, Hilden, Germany) for Pacific Bioscience (PacBio, Menlo Park, CA) sequencing and a ChargeSwitch genomic DNA (gDNA) mini bacteria kit (Life Technologies, Carlsbad, CA) for Illumina sequencing. DNA yields were quantified using a Qubit fluorometer double-stranded DNA (dsDNA) high-sensitivity (HS) assay kit (Life Technologies). The genomic library for MiSeq sequencing was prepared with a Nextera Flex kit (Illumina, San Diego, CA), and a SMRTbell kit (Pacific Biosciences, Menlo Park, CA) was used to prepare the genomic library for PacBio sequencing with BluePippin (Sage Science, Beverly, MA) size selection to target a library size of 10 kb.
The genome of APEC O2-211 consists of a single chromosome, one large plasmid, and two small plasmids. The chromosome consists of 5,114,241 bp and has a 50.6% GC content. It encodes 89 tRNAs and contains 4,783 coding sequences. The large plasmid, pAPECO2-211A-ColV, is a hybrid IncFIB/IncFIC plasmid. It consists of 197,773 bp with 215 coding sequences and has a 49.1% GC content. The two small plasmids, pAPECO2-211B and pAPECO2-211C, consist of 4,231 and 2,096 bp, respectively. Genomic comparisons of APEC O2-211 with other extraintestinal pathogenic E. coli (ExPEC) are ongoing.
Data availability. The chromosomes and plasmids have been deposited in GenBank under the accession numbers CP006834, CP030791, CP030792, and CP030793. The PacBio and Illumina reads are available in the NCBI Sequence Read Archive (accession number SRP158042).

ACKNOWLEDGMENTS
We thank David Alt and Darrell Bayles (National Animal Disease Center, Ames, IA) for their assistance in sequencing and bioinformatics expertise, respectively. This work was supported by the Dean's Office at Iowa State University's College of Veterinary Medicine and USDA ARS CRIS funds.
Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.