Homologous acetone carboxylases select Fe(II) or Mn(II) as the catalytic cofactor

ABSTRACT Acetone carboxylases (ACs) catalyze the metal- and ATP-dependent conversion of acetone and bicarbonate to form acetoacetate. Interestingly, two homologous ACs that have been biochemically characterized have been reported to have different metal complements, implicating different metal dependencies in catalysis. ACs from proteobacteria Xanthobacter autotrophicus and Aromatoleum aromaticum share 68% sequence identity but have been proposed to have different catalytic metals. In this work, the two ACs were expressed under the same conditions in Escherichia coli and were subjected to parallel chelation and reconstitution experiments with Mn(II) or Fe(II). Electron paramagnetic and Mössbauer spectroscopies identified signatures, respectively, of Mn(II) or Fe(II) bound at the active site. These experiments showed that the respective ACs, without the assistance of chaperones, second metal sites, or post-translational modifications facilitate correct metal incorporation, and despite the expected thermodynamic preference for Fe(II), each preferred a distinct metal. Catalysis was likewise associated uniquely with the cognate metal, though either could potentially serve the proposed Lewis acidic role. Subtle differences in the protein structure are implicated in serving as a selectivity filter for Mn(II) or Fe(II). IMPORTANCE The Irving-Williams series refers to the predicted stabilities of transition metal complexes where the observed general stability for divalent first-row transition metal complexes increase across the row. Acetone carboxylases (ACs) use a coordinated divalent metal at their active site in the catalytic conversion of bicarbonate and acetone to form acetoacetate. Highly homologous ACs discriminate among different divalent metals at their active sites such that variations of the enzyme prefer Mn(II) over Fe(II), defying Irving-Williams-predicted behavior. Defining the determinants that promote metal discrimination within the first-row transition metals is of broad fundamental importance in understanding metal-mediated catalysis and metal catalyst design.

Mn(II), which associates weakly with incoming ligands, stably into an enzymatic binding site.
Acetone is a common organic compound used in both commercial products and industrial solvents as well as a biological product of ketone body breakdown in mammals and metabolic fermentation in some anaerobic bacteria (13).Despite its toxicity, acetone is utilized as a primary carbon source in bacteria through the activity of the metal loenzyme acetone carboxylase (AC) (14).AC catalyzes the ATP-dependent reaction of bicarbonate with acetone to produce acetoacetate, which is subsequently converted into acetyl-CoA, a central metabolite (14,15).
ACs are complex six subunit (αβγ) 2 bacterial enzymes that have been reported to use either Mn(II) or Fe(II) as the divalent active site metal cofactor (16)(17)(18)(19).ACs from Xanthobacter autotrophicus and Rhodobacter capsulatus (α-proteobacteria) both contained ~1.5 mol Mn, varying amounts of Fe, and ~1 mol of Zn per (αβγ) 2 (19)(20)(21)(22).Physiological and biochemical studies showed that AC from R. capsulatus is Mn-depend ent (23).In this work, growth on increasing concentrations of Mn stimulated the activity of AC in cells and the presence of Mn could be detected in cells by electron paramag netic resonance (EPR).Further EPR studies revealed a Mn(II) signal that intensified in the presence of added nucleotide.A mechanism was proposed in which ATP coordina ted Mn(II) and successively transferred its γ and β phosphoryl groups to acetone and bicarbonate.The activated carbon substrates would then combine at the same Mn(II) center, releasing AMP and two equivalents of inorganic phosphate (24).
The first structural data obtained for the X. autotrophicus AC, however, showed a molecule of AMP bound well beyond coordinating distance at approximately 40 Å from the Mn(II) (Fig. 1).An apparently structural Zn(II) is coordinated by four cysteinyl residues in the γ subunit.AMP binding was also correlated with changes in the coordination state of the Mn(II) and the opening of a water-accessible channel connecting the nucleotide and metal binding sites.These structures led us to propose a revised mechanism, consistent with the experimentally observed reaction stoichiometry in which acetone and bicarbonate are phosphorylated by the same ATP at the ATP binding site and then travel through the enzyme's interior channel to the metal (16).Mn(II) is proposed to coordinate the phosphorylated intermediates, catalyzing C-C bond formation through a combination of inductive effects and a proton relay to the phosphate products.No experimental data describing the Mn(II)-catalyzed portion of the reaction are yet available.
AC from Aromatoleum aromaticum (a β-protetobacterium) has also been purified and reported with ~2.0 mole equivalents Fe, 1 Zn, and no Mn (18) in contrast to the reported metal content of X. autotrophicus and R. capsulatus discussed above.This came as somewhat of a surprise since the A. aromaticum AC shares >68% sequence identity with the X. autotrophicus AC.Given that the proposed role of the catalytic metal in AC can potentially be served by either Mn(II) or Fe(II), it is most surprising that there are ACs that use Mn in light of the lower thermodynamic affinity predicted by the IrvingWilliams series.
Here, we heterologously expressed the A. aromaticum and X. autotrophicus ACs in Escherichia coli under the same conditions, characterized the susceptibility of each enzyme to metal chelators, and examined metal (Mn or Fe) preference in reconstitution experiments.We observed distinct, intrinsic preferences for Mn(II) or Fe(II) for each enzyme, and correlated metal binding at the active site with specific EPR and Mössbauer spectroscopic signatures.The results suggest both an intrinsic binding preference and a specific dependence of activity in each enzyme on the correct metal.

