Draft Genome Sequences of Three Pasteurella multocida Strains Isolated from Domestic Animals in Kazakhstan

We report here the draft genome sequences of three strains of Pasteurella multocida isolated in Kazakhstan from domestic animals that died due to hemorrhagic septicemia.

P asteurella multocida is a Gram-negative bacterium causing pasteurellosis, which is most dangerous in the form of hemorrhagic septicemia (HS). In Kazakhstan, pasteurellosis is an endemic infection, with sporadic cases among domestic animals and pandemic outbreaks among saiga populations. The case fatality rate due to HS can reach 90% of the animal population. An example of this is the outbreaks between 2008 and 2015, which led to a catastrophic decline in saiga populations, putting them on the brink of extinction (1). Information on the genetic diversity of P. multocida circulating in Kazakhstan among wild and particularly domestic animals is limited (2,3). The goal of this work was to improve our knowledge of P. multocida strains circulating in domestic animals in Kazakhstan.
Three strains of P. multocida were deposited in the collection of the National Reference Veterinary Center. The strains P-mult-5-KZ, P-mult-15-KZ, and P-mult-10-KZ were isolated in 2006, 2015, and 2013, in the Kostanay, Almaty, and East Kazakhstan regions of Kazakhstan, from pathological material from two horses and cattle, respectively. Isolates were recovered by seeding a 10% suspension of the liver and spleen onto nutrient agar (HiMedia, India) for 24 h at 37°C. Single colonies were subcultured in a nutrient broth (HiMedia) for 24 h at 37°C. The culture of the P-mult-5-KZ strain was stored in a lyophilized state, followed by a repeat of the procedures described above. DNA was isolated from the accumulated cultures using the QIAamp DNA minikit (Qiagen, USA), with subsequent 16S rRNA gene identification (4), and then the DNA was stored at Ϫ70°C. These strains are no longer available from the National Reference Veterinary Center.
The assembly characteristics and genotypes are presented in Table 1. Genotyping of the capsular, lipopolysaccharide, and multihost MLST loci showed that all three strains belong to the B:L2:multihost ST64 genotype. The genotyping results are similar to the previously described data in the study by Orynbayev et al. (2). Therefore, it can be judged that the B:L2:multihost ST64 is a major characteristic genotype of the P. multocida strains circulating in Kazakhstan. The obtained sequence data will be helpful for revealing the genetic differences responsible for the virulence and pathogenicity of the hemorrhagic septicemia-associated strains of P. multocida.
Data availability. This whole-genome shotgun project has been deposited in DDBJ/ ENA/GenBank under accession numbers JAAONW000000000, JAAILE000000000, and JAA-ONX000000000. The raw data from BioProject PRJNA556768 were submitted to the NCBI SRA under accession numbers SRR11293610, SRR9841486, and SRR11293647.

ACKNOWLEDGMENT
This work was funded by grant number AP05133053 from the Ministry of Education and Science of the Republic of Kazakhstan.