Whole-Genome Sequence of Bacillus subtilis WS1A, a Promising Fish Probiotic Strain Isolated from Marine Sponge of the Bay of Bengal

This study reports the draft genome sequence of a promising fish probiotic, Bacillus subtilis strain WS1A, that possesses antimicrobial activity against Aeromonas veronii and suppressed motile Aeromonas septicemia in Labeo rohita. The de novo assembly resulted in an estimated chromosome size of 4,148,460 bp, with 4,288 open reading frames.

T he Bacillus subtilis strain WS1A was isolated from a marine sponge from the Saint Martin's Island area of the Bay of Bengal, Bangladesh. WS1A was cultured on Zobell agar plates (1,2). It can grow in both marine water and freshwater media. It demonstrated in vitro antimicrobial activity against Aeromonas veronii, prevented motile Aeromonas septicemia in an Indian major carp species (Labeo rohita) (3,4), and is considered a promising probiotic candidate. Prior permission was obtained from the Institute of Biotechnology and Genetic Engineering ethical review committee for the animal experiments (approval number IBGE-ERC-005).
The annotated chromosome length, GC content, and N 50 value of the assembled genome were 4,148,460 bp (151 contigs), 43.6%, and 199,148 bp, respectively. The largest and smallest contigs were 433,806 bp and 257 bp, respectively. The open reading frames of the genome were predicted and annotated using the Rapid Annotations using Subsystems Technology (RAST) server (classic RAST FIGfams v70) (18), which showed 333 subsystems and 96 RNA genes.
Genome analyses identified several orthologs of intrinsic genes of a potential probiotic bacterium, such as those encoding proteins involved in the biosynthesis of riboflavin, vitamin B 6 , and amino acids (ilvD) and in carbon utilization (pta). The genome also codes for antimicrobial peptides such as bacillaene, subtilin, bacillibactin, surfactin, fengycin, bacilysin, and subtilosin A (antiSMASH v5.1.2) (19). No genes coding for putative virulence factors, no plasmids, and no antibiotic resistance genes were identified in the genome using VirulenceFinder v2.0 (16), Plas-midFinder v2.0 (15), and ResFinder v4.0 (14), respectively, with default parameters. The genome sequence information will help to exploit the probiotic potential of this strain.
Data availability. The whole-genome shotgun project for B. subtilis strain WS1A has been deposited at GenBank under the assembly accession number JABFHE000000000.1. Raw reads and raw sequencing data are available under the accession number SRR11868367, BioProject accession number PRJNA630208, and BioSample accession number SAMN14828537.

ACKNOWLEDGMENTS
We acknowledge the Bangabandhu Sheikh Mujibur Rahman Agricultural University Research Management Wing and the Ministry of Science and Technology for providing research grants for the research projects "Development of native probiotics, herbal extracts and antimicrobial agents for sustainable management of major fish diseases of Bangladesh" and "Identification of antibiotics and their associated genes in sponge-associated bacteria of the Saint Martin's Island inhibiting fish and shrimp pathogens," respectively.
There are no conflicts of interest regarding this paper.