Expression and purification of recombinant ACs
AC from X. autotrophicus and A. aromaticum were expressed under the same conditions using pET-Duet system vectors (EMD Millipore, Merck, Darmstadt, Germany) in E. coli BL21(DE3) cells grown in lysogeny broth medium supplemented with 50 µM MnCl 2 .Both enzymes were purified as previously described (16) with slight modification.The E. coli cells were lysed by sonication in a 25 mM Tris, pH 7.6, 0.1 mM ethylenediaminetetraacetic acid (EDTA), and 20% glycerol buffer.The lysate was applied to a Ni(II)-NTA (nitrilotriace tic acid) column (Bio-Rad), for purification via an N-terminal 6-histidine tag on the β subunit, and eluted with a linear gradient with an imidazole buffer (25 mM Tris, pH 7.6, 0.1 mM EDTA, 20% glycerol, and 400 mM imidazole) (16).Fractions containing AC were pooled and buffer exchanged into a 20 mM Tris, pH 7.6 buffer and applied to a Q-sepharose (Bio-Rad) column.The protein was eluted with a linear gradient with a NaCl buffer (20 mM Tris, pH 7.6, 800 mM NaCl).Fractions were pooled and exchanged into

Metal analysis via inductively coupled plasma emission mass spectrometry (ICP-MS)
Metal analysis was performed on supernatants of nitric acid digested and precipitated proteins using an Agilent Infinity II autosampler coupled to an Agilent 7800 ICP-MS.The mobile phase was 2% HNO 3 , 0.5% HCl in water with a flow rate of 1 mL/min.Signals for 55 Mn, 56 Fe, and 66 Zn were monitored for 32.8 s with an integration time/mass of 0.33 s per analyte.Quantification of analytes was performed in Agilent MassHunter 4.6 (version C.01.06) against a standard curve.Data were collected on the pooled biological replicates used for activity assays.

EPR spectroscopy
Samples (250 μL) were prepared in 50 mM Tris, pH 7.6, 150 mM NaCl buffer.For AMPcontaining samples, 10 mM AMP and 1 mM MgCl 2 were added.Final concentrations of the X. autotrophicus AC were 27 mg/mL and ~20 mg/mL, and 35 mg/mL and ~20 mg/mL for the A. aromaticum AC for the data reported in Fig. 2. Samples in quartz EPR tubes were frozen in liquid nitrogen.X-band EPR spectra were measured using a Bruker EMX continuous-wave spectrometer equipped with a continuous flow, liquid helium-cooled Oxford cryostat.EPR parameters were as follows: modulation amplitude 10 G, modula tion frequency 100 kHz, 2 mW power, temperature 12 K, and microwave frequency 9.38 GHz.Data were measured on >3 independent preparations of each enzyme, demonstrat ing the reproducibility of the spectra.Representative data are shown.

