Draft Genome Sequence of Vibrio jasicida 20LP, an Opportunistic Bacterium Isolated from Fish Larvae

ABSTRACT We present the genome sequence of Vibrio jasicida 20LP, a bacterial strain retrieved from larvae of gilthead seabream (Sparus aurata), a highly valuable, model fish species in land-based aquaculture. Annotation of the V. jasicida 20LP genome reveals multiple genomic features potentially underpinning opportunistic associations with diverse marine animals.

V ibrio jasicida is a relatively recently described bacterial species that belongs to the Vibrio harveyi clade and can be found in association with diverse marine animals, such as lobsters, gastropods, and fish (1). Vibrio jasicida (formerly classified as V. harveyi) has been proposed as the causative agent of vibriosis, leading to mortality rates of .75% for phyllosoma larvae of the packhorse rock lobster Jasus verreauxi (2), which raises concerns about the pathogenic potential of this species across a broad range of marine animals in both natural and built environments. To increase our understanding of the species' aptitude to colonize, persist, and exploit animal hosts, here we report the genome sequence of V. jasicida strain 20LP.
V. jasicida 20LP was isolated after cultivation of microbial cell suspensions derived from gilthead seabream larvae on thiosulfate-citrate-bile salts-sucrose (TCBS) agar (Oxoid, USA) for 7 days at 22°C (3) and was classified by 16S rRNA gene sequencing using the sequence match tool of the RDP database (http://rdp.cme.msu.edu), as described previously (4). Prior to genome sequencing, DNA was obtained with the Wizard genomic DNA purification kit (Promega, USA) from a fresh culture prepared in marine broth for 2 days at 19°C (5). The Illumina Nextera XT DNA library preparation kit (insert size, ;450 bp) was employed for library construction, and paired-end sequence reads (125 cycles) were generated on an Illumina HiSeq 2500 platform at BaseClear (The Netherlands). For all bioinformatic analyses, default parameters were used unless specified otherwise. FASTQ sequence files were created with the Illumina Casava pipeline v1.8.3. Thereafter, reads containing adapters and low-quality reads were removed using BBtools v38.86 (http://bbtools.jgi.doe.gov). The sequencing output was 467.4 Mb, comprising 3,709,520 reads of 126 bp. Genome assembly was performed with the de novo assembly option within the CLC Genomics Workbench v7.0.4, and the optimal k-mer size was automatically determined using KmerGenie v1.6213 (6). Table 1 displays the general features of the V. jasicida 20LP genome, which possesses 97.8% average nucleotide identity (ANI) with respect to the genome of the type strain V. jasicida LMG 25398 (GenBank accession number GCF_000400365.1), as assessed on the IMG/M platform v6.0 (7).
Data availability. The genome sequence was deposited in the European Nucleotide Archive (ENA) under BioProject accession number PRJEB9149, BioSample accession number SAMEA7110814, GenBank assembly accession number GCF_903995475.1, and SRA accession number ERR6053175. The annotation reported in this study is available on the RAST platform for guest users under job number 877706, identification number 6666666.587973.

ACKNOWLEDGMENTS
This study was financed by the Portuguese Foundation for Science and Technology (FCT), I.P., through research grants PTDC/MAR/112792/2009 and PTDC/BIA-MIC/31996/ 2017 and by the European Regional Development Fund (ERDF) (project 031996, operational code ALG-01-0145-FEDER-031966) through the regional operational programs of Lisbon and Algarve, Portugal. This study was also financed by the FCT in the scope of projects UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute for Bioengineering and Biosciences and project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy.
We thank Walter Pirovano from Baseclear for providing details on genome sequencing methods.