Inhibitors of the Histone Methyltransferases EZH2/1 Induce a Potent Antiviral State and Suppress Infection by Diverse Viral Pathogens

ABSTRACT Epigenetic regulation is based on a network of complexes that modulate the chromatin character and structure of the genome to impact gene expression, cell fate, and development. Thus, epigenetic modulators represent novel therapeutic targets used to treat a range of diseases, including malignancies. Infectious pathogens such as herpesviruses are also regulated by cellular epigenetic machinery, and epigenetic therapeutics represent a novel approach used to control infection, persistence, and the resulting recurrent disease. The histone H3K27 methyltransferases EZH2 and EZH1 (EZH2/1) are epigenetic repressors that suppress gene transcription via propagation of repressive H3K27me3-enriched chromatin domains. However, while EZH2/1 are implicated in the repression of herpesviral gene expression, inhibitors of these enzymes suppressed primary herpes simplex virus (HSV) infection in vitro and in vivo. Furthermore, these compounds blocked lytic viral replication following induction of HSV reactivation in latently infected sensory ganglia. Suppression correlated with the induction of multiple inflammatory, stress, and antipathogen pathways, as well as enhanced recruitment of immune cells to in vivo infection sites. Importantly, EZH2/1 inhibitors induced a cellular antiviral state that also suppressed infection with DNA (human cytomegalovirus, adenovirus) and RNA (Zika virus) viruses. Thus, EZH2/1 inhibitors have considerable potential as general antivirals through the activation of cellular antiviral and immune responses.

F ollowing primary infection with herpes simplex virus (HSV), the virus establishes lifelong latency in sensory neurons. However, multiple biological or stress stimuli can induce reactivation of latent genomes and recurrent disease. Ocular HSV infections remain the leading virus-mediated cause of stromal keratitis and corneal scarring, while neonatal infections can result in death, developmental delays, or persisting neurological issues (1,2). In addition to these pathologies, HSV-2 infection is linked to enhanced acquisition and transmission of HIV (3)(4)(5).
The most widely utilized antiherpetic pharmaceuticals target the viral DNA polymerase to interfere with late-stage viral replication. However, drug-resistant strains emerge (6,7), particularly in immunocompromised individuals (8). Furthermore, these compounds do not adequately control subclinical infectious viral shedding, which is the most prevalent means of transmission.
Many DNA viruses, including herpesviruses, are subject to epigenetic regulation where productive infection, persistence, and quiescence/latency are determined, in part, by the modulation of chromatin associated with the viral genomes (9)(10)(11)(12)(13)(14). Epigenetic regulation is mediated by families of histone modification enzymes, adaptor recognition proteins, and chromatin remodelers. Thus, components of the cellular epigenetic machinery represent a plethora of novel therapeutic targets that can be used to modulate the expression of specific gene sets and alter the course of disease. Inhibitors of LSD1 and members of the JMJD2 family of histone H3K9 demethylases have been shown to suppress HSV infection and reactivation from latency (15)(16)(17)(18). In a contrasting approach, histone deacetylase inhibitors have been components of some strategies to induce HIV reactivation and deplete latent viral reservoirs (19)(20)(21).
Here, in contrast to the anticipated suppressive role of EZH2/1, inhibition of these histone methyltransferases results in reduced HSV gene expression and lytic infection in vitro and in vivo. Additionally, inhibitors did not induce reactivation but rather suppressed the spread of viral reactivation in a ganglion explant model. Investigation of the mechanisms involved in the antiviral activities of these inhibitors revealed that treatment induces multiple components of antipathogen pathways that result in an enhanced cellular antiviral state. Importantly, the antiviral effects are not limited to HSV but also extends to other representative nuclear DNA viruses (human cytomegalovirus (H3K9me3, H3K27me3) are assembled on the viral genome. To investigate the contribution of EZH2/1-catalyzed H3K27 methylation to suppression of viral gene expression during lytic infection, primary human foreskin fibroblast (HFF) cells were pretreated with the EZH2/1 catalytic inhibitors GSK126, GSK343, and UNC1999 (25,26,29) for 5 h and infected with HSV-1 (Fig. 1A to D and F). Strikingly, in contrast to the anticipated enhancement of viral gene expression, each compound suppressed viral IE gene transcription in a dose-dependent manner. Similarly, treatment with astemizole, a compound that interferes with the association of EZH2/1 with the chromatin binding subunit of the PRC2 complex (EED, embryonic ectoderm development) (24), also suppressed viral IE gene expression ( Fig. 1A and E to F). Thus, suppression of HSV gene expression was dependent on either inhibition of the catalytic activity of EZH2/1 or prevention of the assembly of the PRC2 complex. Pretreatment of cells with GSK126 for 1 h was sufficient to reduce viral IE expression, while recovery of suppressed viral IE gene expression could be rescued within 6 to 12 h following washout of the compounds (see Fig. S1A to C in the supplemental material).