Mössbauer spectroscopy
EDTA/TEA-treated AC was reconstituted with 57 Fe(II) in an anaerobic Coy chamber.Proteins were buffer exchanged with an oxygen-and metal-free 50 mM HEPES, pH 7.6, 150 mM NaCl, and 1 mM AMP buffer. 57Fe(II) was added to a final concentration of 200 μM, the protein incubated on ice for 1 h, buffer exchanged against a 50 mM HEPES, pH 7.6, 150 mM NaCl, with or without 1 mM AMP buffer, and concentrated to 500 μL and were frozen in custom polyethylene containers.The A. aromaticum AC samples were concentrated at 44 mg/mL (AMP present) and 79 mg/mL (AMP-free).The X. autotrophicus samples were concentrated at 48 mg/mL (AMP present) and 58 mg/mL (AMP-free).
Zero-field, temperature-dependent 57 Fe Mössbauer spectra were recorded using a spectrometer operated in a constant acceleration mode.The instrument was equipped with a Janis 8DT cryostat cooled with liquid helium.Spectra recorded at 4.2 K were obtained by submerging the absorbers in liquid helium.At 80 K, we used a Cryocon 22C-120 controller and a Cernox sensor to measure the temperature of the sample which was kept in a stream of cold gas.The 14.4 keV gamma-ray source consisted of 80 mCi 57 Co dispersed in a rhodium foil.Isomer shift values are reported against the center of a room temperature spectrum recorded for a foil of α iron.Spectral simulations were performed using the WMOSS spectral analysis software (See Co., Edina, MN) and Igor Pro codes developed in-house.

Homology modeling
A homology model for A. aromaticum AC was constructed using Robetta comparative modeling (26,27).The three Mn-and Zn-bound X. autotrophicus AC crystal structures (PDB ID: 5SVC for ligand-free; 5SVB for Mg 2+ -AMP bound; 5M45 for acetate and Mg 2+ -AMP bound) were used as templates.Fe(II) was inserted in the active site, and the structure was refined by force-field-based geometry optimizations.The Amber14 force field was used for the protein.The active site was parametrized from cluster models, which include the Fe center and its ligands.The N-terminus was modeled with an acetyl group and the C-terminus with an amide group.An analogous structural model was built for the Zn binding site.From these models, atomic point charges were obtained from the standard RESP procedure using electrostatic potentials obtained from density functional theory (DFT) geometry optimization using the B3LYP (28-31) exchange and correlation functional and the def2-TZVP basis set (32).These models were also used to extract equilibrium geometrical parameters.The AMP parameters were adapted from the R.E.D.D. project database (http://upjv.q4md-forcefieldtools.org).DFT calculations were performed with NWChem6.8 (33).Force field optimizations were performed with Gromacs2022 (34,35).

