Draft Genome Sequence of Streptomyces sp. Strain ventii, Isolated from a Microbial Mat near Hydrothermal Vents within the Axial Seamount in the Pacific Ocean, and Resequencing of the Type Strains Streptomyces lonarensis NCL 716 and Streptomyces bohaiensis 11A07

The draft genome of Streptomyces sp. strain ventii, an environmental isolate recovered from deep-sea hydrothermal vents in the Pacific Ocean, is presented along with the resequenced draft genomes of the type strains Streptomyces bohaiensis 11A07 and Streptomyces lonarensis NCL 716.

M embers of the genus Streptomyces are Gram-positive, spore-forming, filamentous bacteria that often synthesize desirable antimicrobials, cytotoxins, and other lead compounds (1)(2)(3)(4). The type strains Streptomyces bohaiensis 11A07 and Streptomyces lonarensis NCL 716 produce antimicrobials and an ␣-amylase, respectively (5-7). Streptomyces sp. strain ventii was isolated from the Juan de la Fuca Ridge in the Northeast Pacific Ocean. The draft genome of Streptomyces sp. ventii is presented, along with the resequenced draft genomes of S. bohaiensis 11A07 and S. lonarensis NCL 716.
Deep-sea samples were collected during the 2011 New Millennium Observatory expedition, run through the National Oceanic and Atmospheric Administration (NOAA) Vents Program at Oregon State University and the NOAA Pacific Marine Environmental Laboratory. A microbial mat near hydrothermal vents on the Axial Seamount (46.06°N, 130°W) at a depth of 2,190 m was collected with a custom syringe-based sampler on the remotely operated vehicle (ROV) Jason II (aboard the research vessel [R/V] Thompson). The sample was diluted 1:1,000 in sterile Instant Ocean, spread onto 1/10 Zobell marine agar 2216 with sterile swabs, and incubated at 28°C for 2 weeks. Strain maintenance was performed on International Streptomyces Project 2 (ISP2) medium supplemented with 0.1 M sodium phosphate buffer to a pH of 8.0 (buffered ISP2) at 28°C (8). Strains 11A07 (DSM 42125) and NCL 716 (DSM 42084) were obtained from the Leibniz Institute DSMZ and cultured on buffered ISP2 medium at 28°C. Streptomyces sp. ventii was confirmed as a member of the Streptomyces genus through 16S rRNA gene sequencing and BLAST analysis (9,10). Following a 4-day incubation at 28°C in buffered ISP2 broth shaken at 120 rpm, DNA was isolated by phenol-chloroform extraction (11). The raw reads were obtained from the Microbial Genome Sequencing Center, LLC (Pittsburgh, PA), using 151-bp paired-end read libraries prepared with the Illumina Nextera kit (12). Libraries were run on the Illumina NextSeq 550 platform yielding 9,643,560, 12,101,213, and 14,343,200 pairs of raw reads for  The published assemblies were filtered with BBMap to remove scaffolds and contigs smaller than 1,000 bp, as was done for genome sequences presented in this work, and analyzed with the same software.
c Data for the genome assemblies with all contigs/scaffolds Ͻ1,000 bp removed.
Loughran et al.
Data availability. The whole-genome shotgun projects, BioSample material, and raw reads have been deposited in DDBJ/ENA/GenBank under the accession numbers listed in Table 1.