To determine if EZH2/1 inhibitors would suppress viral lytic growth and spread, cells were infected with HSV-1 for 8 h to allow one round of replication and subsequently treated with the vehicle, GSK126, GSK343, or the viral DNA replication inhibitor acyclovir (ACV) for 12 h. As shown by immunofluorescent staining for the viral DNA replication protein UL29/ICP8, treatment with each compound potently suppressed productive viral spread from the initial infected cells (Fig. 1G) and decreased viral yields by 2 to 3 logs (Fig. 1H).
EZH2/1 inhibitors reduce the number of transcriptionally active viral genomes. Suppression of HSV-1 IE gene expression was not due to a block in the transport of viral genomes to the nucleus and was relatively independent of the multiplicity of infection (MOI) (Fig. S1D and E). However, the suppression of viral IE gene expression suggested that EZH2/1 inhibitors acted at an early stage of infection. Therefore, cells were treated with the vehicle, GSK126, or UNC1999; infected with HSV-1 for 1.5 h; and stained for a marker of active HSV-1 transcriptional foci (ICP4). As shown in Fig. 2A to D, treatment with either drug reduced the number of transcriptionally active viral genomes per cell. Suppression of IE transcription was further supported by chromatin immunoprecipitation (ChIP) assays, which demonstrated a reduction in the levels of the IE coactivator HCF-1, RNA polymerase II (RNAPII), and the euchromatic marker H3K4me3 associated with the viral IE-ICP0 promoter (Fig. 2E). In contrast, no significant change in occupancy of the control cellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter was evident. Therefore, in stark contrast to the anticipated effect, inhibition of EZH2/1 resulted in reduced IE gene expression, decreased numbers of transcriptionally accessible viral genomes, and suppression of lytic infection. EZH2/1 inhibitors induce a cellular antiviral state. These data suggested that EZH2/1 inhibitors might induce an antiviral state that results in suppression of the viral genome. In support of this, the number of promyelocytic leukemia (PML) foci, a marker of stress-and pathogen-induced cellular responses (51), was higher in GSK126-and UNC1999-treated cells than in vehicle-treated cells ( Fig. 3A and B). Furthermore, interferon-alpha (IFN-␣) and interleukin-8 (IL-8) mRNAs were significantly upregulated in GSK126-treated cells (Fig. 3C), indicating that EZH2/1 inhibitors might mediate viral repression via IFN/immune signaling-related pathways. To directly address this, the mRNA levels of a selection of IFNs/IFN receptors were measured in GSK126-treated cells with a quantitative PCR (qPCR) Profiler array ( Fig. 3D; Table S1A). Compared to vehicle, treatment of cells with GSK126 enhanced the levels of nine genes (Ͼ1.5-fold; P Ͻ 0.05), including those encoding IL-6, the IL-6 receptor, IFN-␣1, and IFN-␣2.
Global differentials in gene expression were next assessed by microarray analyses of HFF cells treated for 4 h with GSK126 or GSK343 (Table S1B) Table S1B). Furthermore, of the 87 genes differentially regulated by both inhibitors, 61 (71.1%) were ISGs. Ingenuity pathway analyses (IPAs) of the differentially regulated genes ( Fig. S2A and B; Table S1B) supports the induction of proinflammatory and stress response pathways in both GSK126-and GSK343-treated cells.