RESULTS
The ACs from A. aromaticum and X. autotrophicus were expressed in E. coli and their metal contents were measured by ICP-MS.The absence of known non-specific or AC-specific chaperones implies that metal incorporation is controlled only by the affinity of the enzyme for either metal in the host cytoplasm.The AC from X. autotrophicus was isolated with roughly the same amount of Fe and Mn bound (Table 1), in spite of having an expected twofold or greater excess of Fe in the cytoplasm of E. coli cultivated in LB medium, no known Mn(II) chaperones (36), and a thermodynamic bias toward Fe(II) binding predicted by Irving and Williams (15).The heterologously expressed X. autotrophicus had comparable Fe content and about twofold lower Mn content than previously reported for the enzyme isolated from the native host (19).It was further observed that the Mn incorporation is dependent on media composition in the native host culture when more defined mineral salt media were used (23).For heterologous expression, we are somewhat constrained in terms of media composition given that rich media is important for maximal protein expression.In contrast to what is observed for X. autotrophicus AC, the metal complement of A. aromaticum AC is almost entirely Fe with an order of magnitude lower detected Mn.As in the case of X. autotrophicus AC, we observed about twofold lower Fe in A. aromaticum AC in our heterologously expressed b Percent specific activity is equal to the amount of activity in nmol NADH min −1 mg −1 (measured via the NADH dehydrogenase-coupled assay using UV/visible spectroscopy) recovered after EDTA/TEA treatment and metal reconstitution compared to untreated protein, expressed as a percentage of the initial, untreated specific activity (=100%).Numbers are averages of three measurements made on samples of independently expressed/isolated enzymes, and errors are ±1 standard deviation.The activity was determined by monitoring acetoacetate production via a coupled assay.See Materials and Methods.
samples than what was observed for A. aromaticum AC isolated from the native host (18).Again, the somewhat lower yield could relate to suboptimal expression conditions in comparison to the defined medium used to culture the native host.Irrespective of the observed difference in the magnitude of metal loading in the two ACs the data are unambiguous in confirming that the two ACs bind metals differently, that A. aromaticum AC has a very strong preference for binding Fe, and that the differences in metal content of the X. autotrophicus and A. aromaticum ACs cannot be attributed to growth conditions, expression conditions, or any factors inherent to the host physiology.In addition, the A. aromaticum AC exhibited a greater than twofold higher specific activity than was observed for X. autotrophicus AC as determined in the 3-hydroxybutyrate dehydrogenase coupled assay.
It was previously reported that the Mn associated with X. autotrophicus AC was recalcitrant to chelation (23).We therefore investigated the susceptibility to metal chelators EDTA and TEA for X. autotrophicus and A. aromaticum ACs side-by-side.In contrast to what was observed previously, we were able to remove a large fraction of metals (Fe, Mn, and Zn) from X. autotrophicus AC (Table 1).Interestingly, the loss of metals resulted in a loss of only about 25% of the activity (Table 1).The simplest explanation for this is that a proportion of our enzyme preparation that had bound Mn was inactive and that the active X.autotrophicus AC is somewhat recalcitrant to the chelation of Mn as previously reported (23).Treating the A. aromaticum AC with the same chelation conditions also resulted in a large proportion of the metals being removed, however, activity was almost completely eliminated.This contrasts with what was observed for X. autotrophicus AC and suggests that A. aromaticum AC is more susceptible to chelation.For both ACs, a large proportion of the protein was lost under these conditions which we had optimized to observe chelation.
To provide further insights into metal preference and metal dependency, we attempted to reconstitute the remaining X. autotrophicus AC and A. aromaticum ACs with both Fe and Mn.Additional protein was lost in this procedure and given the manipulations, the data can only be treated qualitatively though they do reveal some key insights.In addition, although steps were taken to remove metals that are not bound, we cannot directly determine the amount of metal that is bound adventitiously somewhere other than the active site.For X. autotrophicus AC, reconstitution with Mn only resulted in 0.25 stoichiometric equivalents of metal per active site but restored the extent of the activity that was lost in chelation.Reconstitution of X. autotrophicus AC with Fe resulted in 0.7 equivalents of metal per active site but only a very small amount of activity was restored.The small amount of activity restored could have been a result of a small amount of Mn contaminating the Fe reconstitution since the Mn concentration is observed to increase twofold in this experiment.Slight Fe contamination was also observed in the X. autotrophicus AC reconstitution.For A. aromaticum AC, the reconstitu tion results were similar regarding incorporation, with Mn reconstitution yielding 0.25 equivalents for Mn and 0.70 equivalents for Fe.The activity data were much clearer, largely as a result of more effective chelation where the reconstitution with Mn resulted in no restoration of activity and reconstitution with Fe resulted in complete restoration of activity.Interestingly, 100% of the activity was restored without any addition of Zn, indicating Zn is not important for activity.
The results confirm the metal dependencies inferred by the two independent research groups studying the X. autotrophicus and A. aromaticum ACs isolated in their native hosts.Furthermore, the results indicate that the addition of Fe will not supplant the role of Mn in X. autotrophicus and Mn will not supplant the role of Fe in A. aromati cum.
