Metagenome-assembled genomes from particle-associated microbial communities in the mesopelagic zone of the Pacific Ocean

ABSTRACT We report 10 particle-associated metagenome-assembled genomes (MAGs) from the mesopelagic zone of Pacific Ocean seawaters. MAGs comprise members of Flavobacteria Halomonas, Blastomonas, Brevundimonas, Alteromonas, Shingomonas, Sphingopyxis, Tabrizicola, Proteobacteria, and Gammaproteobacteria. Functional annotation suggests that these bacteria are involved in central particulate organic carbon conversion, nitrogen cycling, and phosphorus cycling.

T he metagenome-assembled genomes (MAGs) derived from a water sample were employed for the investigation of the particle-associated microbial community of a mesopelagic zone in the Pacific Ocean.
The water sample was collected in May 2016 at the SONNE research vessel cruise SO248 (BacGeoPac) in the Pacific Ocean (28 °N; 177.33 °E) at a depth of in 300 m.Approximately 10 L were filtered through a 20-µM nylon net (Millipore, Billerica, MA, USA) and through a 2.7-µM glass fiber filter (Whatman GF/D; GE Healthcare, Little Chalfont, UK) to investigate the particle-associated microbial community.The filter was kept at −80°C until further usage.The total community DNA was extracted according to Hollensteiner et al. (1).Illumina pair-end sequencing libraries were prepared and indexed using the Nextera DNA Flex library preparation kit and the Nextera DNA CD index kit as recommended by the manufacturer (Illumina, San Diego, CA, USA).Sequencing was performed with an Illumina NovaSeq 6000 system, the NovaSeq 6000 SP Reagent Kit v1.5 (paired-end mode with 2 × 250 bp cycles), and the NovaSeq XP 2-Lane Kit (v1.5 chemistry).
in study design, data collection and analysis, the decision to publish, or the preparation of the manuscript.

TABLE 1
Features of MAGs from the Pacific Ocean a

Tk genus classification BioSample Compl./ cont. Size (bp)
a High-quality MAGs are displayed in bold.