As shown in Fig. 3F and Table S1B, enriched pathways identified by IPA were grouped into six supercategories that illustrate the modulation of immune signaling and cellular antiviral pathways in inhibitor-treated cells. A subset of the genes induced in the qPCR arrays and microarrays could be further organized into a functional network. In this network, IFNs (IFNA1/2, IFNAR1), inflammatory cytokines and mediators (IL-6, IL-8, PTGS2), and transcription factors that induce the expression of inflammatory and endoplasmic reticulum (ER) stress response factors (CEBPB, Fos, ATF4, DDIT3) are nodes with functional relationships (STRING [53]) that illustrate the modulation of a broad spectrum of antipathogen pathways by EZH2/1 inhibitors (Fig. 3G). These results are consistent with previous observations in cancer cell lines that depletion of SUZ12, a subunit of the EZH2/1-PRC2 complex, resulted in the expression of a class of ISGs whose promoters exhibited bivalent (H3K27me3 and H3K4me3) histone methylation markers (54). The results are also complemented by analyses of data sets (GEO data set GSE81267) published by Qi et al. (28) where treatment of Karpas422 cancer cells with a novel inhibitor targeting the PRC2 subunit EED (EED226) induced antipathogen gene cascades that parallel the inflammatory and stress pathways shown here ( Fig. S2C and D; Table S1C). Thus, inhibition of EZH2/1 results in the differential regulation of ISGs and components of cellular defense pathways in a manner dependent on EZH2/1 catalytic activity and PRC2 complex integrity.
To define the kinetics, transcriptome sequencing (RNA-Seq) analyses were performed with cells treated with GSK126 for 1, 2, or 5 h (Table S1D). Strikingly, the results support the induction of antipathogen response pathways by 2 h, with significant enhancement of these pathways by 5 h (Fig. 3H; Fig. S2E and Table S1D). Components induced by 2 h formed a functional network enriched in signaling molecules (IL-6, mitogen-activated protein kinase kinase kinase 8 [MAP3K8], SGK1/3) and transcription factors (Fos, CEBPB/CEBPD, DDIT3, NR4A1/2, ATF3) that are regulators of antiviral and stress response pathways (Fig. 3I). Importantly, although EZH2/1 inhibitors induced IFNs and ISGs, GSK126 treatment of cells lacking the type 1 IFN receptor (IFNAR1 Ϫ/Ϫ ) still prevented the spread of HSV infection (Fig. S3). Thus, suppression is mediated in a manner that does not require IFNAR1 signaling. The induction of transcription factors and signaling molecules by EZH2/1 inhibitors suggests that these compounds induce critical components of antiviral pathways that are downstream of direct type I IFN receptor signaling.

EZH2/1 inhibition suppresses the infection of multiple viral pathogens.
On the basis of the regulation of multiple antiviral components and pathways by EZH2/1 inhibitors, it was hypothesized that these compounds would function as broadspectrum antiviral inhibitors. Indeed, GSK126 and GSK343 both suppressed the IE gene expression of two other nuclear DNA viruses, Ad5 and hCMV (Fig. S4). To determine if these inhibitors would suppress an RNA virus with a distinct life cycle, HFF cells were treated with GSK126 and infected with ZIKV, a member of the Flavivirus family. As shown in Fig. 4A to C, GSK126 significantly reduced both the number and size of ZIKV focus-forming units (FFU; plaques) in a dose-dependent manner. These results were further supported by a GSK126-meditated reduction in the number of ZIKV-infected cells at 1 and 2 days postinfection (dpi), as measured by intracellular staining for ZIKV antigens (Fig. 4D). Finally, while pretreatment was modestly more efficient at suppression of infection at lower GSK126 concentrations, it was clearly not essential to effect significant suppression (Fig. 4E).

EZH2/1 inhibitors suppress primary HSV infection and enhance the recruitment of immune cells to the site of infection in vivo.