To ensure these metal removal and reconstitution experiments were associated with at least a proportion of the metal-bound at the active site, we examined each AC for components in the Mn(II) EPR spectra that could be diagnostic of metal bound to the active site.Prior work suggested this may be the case.The first X-band EPR spectra measured for an AC (from R. capsulatus) showed a weak Mn(II) signal which intensified when Mg-AMP or Mg-ATP were added (36).Previously, we solved a series of crystal structures for the related X. autotrophicus AC ( 16), revealing Mn(II) coordinated by H150, H175, and D153 in the α subunit and a presumed structural Zn in the γ (Fig. 1).
Surprisingly, the AMP binding site was in the β subunit, about 40 Å from the Mn site, and clearly too far to directly induce the spectral changes observed by Mn(II) EPR.However, compared with the non-liganded AC, the AMP-bound structure featured a newly opened interior pocket connecting the nucleotide and metal binding sites.This large-scale structural change was accompanied by rotation of the side chain of E89 into bidentate coordination with Mn(II), which went from 5-(H150, H175, bidentate D89, and water, not shown) to 6-coordinate (Fig. 1) (16).The intense 6-line EPR spectrum observed for this species but not for the as-isolated enzyme is consistent with symmetric/octahe drally coordinated Mn(II) with an I = 5/2 nuclear spin.
The EPR spectrum measured for the expressed X. autotrophicus AC consisted of a weak, irregular signal centered near g = 2, similar to spectra measured previously for the AC from R. capsulatus (36).Upon the addition of MgAMP, the apparent Mn(II) signal sharpened and intensified, revealing a clearly discernable 6-line hyperfine pattern consistent with octahedrally coordinated Mn(II).An intense, isotropic signal near g = 4.5 was also observed, suggestive of Fe(III) (S = 5/2) in a symmetric average ligand environment, though no Fe was observed in the crystal structure for the X. autotrophicus AC.The Fe(III) signal did not change with the addition of AMP.We note that Fe(II) (d 6 ) (S = 0 or S = 2) is undetectable under normal X-band EPR conditions.Hence, we would not expect EPR to report on protein-bound Fe(II).
Following dialysis against EDTA/TEA, we observed a pronounced decrease in the intensity of the g = 2 signal, and a more modest decrease at g = 4.5.These results suggested that the EPR-observable Fe(III), which was easily removed by dialysis against chelators, contributed to the total iron associated with the X. autotrophicus AC (Fig. 2; Table 1).Reconstituting this AC with Mn(II) in the presence of AMP resulted in an intense 6-line signal, indicative of Mn(II) bound in an octahedral environment at the active site, along with an increase in the g = 4.5 signal associated with the weakly bound Fe(III) (Fig. 2).A similar pattern was observed for the A. aromaticum AC, in which reconstitution with Mn(II) resulted in the same 6-line signal associated with active site bound Mn(II).This finding suggests that both enzymes undergo an AMP-dependent change in their coordination, and both are capable of binding Mn(II) in their active sites.A symmetric signal at g = 4.5 may likewise be associated with non-catalytically-active Fe(III) in the A. aromaticum AC.The iron fraction responsible for this signal is markedly less susceptible to removal by chelation and may account for the iron detected in this AC after dialysis (Table 1).We concluded that EPR could apparently be used to detect active-site associated Mn(II) in both ACs.An increase in this signal's intensity correlated with the increase in enzymatic activity observed for the Mn(II)-depleted X. autotrophicus AC following reconstitution with Mn(II).While heterogeneity in the expressed, hetero hexameric protein made it impossible to quantify the correlation between activity and active site Mn(II) occupancy on a per-metal basis, the trend was clearly and reproducibly observed.
The reciprocal relationship between activity and active site iron could not be examined using X-band EPR, as Fe(II) lacks a signal.We consequently examined whether 57 Fe-Mössbauer spectroscopy, which is highly sensitive to iron oxidation state and coordination geometry (37), would reveal analogous AMP-dependent changes in coordination for 57 Fe(II)-bound to AC. Mössbauer samples were prepared in a similar way to those used for EPR, except that excess dithionite was added to reduce all of the AC-associated iron to Fe(II).We hypothesized that at least two populations of Fe(II) sites would be present: one corresponding to the active site metal, and another non-catalytic iron pool more sensitive to chelation.
The spectrum of the expressed A. aromaticum AC was deconvoluted into four quadrupole doublets (Fig. 3; Table S1) three of which (blue, red, and orange) were affected by the addition of AMP and which originated from high spin (S = 2) Fe(II) sites.
The fourth quadrupole doublet (green) was traced to an instrumental artifact, namely, to an iron impurity in the window of our gamma ray detector, and is observed for all samples investigated in this study.We hypothesize that the AMP-induced conversion of the blue doublet to the red and orange components accounts for the transition from the 5-coordinate active site (blue) to the 6-coordinate active site Fe(II) with Glu coordinated (red and/or orange).It is unclear why would there be two doublets (red and orange) for the 6-coordinate site, but it may be explained by the presence of different, but related coordination conformations.As of now, we do not have an experimentally determined structure and we are basing our hypotheses strictly on the analogies to X. autotrophicus AC.
The Mössbauer spectra of the X. autotrophicus protein exhibited two AC specific high spin (S = 2) Fe(II) quadrupole doublets (red and blue, Fig. 3; Table S1) which are affected by the AMP binding.In contrast to what is observed for A. aromaticum AC, for X. autotrophicus AC we observed only a small increase in the contribution of the red doublet with very little change in the blue component.The changes induced by the binding of AMP suggest that some Fe(II) is likely bound at the active site.However, the lower magnitude of the change in the red component, little or no change in the blue component, and the absence of the orange doublet observed for A. aromaticum AC, suggests that Fe(II) is bound differently in the two ACs such that the binding of AMP does not invoke the same changes in coordination.This is in line with the reconstitution experiments that indicate in sum that metals are bound differently in the active sites of A. aromaticum and X. autotrophicus ACs.

