Genome-sequenced bacterial collection from sorghum aerial root mucilage

ABSTRACT A collection of 47 bacteria isolated from the mucilage of aerial roots of energy sorghum is available at the Great Lakes Bioenergy Research Center, Michigan State University, Michigan, USA. We enriched bacteria with putative plant-beneficial phenotypes and included information on phenotypic diversity, taxonomy, and whole genome sequences.

them to corresponding 16S rRNA gene amplicon dynamics in a field microbiome study that was executed at the same field site at Kellogg Biological Station (3).Individual isolates or the full cryopreserved collection is available.Cultures can be regrown using standard microbiological procedures to transfer a sterile inoculation loop of freezer stock onto the original isolation medium and incubation time (Table 1).

7 A 7 S
na s sp .SO RG H_ AS 21 1 Pse udo mon as sp.SO RG H_A S 199 Pseud omona s psychr otolera ns SORG H_AS 201 Pseudomonas aeruginosa PAO1 Pseudo momas fluoresc ens SORGH _AS 191 Pan toea ana nati s SOR GH_ AS 197 Pa nto ea an an ati s SO RG H_ AS 21 3 Pa nt oe a an th op hi la SO RG H_ AS 79 tla nt ib ac te r sp .S O R G H _A S 30 4 E n te ro b a ct e r sp .S O R G H _ A S 2 8 te n o tr o p h o m o n a s s p .S O R G H _ A S 3

TABLE 1
Taxonomy, colony phenotype, and genome characteristics of 49 bacterial isolates from sorghum aerial root mucilage, as described in this study a

Isolate ID Bacterial species Initial isolation media Temperature, oxygen availability Colony phenotype Sequencing platform SRA accession number GenBank accession Genome length (bp) No. of contigs Coverage GC% Genome completen ess (%)
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TABLE 1
Taxonomy, colony phenotype, and genome characteristics of 49 bacterial isolates from sorghum aerial root mucilage, as described in this study a

TABLE 1
Taxonomy, colony phenotype, and genome characteristics of 49 bacterial isolates from sorghum aerial root mucilage, as described in this study a SRA is the sequence read archive of the U.S. National Institutes of Health.