Given the suppression of HSV-1 gene expression and lytic infection in culture, as well as the regulation of antiviral signaling, the impact of EZH2/1 inhibitors on HSV-1 ocular and intranasal infections was assessed in vivo. BALB/c mice were infected via the ocular route and treated topically with the vehicle, the viral DNA replication inhibitor ACV, or EZH2/1 inhibitors for 7 dpi. As shown in Fig. 5A, GSK126 or UNC1999 treatment significantly reduced the progression of infection, as measured by ocular viral yields (infectious virus titers) and viral DNA loads. Suppression of ocular infection by topical GSK126 treatment was evident as early as 3 dpi, with a significant decrease in the infectious virus titer per eye at 5 dpi (Fig. 5B). In mice infected via the intranasal route, intraperitoneal (i.p.) injection of GSK126 also significantly reduced viral loads in nasal tissues (Fig. 5C).
EZH2/1 inhibitor treatment resulted in the induction of antiviral signaling pathways in vitro and the suppression of primary infection in vivo. To characterize drug-induced changes in cellular immunity, neutrophil recruitment to the site of HSV infection was assessed. Neutrophils are typically the first immune cells to migrate into infected tissues, and neutropenic individuals suffer from recurrent HSV infections. Mice were EZH2/1 Inhibitors Suppress Diverse Viral Pathogens ® mock infected or infected with HSV-1 via the ocular route and treated topically with GSK126, ACV, or the vehicle for 7 days. Ocular sections were costained to detect HSV-1-infected cells and neutrophils. Strikingly, while neutrophils were modestly recruited in the vehicle-or ACV-treated mice, treatment with GSK126 resulted in a clear increase in these cells within the cornea, iris, and anterior chamber of the infected eyes ( Fig. 5D; Fig. S5). Importantly, enhanced neutrophil recruitment was dependent on viral infection and was not evident in mock-infected mice.
Reduction in HSV-1 reactivation-spread correlates with stimulation of immune signaling pathways in mouse ganglia. Explantation of latently HSV-1-infected sensory ganglia into culture promotes induction of viral reactivation. To determine if EZH2/1 inhibitors would suppress reactivation, ganglia from latently infected mice were explanted in the presence of the vehicle, ACV, or EZH2/1 inhibitors for 48 h. GSK126, GSK343, and astemizole each reduced viral yields, as measured by infectious titers and viral DNA loads ( Fig. 6A; Fig. S6A).
Reduced viral yields can result from suppression of the initial primary reactivation events and/or suppression of the spread of reactivated virus in the ganglia. To discrim- inate between these, latently infected ganglia were explanted in the presence of the vehicle, ACV, GSK126, or GSK343 for 48 h and sections were stained for the viral lytic replication protein UL29 (ICP8) (Fig. 6B to D). Ganglia explanted in the presence of the viral DNA replication inhibitor ACV served as comparative controls, as this compound does not suppress the primary reactivation events (single UL29 ϩ cells) but blocks viral replication and spread from the initiating neuron (clusters of UL29 ϩ cells). As shown in Fig. 6C and D, slightly lower numbers of individual/single UL29 ϩ neurons (primary reactivation events) were seen in the presence of GSK126 (7.7/ganglion) and GSK343 (7.6/ganglion) than in the presence of ACV (10.7/ganglion). However, the primary impact of these compounds was clearly in reducing the spread of the infection from the initiating neurons to adjacent cells (clusters) in a manner comparable to that of ACV.
As shown during lytic infection of cultured HFF cells, suppression of viral reactivation-spread in ganglion explants was likely to be due to EZH2/1 inhibitor modulation of antiviral signaling. This was confirmed by RNA-Seq analyses of genes differentially regulated in mock-infected and HSV-1-infected ganglia explanted for 12 h in the presence of GSK126 relative to the vehicle (Table S1E). In mock-infected ganglia, GSK126 treatment induced 13 ISGs, including that for CXCL1, while 24 ISGs, including those for CXCL1-3, CXCL5, CSF2-3, CCL2, LIF, IL-11, and IL-6, were induced in HSV-1infected ganglia (Fig. S6B; Table S1E). Thus, analogous to the enhanced recruitment of neutrophils by GSK126 in HSV-infected eyes relative to that in mock-infected eyes ( Fig. 5D; Fig. S6), the stimulation of these antiviral pathways is also enhanced in HSV-infected ganglia compared to that in mock-infected ganglia.