DISCUSSION
Thermodynamically driven metalation should favor incorporation of Fe(II) into ACs.Yet, we have demonstrated here that two homologous carboxylases with identical first coordination spheres nonetheless exhibit distinct binding preferences for Fe(II) or Mn(II).The preference in the case of Mn(II) goes against the predicted order of the Irving-Wil liams series (10-12) and is even observed for the X. autotrophicus enzyme produced in iron-rich media and in an E. coli environment where Mn(II) and AC-specific chaperones are absent.The distinct binding preferences are all the more remarkable given the strict catalytic dependence of each enzyme on only its cognate metal as demonstrated here, though either Mn(II) or Fe(II) could in principle serve the proposed role as a Lewis acid in catalysis.
A preponderance of evidence has led to a set of principles for understanding metal preferences in the O 2 -activating Mn/Fe binding sites of class I ribonucleotide reducta ses and Mn/Fe ligand-binding oxidases (12,(38)(39)(40).These invoke biological constraints leading to metal selectivities such as pre-organized first coordination spheres that favor Mn(II) (octahedral) or Fe(II) (tetrahedral), the participation of one metal to template incorporation of the second, or specific side-chain based post-translational modifications that prohibit entry of one metal or the other.Recent work with designed metalloproteins that incorporate metals with preferences that do not follow the Irving-Williams series order do so through the participation of two metal binding sites, one of which dynami cally biases the conformation of the protein to bind lower Irving-Williams metals in the second site, preferentially over Cu(II) (41,42).The mononuclear sites in the ACs, however, lack either a pre-organized, Mn(II)-favoring ligand set or post-translational modifications.They also cannot make use of an adjacent metal site to drive metal binding cooperatively (43).
Instead, metal selectivity in the mononuclear sites of the ACs must be specified outside the conserved immediate coordination environment of the metal.To gain some hypothetical understanding of how selectivity could work, we compared the available crystallographic structures for the X. autotrophicus AC to models of the A. aromaticum AC, which were constructed and energy minimized using comparative modeling of the fully solvated enzyme with the X. autotrophicus AC structures as a template.Comparisons of these structures highlight several differences in their outer coordination spheres, any of which could be involved in encoding metal preferences.For example, a patch of aromatic residues in the X. autotrophicus AC (Trp401, Trp479, Trp408, and Trp438) adopts a mixed stacked/T-shape arrangement that limits water access to the active site (Fig. 4).Such a hydrophobic obstacle course could preferentially favor passage of Mn(II) over Fe(II), because of the relative ease of exchanging solvent water for active site ligands by Mn(II).A similar patch of aromatic residues in A. aromaticum AC (Trp398, Trp476, Trp405) adopts a significantly more ordered stacked arrangement, granting easier water access to the active site.Understanding the protein-based selectivity filters in these enzymes will advance our ability to emulate nature's precise positioning of metals in complex systems.The observed selectivity also suggests a means, under the control of natural selection, by which an aerobic species (X.autotrophicus) could adapt an enzyme to utilize Mn(II).Such adaptation could be beneficial in the face of the decreased Fe(II) availability and the rise of harmful Fe(II)/O 2 Fenton reactivity accompanying the oxygenation of the atmosphere.

