Metagenome-assembled genome sequences of two bacterial species from polyvinyl alcohol-degrading co-colonies

ABSTRACT We present the metagenome-assembled genome sequences of two polyvinyl alcohol-degrading co-colony-derived bacterial species relative to Rhodanobacter sp. DHB23 and Priestia megaterium ATCC 14581. We estimated the genomes of these species to be 3,476,996- and 5,169,587-bp long (for Rhodanobacter sp. DHB23 and Priestia megaterium ATCC 14581, respectively).

V arious industries extensively use polyvinyl alcohol (PVA) although it pollutes the environment (1).Even with activated sludges (AS) in municipal wastewater treatment plants (MWWTP), decomposing PVA is difficult (2,3).Moreover, PVA-degrad ing microorganism genomes and ecology in environments like AS are poorly under stood.Here, we describe MWWTP AS-derived PVA-degrading species and their genome sequences.
We obtained an AS sample from the MWWTP of Toyohashi City, Japan.We inoculated the AS washed with an inorganic medium (4) into 50 mL of the inorganic medium, amended with 0.02% PVA [SOLUBLON GA (Mn: 44000), Aicello CO LTD, Japan] in a 500-mL shaking flask and incubated at 60 rpm and 30°C.We measured the PVA concentration within the culture supernatant using the Deng method (5) and spread the culture dilutions onto a 0.02% PVA-containing agar plate, cultivated at 30°C to screen PVA-degrading colonies.We repeatedly streaked a colony, named ACL01, forming a transparent circle on an iodine-stained PVA agar plate, for purification.
Two bacterial genomes were retrieved from ACL01 colonies on the PVA plate which were morphologically uniform in shape.MAG sequences 1 and 2 were related to Rhodanobacter sp.DHB23 and Priestia megaterium ATCC 14581 (ANI values: 89.04% and 96.24%), respectively.The origins of sequences 3 and 4 remain clear.We found a homolog of PVA dehydrogenase in sequence 1 (Genbank: BFI96117.1)and oxidized PVA hydrolase homologs in either sequence 1 (Genbank: BFI95560.1)or sequence 2 (Genbank: BFI99320.1 and BFJ00521.1).The two species form co-colonies on the PVA agar and may collaborate to decompose PVA using those enzymes.The MAG sequences could advance studying the associations between the co-colony-forming species and provide significant insights into PVA-degrading microbes in various environments.

AUTHOR AFFILIATIONS
1 Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan 2 Basic Technology Research Division R&D Department, AICELLO CORPORATION, Aichi, Japan

TABLE 1
Sequencing metrics and genome statistics a No CRISPR arrays were detected in all sequences.b Not applicable.c Average nucleotide identities.