Pathway, Interferome, and STRING analyses clearly illustrate the induction of proinflammatory and immune cell recruitment pathways by GSK126 (Fig. 6E and F; Fig. S6C Table S1E) in explanted ganglia. While induction of IL-6, IL-8 (CXCL1/2), IL-11, PTGS2, MMP3, and LIF was common to both the HFF and ganglion RNA-Seq data sets (Table S1D and E), a more extensive set of chemokines (CXCL5, CCL2, CXCL3), adhesion molecules (SELP, ICAMI, SELE), and factors required for hematopoiesis and maturation (CSF2, CSF3) was induced in the ganglion tissue. It is likely that this differential stems from the more diverse cell population, including resident immune cells, in the infected ganglia in vivo relative to the clonal nature of the HFF cultures. Furthermore, the induction of the neutrophil chemotactic factors CXCL1, CXCL2, CXCL3, and CXCL5 is consistent with the robust recruitment of these cells to the sites of ocular HSV-1 infection (Fig. 5D; Fig. S5).

DISCUSSION
Small-molecule compounds targeting components of the epigenetic machinery hold significant promise as novel approaches to control diseases ranging from malignancies and developmental disorders to acute or chronic viral infections. Recently, inhibitors of the histone H3K27 methyltransferases EZH2/1 have been developed on the basis of the potential to treat some diffuse large B-cell, follicular, and non-Hodgkin's lymphomas (23,(55)(56)(57). Importantly, pharmacological inhibition of EZH2/1 has shown promising results in tumor regression in cancer cell lines, mouse tumor xenograft models, and human phase I trials (7,23,(25)(26)(27)(28)(29)(30)58).
Many viruses, including herpesviruses, are subject to regulation by the cellular epigenetic machinery. EZH2/1 complexes have been linked to the control of lytic and latency stages of multiple herpesviruses, including HSV, Kaposi's sarcoma-associated herpesvirus, Epstein-Barr virus, and hCMV, where these complexes and the associated H3K27me3 marks repress viral lytic gene expression. For HSV, both the H3K9me3 and H3K27me3 heterochromatic markers are associated with the genome upon lytic infection and likely represent an initial host cell antiviral response. Similarly, in sensory neurons, H3K9me3 and H3K27me3 marks and PRC complex subunits are associated with the latent HSV-1 genome and have been hypothesized to play a role in the establishment and/or maintenance of latency.
Specific epigenetic inhibitors can modulate HSV lytic infection, latency, and reactivation. Inhibitors of the histone H3K9 demethylases (LSD1, JMJD2) block initiation of HSV infection, while inhibitors of the H3K9 and H3K27 demethylases (UTX/JMJD3) block reactivation from latency. Most strikingly, inhibition of LSD1 in vivo enhances epigenetic repression of the latent HSV genomes, which correlates with a reduction of reactivation and viral shedding (15). Here, treatment of primary cells with EZH2/1 inhibitors suppressed HSV gene expression, decreased the spread of the infection to adjacent cells, and blocked the spread of viral reactivation in latently infected sensory ganglia. In vivo, EZH2/1 inhibitors suppressed primary HSV infection and enhanced immune cell infiltration at the site of infection. Thus, despite the fact that the PRC2 complex plays roles in the suppression of HSV lytic gene expression and the promotion of latency, the data presented here support a role for EZH2/1 inhibitors in the induction of an antiviral state that is clearly dominant over the loss of any direct PRC2 suppression of the HSV genome.
Collectively, the genes induced by these inhibitors compose a multifaceted antiviral state where 40.6 to 70.9% of the differentially regulated genes are ISGs. Furthermore, in addition to HSV, EZH2/1 inhibitors suppressed infection with other nuclear DNA viruses (hCMV, adenovirus), as well as ZIKV, a member of the Flavivirus family. Thus, EZH2/1 complexes are clearly critical modulators of cellular antipathogen defense pathways. It is, however, important to note that in addition to those factors involved in the stimulation of immune or antipathogen responses, EZH2/1 inhibitor treatment also resulted in the induction of genes that are involved in feedback pathways that limit excessive stress and inflammatory signaling through dampening of receptor signaling (i.e., SOCS2, IL-1RN, DUSP1), transcriptional repression (i.e., ATF3, MAFG), destabilization of AU-containing mRNAs encoding inflammatory proteins (i.e., ZFP36), and processing of reactive oxygen species (i.e., OXR1).