FIG 1
FIG 1 Structure and reaction of AC. (A) Crystal structure of X. autotrophicus AC with AMP (red) bound.The α subunit, containing the Mn(II) active site, is colored in teal, the β subunit with the nucleotide-binding site is colored in blue, and the Zn(II) containing β subunit is colored in wheat.One set of αβγ subunits is transparent to show the Mn, AMP, and Zn bound.(B) Comparison of the active sites of X. autotrophicus AC (teal carbons) and the Aromatoleum aromaticum AC homology model (wheat carbons) without substrates, products, or their analogs bound.Following nucleotide binding, E89 (E86 in the homology model, residues labeled in black) rotates in coordination with the metal.(C) In the X. autotrophicus catalyzed reactions, the γ-phosphoryl (purple) is proposed to be transferred to acetone (green) and the β-phosphoryl to bicarbonate to generate the phosphoenolacetone and carboxyphosphate intermediates, respectively, at the ATP binding site.(D) The two phosphorylated intermediates are hypothesized to travel 40 Å to the Mn(II) site where C-C bond formation occurs.An active site base and divalent metal catalyze the release of CO 2 , which reacts with the phosphoenolacetone to form the acetoacetate product.

FIG 2
FIG 2 EPR spectra measured for AC from A. aromaticum (top) and X. autotrophicus (bottom) reconstituted with Mn(II).Spectra for metal-depleted enzymes are shown in purple, along with enzymes reconstituted with Mn(II) (blue) and Mn(II) plus AMP (red).Regions of the spectra corresponding to Fe(III) (left) and Mn(II) (right) are shown on different intensity (y-axis) scales.The 6-line spectrum is associated with Mn(II) in a 6-coordinate active site.This is proposed to form following AMP binding and repositioning of the E89 side chain in the X. autotrophicus AC structure (E86 in the AC from A. aromaticum).Protein concentrations were initially (purple traces) 73 μM (top) and 98 μM (bottom).Following reconstitution with Mn(II) (±AMP): 55 μM (top) and 48 μM (bottom).EPR conditions: 12 K, modulation amplitude 10 G, modulation frequency 100 kHz, 2 mW power, and microwave frequency 9.38 GHz.Data were measured on >3 independent preparations of each enzyme.Representative data are shown.

FIG 3
FIG 3 Mössbauer spectra of A. aromaticum AC or X. autotrophicus AC reconstituted with 57 Fe(II).Spectra, from the top, are as follows: A. aromaticum AC, A. aromaticum AC in the presence of AMP, X. autotrophicus AC in the absence of AMP, and X. autotrophicus AC in the presence of AMP.Spectra were measured at 80 K, 0T (A.aromaticum AC) or 4.2 K, 0T (X.autotrophicus AC).Simulated Fe(II) signals are solid color lines while experimental data are shown in black.The solid gray line is the sum of all the individual spectra.The blue simulated doublet is attributed to the Fe(II) site, the red doublet to the glutamate-bound Fe(II) induced when AMP is bound, and the orange doublet to an unknown Fe(II) state.The green doublet is attributed to an instrumental artifact originating from an iron impurity in the window of our detector.

FIG 4
FIG4 Proposed metal selectivity filter.The refined structures of X. autotrophicus (green) and A. aromaticum (tan), indicating possible differences in access to the metal (purple or rust spheres, respectively), are shown.In the X. autotrophicus structure, the three Trp resides (W401, W408, and W479) are arranged in a mixed stacked/T-shape that restricts solvent access to the Mn site.In the A. aromaticum model, however, the W398 and W479 residues are more ordered and stacked.Water molecules are shown as sticks.