Genome Sequences of Three African Swine Fever Viruses of Genotypes IV and XX from Zaire and South Africa, Isolated from a Domestic Pig (Sus scrofa domesticus), a Warthog (Phacochoerus africanus), and a European Wild Boar (Sus scrofa)

We report here the genome sequences of three African swine fever virus isolates obtained from a domestic pig (Zaire [Zaire]), a warthog (RSA/W1/1999 [South Africa]), and a European wild boar (RSA/2/2004 [South Africa]) belonging to genotypes IV, XX, and XX, respectively. This report increases the number of genotype XX, wild boar, and warthog reference sequences available.

A frican swine fever virus (ASFV) is the only species belonging to the Asfivirus genus of the Asfarviridae family and is comprised of a complex, linear, double-stranded DNA genome characterized by identical, terminal, inverted repeats and hairpin loops at both ends. The length of the genome is between 170 and 190 kbp and encodes 150 to 167 open reading frames (ORFs) (1)(2)(3)(4). ASFV causes African swine fever, a hemorrhagic disease affecting domestic pigs and wild boars that results in mortality rates approaching 100%. Currently, there is only one genome sequence (GenBank accession number AY261366) of an ASFV isolate from a warthog and one genome sequence (GenBank accession number AY261363) of a genotype XX isolate. The lack of described and fully sequenced isolates from different geographical regions and hosts, including warthogs, ticks, wild boars, and domestic pigs, is one of the major gaps identified in the ASFV field (5-7), and thus we report on three novel ASFV isolated from different hosts.
The ASF viruses were originally isolated on primary murine bone marrow macrophages (PBMC) from tissue samples collected from infected suids. The first virus, Zaire, was isolated from a domestic pig in Zaire in 1977 and subsequently passaged on rhesus monkey kidney epithelial cells (LLC-MK2) and African green monkey cells (Vero). RSA/W1/1999 and RSA/ used to confirm the ASFV nucleic acid presence and genotype, respectively, as described previously (8). CLC Genomics Workbench v9.0.1 was used to generate an unrooted maximum-likelihood tree (HKY model with rate and topology variation and 1,000 bootstrap replicates) based on the multiple sequence alignment (MSA) of partial (399-nucleotide [nt]) p72 sequences from 43 ASFV isolates. Genome sequencing was performed on an Illumina MiSeq instrument using the Nextera XT DNA sample preparation kit and the v3 reagent kit for 2 ϫ 300-bp paired-end reads (Illumina, USA). The tagmented DNA was mixed with index adapter following the manufacturer's instructions; then, 15 l of the Nextera PCR master mix (NPM) was added to each well, the mix was pipetted 10 times, and the plate was sealed. The plate was then centrifuged at 280 ϫ g at 20°C for 1 min and placed on the thermal cycler, and the NXT PCR program was run. De novo assembly of all reads was performed using CLC to generate genomic contigs. Smaller nonspecific contigs were identified as belonging to the host genome. GATU (9) was used to annotate the genomes, using BA71V (10) as the reference; CLC was used to search for ORFs in regions that were missed by GATU, and a BLASTx search was performed against the NCBI nonredundant (nr) protein database to confirm the ORFs. All ORFs intact at their 5= end but comprising less than 80% of ortholog length were annotated as truncated genes, while those ORFs disrupted at their 5= end were annotated as fragmented genes (11). The Zaire, RSA/W1/1999, and RSA/2/2004 isolates showed similar genome statistics in terms of genome length, number of ORFs, and GϩC content ( Table 1). The genomic termini were not sequenced.
Multiple sequence alignment and phylogenetic analysis of the C terminus of the p72 gene showed that Zaire belongs to genotype XX, RSA/W1/1999 to genotype IV, and RSA/2/2004 to genotype XX (Fig. 1), with Zaire showing the closest BLASTn (NCBI nr database) identity to Benin/97/1 from Benin (genotype I; GenBank accession number EF121428), RSA/W1/1999 to Lillie from South Africa (genotype XX; DQ250109), and RSA/2/2004 to wart from Namibia (genotype IV; AY578706). A BLASTn search of the full-genome sequences against the NCBI nr database with default parameters showed that the genome sequences most similar to Zaire, RSA/W1/1999, and RSA/2/2004 were Pretoriuskop/96/4 (AY261363; genotype XX; 97% identity and 99% coverage) and Warthog (AY261366; genotype IV; 98% identity and 99% coverage), respectively. These data add the second warthog genome sequence and two more genotype XX isolates from a domestic pig and a wild boar to the collection of sequences available for comparative genomic analysis.

ACKNOWLEDGMENTS
This work is based on research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation (NRF), South Africa (64815), and the Defense Threat Reduction Agency of the United States Department of Defense (HDTRA1-16-1-0050).
Any opinion, finding and conclusion, or recommendation expressed in this material is that of the authors, and the NRF does not accept any liability in this regard. The funders had no role in the study design, the data collection and interpretation, or the decision to submit the work for publication. Further, the content of the information does not necessarily reflect the position or policy of the federal government, and no official endorsement should be inferred.