Many viruses have evolved mechanisms to circumvent cellular antiviral strategies and IFN responses. However, the pathogens tested here do not efficiently escape the multiple ISGs and antiviral pathways induced by EZH2/1 inhibitors. Furthermore, while the EZH2/1-PRC2 complex plays roles in the repression of herpesviruses, the impacts of EZH2/1 inhibitors in vivo are more complex and include the induced recruitment of antiviral immune cell populations. Importantly, the impacts of EZH2/1 inhibitors in vivo were clearly enhanced in infected tissues and animals relative to those in uninfected controls. Thus, these inhibitors amplify cellular antiviral responses and contribute to the induction of immune cell responses in a context-dependent manner. The results are analogous to the synergistic/enhanced induction of ISGs in cells treated with both EZH2/1 inhibitors and IFN-␥, lipopolysaccharide, IL-1␤, or tumor necrosis factor alpha relative to treatment with each compound alone (54).
Immune surveillance in latently infected sensory ganglia contributes to the suppression of HSV reactivation (59)(60)(61)(62)(63). Resident T cells and delivery of IFNs to neurons undergoing viral reactivation repress lytic induction while also preserving neuronal survival. In explanted ganglia, EZH2/1 inhibitors suppressed productive reactivation and spread of HSV from the initiating neurons. Thus, while speculative, it is possible that immune suppression responses could be enhanced by compounds such as EZH2/1 inhibitors, resulting in a decrease in productive clinical reactivation and viral shedding.
Strikingly, although PRC2 components repress herpesvirus gene expression, the induction of antiviral pathways by EZH2/1 inhibitors clearly drives immune-mediated suppression of viral infection. Thus, in addition to their antioncogenic potential, EZH2/1 inhibitors may have a role as general antivirals for short-term enhancement of viral clearance, for suppression of persistent infections and drug-resistant mutants, or as enhancers of immune responses where no specific pharmaceutical antiviral is available. PRC2 epigenetic inhibitors currently in clinical trial for cancer therapy may provide an initial therapeutic resource for new or emerging pathogens (e.g., ZIKV). Viral yields. Viral DNA levels in genomic DNA from HFF cells and mouse tissues were determined by qPCR, and samples were normalized on the basis of the levels of the cellular GAPDH gene. The sequences of the primer sets used are listed in Text S1. Viral titers were determined from homogenates of mouse tissues or HFF cells.

MATERIALS AND METHODS
Reverse transcription-qPCR and qPCR. cDNA was synthesized from total RNA (Isolate II RNA minikit; Bioline) with a qScript cDNA synthesis kit (Quanta Biosciences) or a Maxima First Strand cDNA synthesis kit (Thermo Scientific). For ganglia, tissues were first homogenized in TriPure Reagent (Roche catalog no. 11667165001) with Lysing Matrix D (MP Biomedicals catalog no. 6913-100) and a FastPrep-24 Instrument (MP Biomedicals). DNAs or cDNAs were quantitated in triplicate by qPCR with SYBR green master mix (Roche) and a Mastercycler ep realplex4 (Eppendorf; Realplex 2.2v software) or a QuantStudio 3 (Applied Biosystems; QuantStudio 1.4v software). The sequences of the primer sets used are listed in Text S1. qPCR arrays, microarrays, and RNA-Seq. Total RNAs from mouse ganglia or HFF cells were isolated with an Isolate II RNA minikit (Bioline) as noted above. RNA quality (an RNA integrity number of Ͼ8.5) was verified by Bioanalyzer for all qPCR array, microarray, and RNA-Seq samples. Differentials in the EZH2/1 Inhibitors Suppress Diverse Viral Pathogens ® expression of genes for IFNs and IFN receptors were determined with RT 2 Profiler PCR Arrays (Qiagen catalog no. PAHS-064ZA) (three independent experiments). Microarrays were completed with Illumina Human HT-12 v4.0 Expression BeadChip as detailed in Text S1. RNA-Seq analyses (HiSeq2000/2500 system; Illumina) of libraries (TruSeq Stranded Total RNA with Ribo-Zero; Illumina) from three independent replicates were done as described in Text S1. Statistical analyses of microarray and RNA-Seq data are described in Text S1.
