Draft Genome Sequence of the Bacterium Paraburkholderia aromaticivorans AR20-38, a Gram-Negative, Cold-Adapted Degrader of Aromatic Compounds

Here, we report the draft genome sequence of Paraburkholderia aromaticivorans strain AR20-38, a cold-adapted Gram-negative bacterium. It was isolated from Alpine forest soil and can degrade a range of aromatic compounds.

P araburkholderia is a genus of Proteobacteria, class Betaproteobacteria. Members of this genus have been isolated from diverse ecological niches, including pristine and contaminated soil, sediments, rocks, and plants (1,2).
The strain was grown from a single colony on R2A agar and was further inoculated in nutrient broth incubated at 10°C until the stationary growth phase. After lyophilization, genomic DNA was extracted using lysozyme, SDS, and phenol-chloroformisoamyl alcohol. DNA quality and quantity were determined using a Qubit 2.0 fluorometer (Thermo Fisher Scientific) and agarose gel electrophoreses. DNA was used for Oxford Nanopore and Illumina sequencing.
The one-dimensional (1D) ligation sequencing kit (SQK-LSK109 kit; Oxford Nanopore) was used with additional reagents from New England Biolabs (NEBNext FFPE repair mix, NEBNext end repair/dA-tailing module, and NEBNext quick ligation module) following the manufacturer's recommendations. No size selection or shearing was applied.
For Illumina sequencing, 1 g DNA was used with the NEBNext Ultra DNA library prep kit (New England Biolabs) following the manufacturer's recommendations. The Nanopore library was sequenced on the PromethION instrument (PromethION flow cells, FLO-PRO002; Oxford Nanopore), and the Illumina library was sequenced on the Illumina NovaSeq PE150 instrument at the Beijing Novogene Bioinformatics Technology Co. Ltd.
Data availability. The assembled genome and sequencing reads have been deposited in GenBank under the BioProject number PRJNA624061 and the accession numbers CP051514, CP051515, CP051516, and CP051517 and in the NCBI Sequence Read Archive under the numbers SRX8492130 and SRX8492131.