Coding-Complete Genome Sequence of an African Swine Fever Virus Strain Liv13/33 Isolate from Experimental Transmission between Pigs and Ornithodoros moubata Ticks.

Here, we report the coding-complete genome sequence of African swine fever (ASF) virus strain Liv13/33, isolated from experimentally infected pigs and Ornithodoros moubata ticks. The 11 sequences that we obtained harbored no notable differences to each other, and all of them were closely related to the genome sequence of the Mkuzi 1979 strain of genotype I.

A frican swine fever is a contagious and highly lethal disease of pigs and wild suids caused by the African swine fever virus (ASFV) (Asfarviridae, Asfivirus) and may involve soft ticks of the Ornithodoros genus as vectors and reservoirs of the virus (1).
The strain Liv13/33 was initially isolated in 1983 from a tick of the Ornithodoros moubata group in Livingstone (Zambia, Africa) (2,3). This strain was previously sequenced on two genes (B646L/P72 and E182L/P54) and identified as belonging to the genotype I (4,5). The coding-complete genome sequence of Liv13/33 presented in this report was obtained from samples of a study that experimentally tested the vector competence of O. moubata sensu stricto ticks on pigs as previously described (6). Three 7-week-old specific-pathogen-free (SPF) Large White pigs were inoculated by the intramuscular route with a 10 4 of the 50% hemadsorbing dose (HAD 50 ) of the ASFV Liv13/33 strain. Two hundred and sixty ASFV-free ticks were engorged on infected pigs on the first day of hyperthermia, when pig viremia ranged from 10 7.8 to 10 8.1 HAD 50 /ml. Five engorged ticks were frozen 3 months postinfection for sequencing analysis, and the others were fed on three new SPF pigs to assess their ability to transmit ASFV to naive pigs. These pigs displayed hyperthermia 2 days after being bitten by infected ticks.
Animal experiments performed at the air-filtered biosafety level 3 animal facilities at ANSES-Ploufragan were authorized by the French Ministry for Research (project no. 2017062615498464) and approved by the national ethics committee (authorization no. 11/07/17-3).
DNA was extracted using the High Pure PCR template preparation kit (Roche Life Science) from 400 l of heparin blood samples collected from the six pigs during the viremia peak and from supernatants of crushed ticks prepared by filtration at 0.45 m.  (7); the obtained sequence was thus probably shorter than that in reality. A comparison of the 11 genomes obtained showed fewer than 7 nucleotide differences that were mainly in ITRs and up to 34 gaps located in mononucleotide repeats A or T ( Table 1). The coding-complete genome sequence of Liv13/33 with the best coverage (72.09ϫ) was isolated from tick OmLF2 (Table 1). This sequence of 188,277 bp (GϩC content of 38.4%) harbored 228 open reading frames (ORFs) annotated with the help of Prokka (Galaxy version 1.13) based on the annotations of genomes available on the African Swine Fever Virus Database (http://asfvdb.popgenetics.net/), which proposed the most complete and homogeneous revised annotation (8).
Data availability. The coding-complete genome sequence of isolate OmLF2 has been deposited in GenBank under the accession no. MN913970. Raw data from the 11 isolates for this project can be found in the GenBank SRA under accession no. PRJNA587575.

ACKNOWLEDGMENTS
We are grateful to Vectopole Sud for funding the insectary where O. moubata was raised and to the Direction Générale de l'Alimentation for the financial support to CIRAD. We are thankful to CIRAD and ANSES for funding the Ph.D. grant of R. Pereira de Oliveira. We are also thankful to the NSF-NIH-EEID ASF project (grant no. 2019-67015-28981) for their financial support to the publication of this research. This article is based upon work from COST Action ASF-STOP, supported by COST (European Cooperation in Science and Technology; www.cost.eu).
We thank the technical staff of CIRAD laboratory for raising the ASFV-free ticks as well as staff of the SPPAE Unit for animal care and sampling at ANSES-Ploufragan.