Western blot assays. Western blot assays of resolved nuclear protein extracts were done with the antibodies listed in Text S1. Blots were visualized with WesternBright Quantum (Advansta) and quantitated with a G:BOX Chemi XT4 (Syngene; GeneTools 4.03.02.0v software).
ChIP assays. ChIP assays were done as described in detail in Text S1. Animals and infections. (i) Trigeminal ganglion explants. BALB/c mice were infected with 5 ϫ 10 5 PFU of HSV-1 (strain F) via the ocular route as previously described (64). Latently infected trigeminal ganglia were harvested 30 to 45 days after clearance of the primary infection. Trigeminal ganglia were bisected, and paired halves were explanted in medium with the vehicle and inhibitors for 48 h.
(ii) Primary ocular and intranasal HSV infections. BALB/c mice were infected with 2 ϫ 10 5 PFU of HSV-1 (strain F)/eye. The vehicle, ACV (30 to 100 M), and EZH2/1 inhibitors (GSK126, 30 to 60 M; UNC1999, 15 M) were applied topically to the surface of the eye twice daily beginning at 12 h postinfection (hpi). Eyes were harvested on the days postinfection indicated to obtain viral yields and tissue sections. Mice were infected intranasally with 5 ϫ 10 5 PFU of HSV-1 (strain F) and treated i.p. with the vehicle, ACV (50 mg/kg) or GSK126 (50 mg/kg) beginning 1 day prior to infection. Viral yields from nasal tissue were determined at 7 dpi. Animal care and handling were done in accordance with the National Institutes of Health Animal Care and Use Guidelines and as approved by the NIAID Animal Care and Use Committee.
Immunofluorescence microscopy. Immunofluorescence staining with the antibodies listed in Text S1 was done in accordance with standard protocols. Cells were visualized with a Leica TCS SP5 confocal microscope with LASAF software (version 2.6.0). Deconvolution was completed with Huygens Essential (Scientific Volume Imaging, 15.10.1P6v), and sequential z sections were assembled with Imaris software (Bitplane AG, 8.1.2v).
Ganglion and ocular immunohistochemical staining. Latently HSV-1-infected trigeminal ganglia were explanted for 48 h into DMEM--10% FBS in the presence or absence of inhibitors. Explanted ganglia or primary infected eyes were fixed in 4% paraformaldehyde (PFA)--phosphate-buffered saline (PBS) and embedded in paraffin. Sections were stained with the antibodies listed in Text S1 as previously described (64). Sequential z-stack images were collected with a Leica TCS SP5 confocal microscope.
ZIKV intracellular staining assay. Vehicle-or EZH2/1 inhibitor-treated HFF cells were infected with ZIKV (30,000 FFU/2.2 ϫ 10 5 cells) for 40 h. Cells were fixed in Fix/Perm Solution (BD Biosciences) and stained with anti-ZIKV antibody ZV67. The percentage of infected cells was determined by flow cytometry.
Statistical analyses. Data are presented as means Ϯ the standard error of the mean (SEM). Where appropriate, statistical analyses (Prism 7.0) included analysis of variance (ANOVA) with Dunnett's post hoc test for multiple comparisons, two-tailed t tests for individual comparisons, and Wilcoxon matched-pair signed-rank tests for comparisons of paired vehicle-and compound-treated ganglia. Details are presented in Table S2.
Data availability. Microarray and RNA-Seq data sets are available at https://www.ncbi.nlm.nih.gov/ geo/ under accession number GSE99841.

ACKNOWLEDGMENTS
We thank J. Vogel, R. Alfonso, and M. O'Donoghue Altman (Laboratory of Viral Diseases, NIAID) for discussions and review of the manuscript and NIAID Bld33 Vivarium staff and A. Reed (Laboratory of Viral Diseases, NIAID) for excellent technical support.