Genome Sequence of Arthrobacter sp. UKPF54-2, a Plant Growth-Promoting Rhizobacterial Strain Isolated from Paddy Soil

Arthrobacter sp. strain UKPF54-2, a plant growth-promoting rhizobacterium having the potential ability to control fungal and bacterial pathogens, was isolated from paddy soil in Kumamoto, Japan. We report here the whole-genome sequence of this strain.

A single colony of Arthrobacter sp. strain UKPF54-2 was cultured in 5-ml nutrient broth culture medium containing 0.3 mM NaNO 3 and 4 mM sodium succinate (pH 7.0) for 2 to 3 days and incubated at 26°C with shaking at 220 rpm. The genomic DNA was extracted with a DNeasy blood and tissue kit (Qiagen, Germany). The template prep kit 1.0 and BluePippin size selection system were used to construct a SMRTbell library with a 20-kb insert size. Whole-genome sequencing was performed on the PacBio RS II DNA sequencing system using C4 chemistry. A total of 1,623,497,927 bases were obtained. The mean subread length was 9,026 bp. The N 50 value of the raw sequences is 11,645 bp. Up to 179,866 reads were obtained by filtration. The Falcon software (v 0.2.1) (12) was used to assemble the PacBio long reads with default parameters, except for daligner-selected overlap detection and error correction of raw reads. One contig was acquired accordingly. The ends of this contig are overlapped to generate a single circular contig for the chromosome. Arthrobacter sp. strain UKPF54-2, with a depth of about 260ϫ, has a circular chromosome size of 3,517,818 bp, with a GϩC content of 68.5%.
A total of 3,110 protein-coding sequences, 50 tRNAs, 15 rRNAs, 3 noncoding RNAs (ncRNAs), and 60 pseudogenes were discovered using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP; revision 4.8) with the best-placed reference protein set (GeneMarks-2ϩ) with default parameters (13,14). Results from BlastKOALA (15) against the species_prokaryotes database with default parameters showed that the annotated functional genes consisted of 155 genes of the transporters, 31 genes of the secretion system, 10 genes of the two-component system, and 30 genes of the bacterial motility proteins. These predicted genes contained candidate genes relevant to plant growth promotion (Table 1). They also contained genes encoding acetolactate synthase (ilvCDN), which is involved in the synthesis of the volatile plant growth promotion signaling molecule acetoin. Furthermore, they contained 5 genes related to antimicrobial resistance.
This report will allow for genome-wide comparative analysis among Arthrobacter species or between this strain and other plant growth-promoting rhizobacterial strains that will provide fundamental support for developing biofertilizer.
Data availability. This whole-genome sequence of Arthrobacter sp. strain UKPF54-2 is available at GenBank under the accession number CP040174. The raw reads have been deposited in the Sequence Read Archive (SRA) under the accession number SRR8929632.

ACKNOWLEDGMENTS
This study was financially supported by the National Natural Science