Draft Genome Sequences of Mycoplasma mycoides subsp. mycoides Strains APF9 and AP108, Isolated in Nigeria in 2014 to 2016

Mycoplasma mycoides subsp. mycoides is the etiological agent of contagious bovine pleuropneumonia (CBPP). While several findings on CBPP prevalence in Nigeria were documented, no data were reported about the genomic characterization of Nigerian M. mycoides subsp. mycoides strains. Here, we present the draft genome sequences of two novel M. mycoides subsp. mycoides strains isolated in Nigeria.

M ycoplasma mycoides subsp. mycoides is the causative agent of contagious bovine pleuropneumonia (CBPP), a severe contagious respiratory disease affecting cattle, which is included among the Office International des Epizooties (OIE) diseases (1).
Most countries have eradicated CBPP (http://www.oie.int/en/animal-health-in-the -world/official-disease-status/cbpp), but the disease still remains endemic in sub-Saharan Africa, including Nigeria, where it causes, directly or indirectly, economic losses of about $2 billion annually (2).
Epidemiological studies were performed in the northeastern region of Nigeria (3, 4), but no extensive work was carried out on the genomic characterization of Nigerian M. mycoides subsp. mycoides isolates, which would be of great importance in order to clarify the relationship among strains circulating in different regions of the country, providing support to CBPP outbreak investigations and disease control.
The draft genome sequences of two novel M. mycoides subsp. mycoides strains, named APF9 and AP108, are presented here. The first strain, APF9, was isolated in 2014 from pleural fluid of a cow showing pathological lesions referring to CBPP at slaughter in the Yola Modern Abattoir (Adamawa State, Nigeria) (5). The AP108 strain was isolated in 2016 from pleural fluid of a regularly slaughtered cow in the Yola Modern Abattoir.
The isolates were grown at 37°C under 5% CO 2 in pleuropneumonia-like organism (PPLO) broth and agar (Difco) supplemented as previously described (6). The genomic DNA was extracted using a Maxwell 16-cell DNA purification kit (Promega), and a PCR-based test (7) was used for the identification of M. mycoides subsp. mycoides. Library preparation was carried out using a Nextera XT library prep kit (Illumina, Inc.), according to the manufacturer's manual. The libraries were loaded onto an Illumina 300-cycle NextSeq 500/550 mid output reagent cartridge 2 kit and sequenced on an Illumina NextSeq 500 platform, producing 150-bp paired-end reads.
Data availability. The genome sequences were deposited in GenBank under the following accession numbers: VCPG00000000 (strain APF9) and VCPH00000000 (strain AP108). The Illumina raw reads were deposited in the NCBI Sequence Read Archive (SRA) under the accession numbers SAMN11775870 (strain APF9) and SAMN11775871 (strain AP108).

ACKNOWLEDGMENTS
This work was sponsored by the Italian Ministry of Health, grant code IZS AM 01/14 RC.
The mention of trade names or commercial products in this article is only for the purpose of providing specific information and does not imply recommendation or endorsement by the OIE Reference Laboratory for Contagious Bovine Pleuropneumonia in Teramo, Italy.