Complete Genome Sequence of Escherichia coli GW-AmxH19, Isolated from Hospital Wastewater in Greifswald, Germany

The Gram-negative and rod-shaped Escherichia coli strain GW-AmxH19 was isolated from university hospital wastewater in Greifswald, Germany. The genome consists of two replicons, including one circular chromosome (5.04 Mb) and a circular plasmid (126.96 kb). The genome harbors 4,694 protein-coding genes, comprising multidrug resistance and a potential association with urogenital tract infections.

T he strain Escherichia coli GW-AmxH19 is a Gram-negative, mesophilic, and rodshaped bacterium. It was isolated from 10 ml of saline (0.9% NaCl)-diluted university hospital wastewater in Greifswald, Germany. Members of the genus Escherichia are well distributed in wastewater systems all over the world (1,2). One feature of Escherichia spp. is their ability to withstand different antibiotics (3).
The complete genome consists of one circular chromosome (5,037,322 bp) with an overall GϩC content of 50.52% and a circular plasmid (126,964 bp) with a GϩC content of 50.49%. The genome annotation was performed with Prokaryotic Genome Annotation Pipeline (PGAP) v4.11 (9). The predicted 4,947 genes included 85 tRNA genes, 22 complete rRNA genes, 6 noncoding RNA (ncRNA) genes, and 4,694 protein-coding genes.
Data availability. The whole-genome shotgun project of Escherichia coli GW-AmxH19 has been deposited at GenBank under the accession numbers CP048647 (chromosome) and CP048648 (plasmid), with BioProject accession number PRJNA524094 and SRA accession numbers SRR11014407 (Oxford Nanopore reads) and SRR11014408 (Illumina reads).

ACKNOWLEDGMENTS
We thank Melanie Heinemann and Sarah Teresa Schüßler for technical assistance. Additionally, we thank Arne Krüger and Max Woggon for assistance in sample acquisition.
This study was partly supported by the Bundesministerium für Bildung und Forschung (BMBF) as part of the ANTIRES project (BMBF grant 03ZZ0815A/B). The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.